scholarly journals Rabbit Achilles tendon full transection model – wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery

Biology Open ◽  
2016 ◽  
Vol 5 (9) ◽  
pp. 1324-1333 ◽  
Author(s):  
Gabriella Meier Bürgisser ◽  
Maurizio Calcagni ◽  
Elias Bachmann ◽  
Gion Fessel ◽  
Jess G. Snedeker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Achilles Tendon ◽  
Adhesion Formation ◽  
Post Surgery

scholarly journals Electrospun tube reduces adhesion in rabbit Achilles tendon 12 weeks post-surgery without PAR-2 overexpression

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Gabriella Meier Bürgisser ◽  
Olivera Evrova ◽  
Dorothea M. Heuberger ◽  
Petra Wolint ◽  
Julia Rieber ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Achilles Tendon ◽  
Adhesion Formation ◽  
Tendon Repair ◽  
Immunofluorescent Staining ◽  
Surrounding Tissue ◽  
Post Surgery ◽  
Post Operation

AbstractOne great challenge in surgical tendon repair is the minimization of peritendinous adhesions. An electrospun tube can serve as a physical barrier around a conventionally sutured tendon. Six New Zealand White rabbits had one Achilles tendon fully transsected and sutured by a 4-strand suture. Another six rabbits had the same treatment, but with the additional electrospun DegraPol tube set around the sutured tendon. The adhesion formation to the surrounding tissue was investigated 12 weeks post-operation. Moreover, inflammation-related protease-activated receptor-2 (PAR-2) protein expression was assessed. Finally, rabbit Achilles tenocyte cultures were exposed to platelet-derived growth factor-BB (PDGF-BB), which mimicks the tendon healing environment, where PAR-2 gene expression was assessed as well as immunofluorescent staining intensity for F-actin and α-tubulin, respectively. At 12 weeks post-operation, the partially degraded DegraPol tube exhibited significantly lower adhesion formation (− 20%). PAR-2 protein expression was similar for time points 3 and 6 weeks, but increased at 12 weeks post-operation. In vitro cell culture experiments showed a significantly higher PAR-2 gene expression on day 3 after exposure to PDGF-BB, but not on day 7. The cytoskeleton of the tenocytes changed upon PDGF-BB stimulation, with signs of reorganization, and significantly decreased F-actin intensity. An electrospun DegraPol tube significantly reduces adhesion up to twelve weeks post-operation. At this time point, the tube is partially degraded, and a slight PAR-2 increase was detected in the DP treated tendons, which might however arise from particles of degrading DegraPol that were stained dark brown. PAR-2 gene expression in rabbit tenocytes reveals sensitivity at around day 10 after injury.

scholarly journals A Novel Kartogenin-Releasing Polymer Scaffold Promotes Wounded Rat Achilles Tendon Enthesis Healing

2018 ◽  
Vol 3 (3) ◽  
pp. 2473011418S0053
Author(s):  
Jianying Zhang ◽  
Daibang Nie ◽  
Guangyi Zhao ◽  
Susheng Tan ◽  
MaCalus Hogan ◽  
...  
Keyword(s):  
Achilles Tendon ◽  
Saline Group ◽  
Future Research ◽  
Dependent Manner ◽  
Polymer Scaffold ◽  
Normal Medium ◽  
Post Surgery ◽  
Tendon Stem Cells

Category: Hindfoot Introduction/Purpose: Entheses have a special fibrocartilage transition zone where tendons and ligaments attach to bone. Enthesis injury is very common, and the reattachment of tendon to bone is a great challenge because healing takes place between a soft tissue (tendon) and a hard tissue (bone). We have now developed a kartogene (KGN)-containing polymer scaffold (KGN-P) that can precisely deliver KGN to damaged enthesis area. The effects of the KGN-containing polymer on the healing of wounded TBJ were investigated in vitro and in vivo. Methods: The proliferation and chondrogenesis of rat Achilles tendon stem cells (TSCs) grown in four conditions were measured: normal medium (Control); normal medium with 100 nM KGN (KGN); lysine diisocyanate (LDI)-glycerol scaffold with normal medium (LDI-P); LDI-glycerol-KGN scaffold with normal medium (KGN-P).A wound (1 mm) was created on each hind leg Achilles enthesis of all 8 rats (3 months old). The wounds were then treated either with 10 ul saline (Wound); or 10 ul of 10 uM KGN (KGN); or LDI polymer scaffold (LDI-P); or KGN-containing polymer scaffold (KGN-P). The rats were sacrificed on day 15 and 30 post-surgery, and their Achilles entheses were collected for gross inspection and histochemical analysis. Results: KGN-containing polymers have sponge-like structures (Fig. 1A-D), and release KGN in a time- and temperature-dependent manner (Fig. 1E). KGN-P scaffold induced chondrogenesis of TSCs (Fig. 2D, 2H) without changing cell proliferation (Fig. 2I), and enhanced fibrocartilage-like tissue formation (Fig. 3E). KGN (Fig. 3C) and LDI-P (Fig. 3D) treated groups exhibited unhealed wound areas as in saline group (Fig. 3B). Finally, KGN-P and KGN treated rat TSCs underwent chondrogenesis by upregulating collagen II, aggrecan, and SOX-9 expression (Fig. 3F). Conclusion: Our results showed that KGN-containing polymer scaffold enhanced wounded enthesis healing by inducing TSC chondrogenesis and promoting the formation of the fibrocartilage in the wound site. The KGN-P may be used for regeneration of wounded entheses in clinical settings. Future research will focus on optimizing KGN concentration and releasing rate in the polymer scaffold during enthesis healing.

