Influence of intraperitoneal phospholipid dosage on adhesion formation and wound healing at different intervals after surgery

2001 ◽  
Vol 386 (4) ◽  
pp. 278-284 ◽  
Author(s):  
Stefan A. Müller ◽  
Karl H. Treutner ◽  
Lothar Tietze ◽  
Michail Anurov ◽  
Svetlana Titkova ◽  
...  
Keyword(s):  
Wound Healing ◽  
Adhesion Formation

scholarly journals Rho kinases regulate corneal epithelial wound healing

2008 ◽  
Vol 295 (2) ◽  
pp. C378-C387 ◽  
Author(s):  
Jia Yin ◽  
Fu-Shin X. Yu
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Cell Migration ◽  
Rho Gtpase ◽  
Adhesion Formation ◽  
Small Gtpase ◽  
Corneal Epithelial Cell ◽  
Corneal Epithelial ◽  
Epithelial Wound Healing ◽  
Rho Kinases

We have previously shown that Rho small GTPase is required for modulating both cell migration and proliferation through cytoskeleton reorganization and focal adhesion formation in response to wounding. In the present study, we investigated the role of Rho kinases (ROCKs), major effectors of Rho GTPase, in mediating corneal epithelial wound healing. Both ROCK 1 and 2 were expressed and activated in THCE cells, an SV40-immortalized human corneal epithelial cell (HCEC) line, in response to wounding, lysophosphatidic acid, and heparin-binding EGF-like growth factor (HB-EGF) stimulations. The ROCK inhibitor Y-27632 efficiently antagonized ROCK activities without affecting Rho activation in wounded HCECs. Y-27632 promoted basal and HB-EGF-enhanced scratch wound healing and enhanced cell migration and adhesion to matrices, while retarded HB-EGF induced cell proliferation. E-cadherin- and β-catenin-mediated cell-cell junction and actin cytoskeleton organization were disrupted by Y-27632. Y-27632 impaired the formation and maintenance of tight junction barriers indicated by decreased trans-epithelial resistance and disrupted occludin staining. We conclude that ROCK activities enhance cell proliferation, promote epithelial differentiation, but negatively modulate cell migration and cell adhesion and therefore play a role in regulating corneal epithelial wound healing.

scholarly journals Rabbit Achilles tendon full transection model – wound healing, adhesion formation and biomechanics at 3, 6 and 12 weeks post-surgery

Biology Open ◽  
2016 ◽  
Vol 5 (9) ◽  
pp. 1324-1333 ◽  
Author(s):  
Gabriella Meier Bürgisser ◽  
Maurizio Calcagni ◽  
Elias Bachmann ◽  
Gion Fessel ◽  
Jess G. Snedeker ◽  
...  
Keyword(s):  
Wound Healing ◽  
Achilles Tendon ◽  
Adhesion Formation ◽  
Post Surgery

scholarly journals Reliability of combination of hyaluronic acid application and early rehabilitation in prevention of postoperative adhesions after primary tendon repair

2020 ◽  
Vol 7 (2) ◽  
pp. 343
Author(s):  
Medhat S. Hassan ◽  
Alaa A. Labeeb
Keyword(s):  
Wound Healing ◽  
Hyaluronic Acid ◽  
Reliable Method ◽  
Foreign Body Reaction ◽  
Adhesion Formation ◽  
Tendon Repair ◽  
Postoperative Adhesions ◽  
Tissue Adhesion ◽  
Burn Surgery

Background: The aim of the study was to evaluate the efficacy of hyaluronic acid application after tendon repair in prevention of postoperative adhesions, and to clarify the best circumstances that must be fulfilled to obtain this goal. Tissue adhesion usually happens due to injury, foreign-body reaction, bleeding, or infection but is considered an important factor for wound healing. As a result, tendons repair after injury is usually complicated due to limited postoperative movement. Several methods were used to try to prevent postoperative adhesions but most of them did not give satisfactory results as the mechanism of adhesion formation is still unclear.Methods: Fifty patients were included in this prospective study performed at department of plastic, reconstruction and burn surgery, faculty of medicine, Menoufia University over the period of approximately two years (January 2016 to November 2017).Results: Close follow up of the function of the repaired tendons was evaluated repeatedly over the period of 6 months. 40 patients showed excellent progress in regaining function with less possibility of performing adhesions, these patients also expressed satisfaction and comfort toward the regained function. 10 patients showed reasonable progress and were mildly satisfied.Conclusions: Application of hyaluronic acid in addition to a well-planned rehabilitation plan is a good and reliable method against adhesions when performing primary tendon repair.

scholarly journals Comparison of Absorbable Packing versus No Packing in Wound Healing after Endoscopic Sinus Surgery: A Systematic Review and Pooled Analysis

ORL ◽  
2021 ◽  
pp. 1-8
Author(s):  
Zhenxiao Huang ◽  
Bing Zhou
Keyword(s):  
Systematic Review ◽  
Wound Healing ◽  
Endoscopic Sinus Surgery ◽  
Adhesion Formation ◽  
Pooled Analysis ◽  
Nasal Packing ◽  
Sinus Surgery ◽  
Cochrane Library ◽  
Significant Difference ◽  
Mucosal Edema

