scholarly journals Direct Demonstration of Separate Receptors for Growth and Metabolic Activities of Insulin and Multiplication-stimulating Activity (an Insulinlike Growth Factor) Using Antibodies to the Insulin Receptor

1980 ◽  
Vol 66 (1) ◽  
pp. 130-140 ◽  
Author(s):  
George L. King ◽  
C. Ronald Kahn ◽  
Matthew M. Rechler ◽  
S. Peter Nissley
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Insulinlike Growth Factor ◽  
Direct Demonstration ◽  
Metabolic Activities

scholarly journals Paracrine Pathways in Uterine Leiomyoma Stem Cells Involve Insulinlike Growth Factor 2 and Insulin Receptor A

2017 ◽  
Vol 102 (5) ◽  
pp. 1588-1595 ◽  
Author(s):  
Molly B. Moravek ◽  
Ping Yin ◽  
John S. Coon ◽  
Masanori Ono ◽  
Stacy A. Druschitz ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factor ◽  
Insulin Receptor ◽  
Uterine Leiomyoma ◽  
Insulinlike Growth Factor

Inability of insulin and insulinlike growth factor-1 to stimulate sugar or amino acid transport and thymidine incorporation in cultured myeloma cells

1991 ◽  
Vol 69 (12) ◽  
pp. 859-863 ◽  
Author(s):  
Blaine Leckett ◽  
Ralph J. Germinario
Keyword(s):  
Amino Acid ◽  
Growth Factor ◽  
Dna Synthesis ◽  
Insulin Receptor ◽  
Amino Acid Transport ◽  
Sugar Transport ◽  
Acid Transport ◽  
Myeloma Cells ◽  
Insulinlike Growth Factor ◽  
Stimulation Of

NS-1 mouse plasmacytoma cells were examined for their insulin and insulinlike growth factor-1 (IGF-1) binding characteristics and ability to produce peptide-dependent cellular effects. At concentrations of labelled insulin (i.e., 1.7 × 10−10 M) or IGF-1 (i.e., 1.5 × 10−10 M), NS-1 cells specifically bind 0.2 ± 0.06 fmol insulin per 106 cells (n = 7), where little, if any, IGF-1 specific binding was observed (0.02 ± 0.01 fmol/106 cells) (n = 3). Additionally, the data indicate that the total number of insulin binding sites per cell was 3200 ± 390 (n = 3). Insulin was employed at various concentrations (6.7–667 nM) and failed to stimulate either sugar or amino acid transport. Insulin at low concentrations (i.e., 6.7 or 67 nM) did not stimulate DNA synthesis, yet a small but significant increase was observed at a concentration of 667 nM insulin. IGF-1 did not stimulate DNA synthesis at all concentrations employed (1.4–143 nM). In summary, there exists a small but significant number of insulin receptors, little insulin-stimulated DNA synthesis, and no apparent insulin stimulation of sugar or amino acid transport. Also, since there is no significant IGF-1 binding and no IGF-1 stimulation of DNA synthesis, these findings indicate that this cell line might be a good candidate for the study of insulin receptor function as a transfection recipient of insulin receptor genes.Key words: cultured myeloma cells, insulin and IGF-1 binding and action, sugar transport, amino acid transport, DNA synthesis.

Insulinlike growth factor action and production in adipocytes and endothelial cells from human adipose tissue

Diabetes ◽  
1989 ◽  
Vol 38 (6) ◽  
pp. 710-717 ◽  
Author(s):  
P. A. Kern ◽  
M. E. Svoboda ◽  
R. H. Eckel ◽  
J. J. Van Wyk
Keyword(s):  
Endothelial Cells ◽  
Adipose Tissue ◽  
Growth Factor ◽  
Human Adipose Tissue ◽  
Insulinlike Growth Factor

Lack of growth hormone effect on insulin-associated suppression of insulinlike growth factor binding protein 1 in humans

Diabetes ◽  
1990 ◽  
Vol 39 (10) ◽  
pp. 1251-1256 ◽  
Author(s):  
C. A. Conover ◽  
P. C. Butler ◽  
M. Wang ◽  
R. A. Rizza ◽  
P. D. Lee
Keyword(s):  
Growth Hormone ◽  
Growth Factor ◽  
Binding Protein ◽  
Factor Binding ◽  
Insulinlike Growth Factor ◽  
Hormone Effect

Experimental diabetes increases insulinlike growth factor I and II receptor concentration and gene expression in kidney

Diabetes ◽  
1990 ◽  
Vol 39 (12) ◽  
pp. 1490-1497 ◽  
Author(s):  
H. Werner ◽  
Z. Shen-Orr ◽  
B. Stannard ◽  
B. Burguera ◽  
C. T. Roberts ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Factor ◽  
Experimental Diabetes ◽  
Factor I ◽  
Insulinlike Growth Factor ◽  
Receptor Concentration ◽  
Growth Factor I ◽  
Insulinlike Growth Factor I

scholarly journals Comparison of insulin-like growth factor I receptor and insulin receptor purified from human placental membranes.

1986 ◽  
Vol 261 (35) ◽  
pp. 16727-16731
Author(s):  
Y Fujita-Yamaguchi ◽  
T R LeBon ◽  
M Tsubokawa ◽  
W Henzel ◽  
S Kathuria ◽  
...  
Keyword(s):  
Growth Factor ◽  
Insulin Receptor ◽  
Insulin Like Growth Factor ◽  
Factor I ◽  
Placental Membranes ◽  
Growth Factor I
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