scholarly journals A comparative study between fistulotomy and fistulectomy in management of low anal fistulae

2017 ◽  
Vol 4 (11) ◽  
pp. 3665 ◽  
Author(s):  
Ganesan R. ◽  
Karunakaran K. ◽  
Heber Anandan
Keyword(s):  
Wound Healing ◽  
Operating Time ◽  
Healing Time ◽  
Human Beings ◽  
Fistula In Ano ◽  
Post Surgery ◽  
Group I ◽  
Hospital Stays ◽  
Group Ii ◽  
Wound Healing Time

Background: Fistula in ano is a silent menace in human beings, the treatment is a challenging one even for experienced surgeons, fistula in ano forms a good majority of treatable benign lesions of rectum and anal canal. Aim was to study the efficacy of fistulotomy and fistulectomy in the treatment of low anal fistulae.Methods: A randomized control study was conducted to compare fistulotomy with fistulectomy in patients with low anal fistulae.Results: The operating time in fistulotomy group was 12.13minutes±2.11minutes and in fistulectomy group was 22.23±3.36minutes. The post-surgery hospital stays in Group I was 1.80±0.66days and in Group II was 2.60±0.563 days. The wound healing time in Group I was 24.20±2.95 days which was considerably less when compared to patients in Group II where it was 31.50±4.34 days.Conclusions: Fistulotomy has a slight edge over fistulectomy in the treatment of low anal fistulas since it has shorter operating time, less post-operative pain, less complications, quicker wound healing time, less incontinence and a comparable recurrence rate.

scholarly journals Development of a Novel Pre-Vascularized Three-Dimensional Skin Substitute Using Blood Plasma Gel

2018 ◽  
Vol 27 (10) ◽  
pp. 1535-1547 ◽  
Author(s):  
Niann-Tzyy Dai ◽  
Wen-Shyan Huang ◽  
Fang-Wei Chang ◽  
Lin-Gwei Wei ◽  
Tai-Chun Huang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Plasma ◽  
Three Dimensional ◽  
Skin Substitute ◽  
Strength Analysis ◽  
Full Thickness ◽  
Skin Substitutes ◽  
Full Thickness Skin ◽  
Post Surgery ◽  
Thickness Skin

Skin substitutes with existing vascularization are in great demand for the repair of full-thickness skin defects. In the present study, we hypothesized that a pre-vascularized skin substitute can potentially promote wound healing. Novel three-dimensional (3D) skin substitutes were prepared by seeding a mixture of human endothelial progenitor cells (EPCs) and fibroblasts into a human plasma/calcium chloride formed gel scaffold, and seeding keratinocytes onto the surface of the plasma gel. The capacity of the EPCs to differentiate into a vascular-like tubular structure was evaluated using immunohistochemistry analysis and WST-8 assay. Experimental studies in mouse full-thickness skin wound models showed that the pre-vascularized gel scaffold significantly accelerated wound healing 7 days after surgery, and resembled normal skin structures after 14 days post-surgery. Histological analysis revealed that pre-vascularized gel scaffolds were well integrated in the host skin, resulting in the vascularization of both the epidermis and dermis in the wound area. Moreover, mechanical strength analysis demonstrated that the healed wound following the implantation of the pre-vascularized gel scaffolds exhibited good tensile strength. Taken together, this novel pre-vascularized human plasma gel scaffold has great potential in skin tissue engineering.

scholarly journals Effects of Lactobacillus reuteri-containing lozenges on healing after surgical removal of mandibular third molars: a randomised controlled trial

2019 ◽  
Vol 10 (6) ◽  
pp. 653-659
Author(s):  
D.-Å. Wälivaara ◽  
I. Sjögren ◽  
N. Gerasimcik ◽  
T. Yucel-Lindberg ◽  
S. Twetman ◽  
...  
Keyword(s):  
Wound Healing ◽  
Lactobacillus Reuteri ◽  
Controlled Trial ◽  
Test Group ◽  
Subjective Perception ◽  
Surgical Removal ◽  
Third Molars ◽  
Post Surgery ◽  
Surgical Extraction ◽  
Mandibular Third Molars