<b><i>Introduction:</i></b> Nasal packing after endoscopic sinus surgery (ESS) is controversial. The aim of this systematic review was to compare absorbable packing versus no packing in wound healing after ESS for the treatment of chronic rhinosinusitis. <b><i>Data Source:</i></b> English electronic databases, including Cochrane Library, EMBASE, MEDLINE, and PubMed, were searched, and only randomized controlled trials were included. <b><i>Methods:</i></b> The outcome measures were the presence of synechiae/adhesion formation, mucosal edema, crusting, granulation formation, and infection. The McNemar’s test was used for pooled analysis. <b><i>Results:</i></b> Four studies with 148 participants were included. The pooled analysis showed that absorbable packing may offer benefit in reducing adhesion at 6–8 weeks (odds ratio [OR]: 0.3864; 95% confidence interval [CI]: 0.2136–0.7235) and 12 weeks (OR: 0.2396, 95% CI: 0.08267–0.7709) postoperatively compared with no packing. There was no significant difference between the packed and the unpacked side at 2, 6–8, and 12 weeks after ESS in terms of presence of crusting, edema, and granulation formation. <b><i>Conclusion:</i></b> There is insufficient evidence to suggest that absorbable packing after ESS does not increase mucosal edema, granulation formation, and infection. However, the absorbable packing may be more effective than no packing for the prevention of mucosal adhesion after ESS. The use of absorbable nasal packing after ESS is recommended when used to reduce postoperative mucosal adhesion. More research in this area is clearly needed.

scholarly journals Inhibition of fibrous adhesion formation in the temporomandibular joint of tenascin-C knockout mice

2014 ◽  
Author(s):  
Y. Shinohara ◽  
K. Okamoto ◽  
Y. Goh ◽  
N. Kiga ◽  
I. Tojyo ◽  
...  
Keyword(s):  
Wound Healing ◽  
Temporomandibular Joint ◽  
Smooth Muscle Actin ◽  
Adhesion Formation ◽  
Mouth Opening ◽  
Wild Type ◽  
Tenascin C ◽  
Protein Levels ◽  
Opening Method

Tenascin-C (TNC) is a large hexameric extracellular matrix glycoprotein that is expressed in developing organs and tumors. It has been reported that TNC is expressed in inflamed synovial membranes and deformed discs of temporomandibular joint (TMJ) disorder. However, the role of TNC in TMJ is not fully known. In this study, the role of TNC in fibrous adhesion formation of TMJ was examined using TNC knockout (TNCKO) mice. Hypermobility was produced by excessive mouth opening method on the TMJ of both wild-type (WT) and TNCKO mice. TMJ wound healing was compared histologically, and the expression of TNC, fibronectin (FN) and α-smooth muscle actin (α-SMA) in the wounded TMJ was examined by immunohistochemical and immunoblot analyses. Based on histologic analysis, fibrous adhesions were observed in the TMJ of both TNCKO and wild-type (WT) mice after excessive mouth opening. However, fibrous adhesion formation in TNCKO mice occurred later than in WT mice. TNC was expressed in the wounded TMJ disc and mandibular fossa. Although FN and α-SMA expression in the TMJ of TNCKO and WT mice was up-regulated after excessive mouth opening, FN and α-SMA protein levels were higher in WT mice at the same time points. In the wounded TMJ, TNC appears to enhance the expression of FN and α-SMA, and a lack of TNC may reduce fibrous adhesion formation in the TMJ. TNC plays an important role in TMJ wound healing, especially for wounds generated by mechanical stress.

scholarly journals Sterile Injury Repair and Adhesion Formation at Serosal Surfaces

2021 ◽  
Vol 12 ◽  
Author(s):  
Simone N. Zwicky ◽  
Deborah Stroka ◽  
Joel Zindel
Keyword(s):  
Wound Healing ◽  
Immune Cells ◽  
Wound Repair ◽  
Critical Role ◽  
Adhesion Formation ◽  
Clinical Importance ◽  
Body Cavity ◽  
Sterile Inflammation ◽  
Damage Recognition

Most multicellular organisms have a major body cavity containing vital organs. This cavity is lined by a mucosa-like serosal surface and filled with serous fluid which suspends many immune cells. Injuries affecting the major body cavity are potentially life-threatening. Here we summarize evidence that unique damage detection and repair mechanisms have evolved to ensure immediate and swift repair of injuries at serosal surfaces. Furthermore, thousands of patients undergo surgery within the abdominal and thoracic cavities each day. While these surgeries are potentially lifesaving, some patients will suffer complications due to inappropriate scar formation when wound healing at serosal surfaces defects. These scars called adhesions cause profound challenges for health care systems and patients. Therefore, reviewing the mechanisms of wound repair at serosal surfaces is of clinical importance. Serosal surfaces will be introduced with a short embryological and microanatomical perspective followed by a discussion of the mechanisms of damage recognition and initiation of sterile inflammation at serosal surfaces. Distinct immune cells populations are free floating within the coelomic (peritoneal) cavity and contribute towards damage recognition and initiation of wound repair. We will highlight the emerging role of resident cavity GATA6+ macrophages in repairing serosal injuries and compare serosal (mesothelial) injuries with injuries to the blood vessel walls. This allows to draw some parallels such as the critical role of the mesothelium in regulating fibrin deposition and how peritoneal macrophages can aggregate in a platelet-like fashion in response to sterile injury. Then, we discuss how serosal wound healing can go wrong, causing adhesions. The current pathogenetic understanding of and potential future therapeutic avenues against adhesions are discussed.

Sign in / Sign up

Export Citation Format

Share Document