scholarly journals Paracrine Pathways in Uterine Leiomyoma Stem Cells Involve Insulinlike Growth Factor 2 and Insulin Receptor A

2017 ◽  
Vol 102 (5) ◽  
pp. 1588-1595 ◽  
Author(s):  
Molly B. Moravek ◽  
Ping Yin ◽  
John S. Coon ◽  
Masanori Ono ◽  
Stacy A. Druschitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Insulin Receptor ◽  
Uterine Leiomyoma ◽  
Insulinlike Growth Factor

scholarly journals Low Oxygen Tension Modulates the Insulin-Like Growth Factor-1 or -2 Signaling via Both Insulin-Like Growth Factor-1 Receptor and Insulin Receptor to Maintain Stem Cell Identity in Placental Mesenchymal Stem Cells

Endocrinology ◽  
2016 ◽  
Vol 157 (3) ◽  
pp. 1163-1174 ◽  
Author(s):  
Amer Youssef ◽  
Victor K. M. Han
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Growth Factor ◽  
Insulin Receptor ◽  
Oxygen Tension ◽  
Insulin Like Growth Factor ◽  
Low Oxygen ◽  
Placental Mesenchymal Stem Cells ◽  
Low Oxygen Tension

Abstract Placental mesenchymal stem cells (PMSCs) are readily available multipotent stem cells for potential use in regenerative therapies. For this purpose, PMSCs must be maintained in culture conditions that mimic the in vivo microenvironment. IGFs (IGF-1 and IGF-2) and oxygen tension are low in the placenta in early gestation and increase as pregnancy progresses. IGFs bind to two receptor tyrosine kinases, the IGF-1 receptor (IGF-1R) and the insulin receptor (IR), and their hybrid receptors. We hypothesized that IGF-1 and IGF-2 signal via distinct signaling pathways under low-oxygen tension to maintain PMSC multipotency. In preterm PMSCs, low-oxygen tension increased the expression of IGF-2 and reduced IGF-1. IGF-1 stimulated higher phosphorylation of IGF-1Rβ, ERK1/2, and AKT, which was maintained at steady lower levels by low oxygen tension. PMSC proliferation was increased by IGF-1 more than IGF-2,and was potentiated by low-oxygen tension. This IGF/low oxygen tension-mediated proliferation was receptor dependent because neutralization of the IGF-1R inhibited PMSC proliferation in the presence of IGF-1 and the IR in presence of IGF-2. These findings suggest that both IGF-1R and the IR can participate in mediating IGF signaling in maintaining PMSCs multipotency. We conclude that low-oxygen tension can modify the IGF-1 or IGF-2 signaling via the IGF-1R and IR in PMSCs.

Inability of insulin and insulinlike growth factor-1 to stimulate sugar or amino acid transport and thymidine incorporation in cultured myeloma cells

1991 ◽  
Vol 69 (12) ◽  
pp. 859-863 ◽  
Author(s):  
Blaine Leckett ◽  
Ralph J. Germinario
Keyword(s):  
Amino Acid ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Insulin Receptor ◽  
Amino Acid Transport ◽  
Sugar Transport ◽  
Acid Transport ◽  
Myeloma Cells ◽  
Insulinlike Growth Factor ◽  
Stimulation Of

NS-1 mouse plasmacytoma cells were examined for their insulin and insulinlike growth factor-1 (IGF-1) binding characteristics and ability to produce peptide-dependent cellular effects. At concentrations of labelled insulin (i.e., 1.7 × 10−10 M) or IGF-1 (i.e., 1.5 × 10−10 M), NS-1 cells specifically bind 0.2 ± 0.06 fmol insulin per 106 cells (n = 7), where little, if any, IGF-1 specific binding was observed (0.02 ± 0.01 fmol/106 cells) (n = 3). Additionally, the data indicate that the total number of insulin binding sites per cell was 3200 ± 390 (n = 3). Insulin was employed at various concentrations (6.7–667 nM) and failed to stimulate either sugar or amino acid transport. Insulin at low concentrations (i.e., 6.7 or 67 nM) did not stimulate DNA synthesis, yet a small but significant increase was observed at a concentration of 667 nM insulin. IGF-1 did not stimulate DNA synthesis at all concentrations employed (1.4–143 nM). In summary, there exists a small but significant number of insulin receptors, little insulin-stimulated DNA synthesis, and no apparent insulin stimulation of sugar or amino acid transport. Also, since there is no significant IGF-1 binding and no IGF-1 stimulation of DNA synthesis, these findings indicate that this cell line might be a good candidate for the study of insulin receptor function as a transfection recipient of insulin receptor genes.Key words: cultured myeloma cells, insulin and IGF-1 binding and action, sugar transport, amino acid transport, DNA synthesis.

Insulinlike growth factor action and production in adipocytes and endothelial cells from human adipose tissue

Diabetes ◽  
1989 ◽  
Vol 38 (6) ◽  
pp. 710-717 ◽  
Author(s):  
P. A. Kern ◽  
M. E. Svoboda ◽  
R. H. Eckel ◽  
J. J. Van Wyk
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Insulinlike Growth Factor

Lack of growth hormone effect on insulin-associated suppression of insulinlike growth factor binding protein 1 in humans

Diabetes ◽  
1990 ◽  
Vol 39 (10) ◽  
pp. 1251-1256 ◽  
Author(s):  
C. A. Conover ◽  
P. C. Butler ◽  
M. Wang ◽  
R. A. Rizza ◽  
P. D. Lee
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Binding Protein ◽  
Factor Binding ◽  
Insulinlike Growth Factor ◽  
Hormone Effect

Experimental diabetes increases insulinlike growth factor I and II receptor concentration and gene expression in kidney

Diabetes ◽  
1990 ◽  
Vol 39 (12) ◽  
pp. 1490-1497 ◽  
Author(s):  
H. Werner ◽  
Z. Shen-Orr ◽  
B. Stannard ◽  
B. Burguera ◽  
C. T. Roberts ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Experimental Diabetes ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Receptor Concentration ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I
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