We investigated the effect of probiotic supplements on oral wound healing, swelling, pain and discomfort after surgical removal of mandibular third molars. A second aim was to evaluate if the intervention could influence the concentrations of oxytocin in saliva. Sixty-four consecutive volunteers (18-34 years) were enrolled to a double-blind randomised placebo-controlled trial with two parallel arms. Following surgery, the patients were asked to take three lozenges per day containing two strains of Lactobacillus reuteri (DSM 17938 and ATCC PTA 5289) or placebo for two weeks. The clinical healing and extra-oral swelling were scored two weeks post-operatively. Samples of wound exudate were cultivated for the presence of Staphylococcus aureus and β-haemolytic streptococci. Salivary oxytocin concentrations were analysed from pre- and post-surgery samples using ELISA technique. Compliance and the subjective perception of swelling, pain and discomfort were reported daily through visual analogue scales in a logbook. All patients except three completed the protocol and the postoperative course was uneventful in most cases. Minor extra-oral swellings were noted in five patients, but none required antibiotic treatment. At the 2-week follow-up, there were no significant differences in clinical wound healing index, extra-oral swelling, bacterial growth or salivary oxytocin levels between the groups. The self-reported data unveiled, however, a significantly reduced sense of swelling, in particular during the second week after surgery in the probiotic test group (P<0.05). Likewise, significantly fewer nights with disturbed sleep and fewer days with sick-leave from work were reported among the participants in the test group (P<0.05). No differences were found in the post-operative use of analgesics. In conclusion, we found no significant influence of probiotic supplements on objective wound healing after surgical extraction of impacted mandibular third molars. However, since the patients’ perceived significant post-operative ameliorations, further studies are needed to explore the patient’s value of the intervention.

Influence of intraperitoneal phospholipid dosage on adhesion formation and wound healing at different intervals after surgery

2001 ◽  
Vol 386 (4) ◽  
pp. 278-284 ◽  
Author(s):  
Stefan A. Müller ◽  
Karl H. Treutner ◽  
Lothar Tietze ◽  
Michail Anurov ◽  
Svetlana Titkova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adhesion Formation

scholarly journals Differences of Reeda Scale in Wound Incision Abdomen Post Obstetrics / Gynaecology Laparotomy with Topical Virgin Coconut Oil (VCO) and Without Topical Virgin Coconut Oil (VCO)

2021 ◽  
Vol 5 (1) ◽  
pp. 90-101
Author(s):  
Nanda Tri Wahdini ◽  
Ferdinal Ferry ◽  
Sumaryati Syukur
Keyword(s):  
Wound Healing ◽  
Treatment Group ◽  
Coconut Oil ◽  
Care Group ◽  
Virgin Coconut Oil ◽  
Abdominal Incision ◽  
Post Surgery ◽  
Surgical Wounds ◽  
The Mean

Introduction : Wounds are defects of normal anatomical structures and functions. Injuries can occur because of physical, chemical, or biological trauma. Wound healing is a complex process involving many cells, cytokines, growth factors, proteases, and extracellular matrix (ECM) that work together to restore the integrity of the injured tissue. Virgin Coconut Oil is an oil saturated with medium-chain fatty acids (MCFA), one of which is lauric acid. Based on our research, VCO has wound healing properties. Research conducted in vivo on rabbits and rats experienced an increase in collagen, fibroblast proliferation, and neovascularization which influenced accelerating wound healing.Objective : This study aims to determine the difference in the mean REEDA scale in abdominal incision wounds after obstetric / gynecology laparotomy with topical VCO and those without topical VCO.Material and methods : This study is an experimental research design with a randomized clinical trial that was conducted on 36 people divided into 2 groups (treatment and control). Post-obstetric / gynecology laparotomy patients were subjected to topical VCO application on the incision wound and the REEDA scale was calculated at 24, 48, and 72 hours post-surgery. Statistical analysis is using the Mann-Whitney test.Results : Age mean of the respondents in the treatment group was 32.28 ± 10.42 years old, while the untreated group was 30.5 ± 6.54 years old. The mean REEDA scale for abdominal incision wounds applied topical VCO at 24 hours was 3.17 ± 1.04, at 48 hours was 2.11 ± 1.08, and at 72 hours was 1.39 ± 0.85. The mean REEDA scale for abdominal incision wounds that were not treated with topical VCO at 24 hours was 3.83 ± 0.99, at 48 hours was 3.11 ± 1.37 and at 72 hours was 2.72 ± 1.41. The findings of the experimental analyses showed that there was no association with the use of topical VCO in surgical wounds on a 24-hour REEDA scale (p>0.05), there was a relationship between the use of topical VCO in surgical wounds on a 48-hour and 72-hour REEDA scale (p<0.05). In the care group, the mean REEDA scale decreased by 33.4 % from 24 to 48 hours and 34.1 % from 48 to 72 hours.Conclusion : There is a relationship with the use of topical VCO in post-abdominal incision wounds post obstetrics/gynaecology laparotomy with REEDA scale 48 hours and 72 hours post-surgery. The mean REEDA scale decreased by over 24 to 48 hours and 48 to 72 hours in the treatment group (who administered topical VCO in postoperative incision wounds).Keywords: VCO, REEDA scale, Wound healing

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