scholarly journals Metabolic Signature Shift in Type 2 Diabetes Mellitus Revealed by Mass Spectrometry-based Metabolomics

2013 ◽  
Vol 98 (6) ◽  
pp. E1060-E1065 ◽  
Author(s):  
Fengguo Xu ◽  
Subramaniam Tavintharan ◽  
Chee Fang Sum ◽  
Kaing Woon ◽  
Su Chi Lim ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Metabolic Signature

scholarly journals A Novel Diagnostic Biomarker, PZP, for Detecting Colorectal Cancer in Type 2 Diabetes Mellitus Patients Identified by Serum-Based Mass Spectrometry

2021 ◽  
Vol 8 ◽  
Author(s):  
Jiayue Yang ◽  
Weigang Fang ◽  
Wenjun Wu ◽  
Zhen Tian ◽  
Rong Gao ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Colorectal Cancer ◽  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Diagnostic Value ◽  
Serum Samples ◽  
Data Independent Acquisition ◽  
Ms Analysis

Background: Growing evidence has confirmed that populations with type 2 diabetes mellitus (T2DM) have an increasing risk of developing colorectal cancer (CRC). Thus, convenient and effective screening strategies for CRC should be developed for the T2DM population to increase the detection rate of CRC.Methods: Twenty serum samples extracted from five healthy participants, five T2DM patients, five CRC patients and five T2DM patients with CRC (T2DM + CRC) were submitted to data-independent acquisition mass spectrometry (DIA-MS) analysis to discover unique differentially altered proteins (DAPs) for CRC in patients with T2DM. Then, the diagnostic value of pregnancy zone protein (PZP) was validated by ELISA analysis in the validated cohort.Results: Based on DIA-MS analysis, we found eight unique proteins specific to T2DM patients with CRC. Among these proteins, four proteins showed different expression between the T2DM + CRC and T2DM groups, and PZP exhibited the largest difference. Next, the diagnostic value of serum PZP was validated by ELISA analysis with an AUC of 0.713. Moreover, the combination of PZP, CA199 and CEA exhibited encouraging diagnostic value, and the AUC reached 0.916.Conclusion: Overall, our current research implied that PZP could be regarded as a newfound serum biomarker for CRC medical diagnosis in T2DM patients.

scholarly journals Assessment of the amino acid profile in Thai patients with type 2 diabetes mellitus using liquid chromatography-mass spectrometry

2020 ◽  
Author(s):  
Natthida Sriboonvorakul ◽  
Wirichada Pan-Ngum ◽  
Kittiyod Poovorawan ◽  
Markus Winterberg ◽  
Joel Tarning ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Amino Acid ◽  
Glutamic Acid ◽  
Amino Acid Profile ◽  
Liquid Chromatography Mass Spectrometry ◽  
Subgroup Analyses

Abstract Background Type 2 diabetes mellitus (T2DM) is a global health problem. Early identification of those at risk is necessary to prevent its onset through lifestyle and pharmacologic interventions. T2DM is characterized by metabolic abnormalities, including protein metabolism. Evaluation of the amino acid profile might be beneficial for early assessment. Methods Liquid chromatography-mass spectrometry was performed to separate and quantify plasma amino acids from two groups of Thai individuals, patients with T2DM (n=103) and healthy individuals (n=104). Multivariate analysis was applied to compare free amino acid levels between groups. Subgroup analyses of patients with T2DM were performed to assess the association between amino acid profiles and important T2DM clinical characteristics. Results The multivariate analysis showed that glutamic acid was significantly associated with T2DM (OR 1.113, 95% CI 1.006 to 1.231) and results from the subgroup analyses showed that this correlation was significant in all subgroups of patients (p<0.05). Conclusions This finding needs to be confirmed in larger groups of patients with T2DM to explore glutamic acid as a biomarker for early prevention in particular at-risk groups. An in-depth understanding of the involvement of glutamic acid in T2DM could enhance our understanding of the disease and potentially provide novel interventions.

Gluconeogenesis in moderately and severely hyperglycemic patients with type 2 diabetes mellitus

2001 ◽  
Vol 280 (1) ◽  
pp. E23-E30 ◽  
Author(s):  
Guenther Boden ◽  
Xinhua Chen ◽  
T. Peter Stein
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Endogenous Glucose Production ◽  
Gas Chromatography Mass Spectrometry ◽  
Entire Group ◽  
Insulin Deficiency

We tested the generally accepted concept that increased gluconeogenesis (GNG) and endogenous glucose production (EGP) are the main reasons for postabsorptive hyperglycemia in patients with type 2 diabetes mellitus (T2DM). GNG was measured with the 2H2O method by use of both the C5-to-C2 ratio (C5/C2, with gas chromatography-mass spectrometry) and the C5-to-2H2O ratio (C5/2H2O, with isotope ratio mass spectrometry), and EGP was measured with 3-[3H]glucose in 27 patients with T2DM [13 with fasting plasma glucose (FPG) >10 mM and 14 with FPG <10 mM] and in 7 weight- and age-matched nondiabetic controls. The results showed 1) that GNG could be determined accurately with 2H2O by using either C5/C2 or C5/2H2O; 2) that whereas after an overnight fast of 16 h, GNG was higher in the entire group of patients with T2DM than in controls (6.4 vs. 5.0 μmol · kg−1 · min−1 or 60.4 vs. 51.4% of EGP, P < 0.02), GNG was within normal limits (less than the mean ± 2 SD of controls or <65.3%) in 11/14 (79%) patients with mild to moderate hyperglycemia (FPG <10 mM) and in 5/13 (38%) of patients with severe hyperglycemia (FPG 10–20 mM); 3) that elevated GNG in T2DM was associated with a 43% decrease in prehepatic insulin secretion, i.e., with hepatic insulin deficiency; and 4) that FPG correlated significantly with glucose clearance (insulin resistance) ( r = 0.70) and with GNG ( r = 0.50) or EGP ( r = 0.45). We conclude 1) that peripheral insulin resistance is at least as important as GNG (and EGP) as a cause of postabsorptive hyperglycemia in T2DM and 2) that GNG and EGP in T2DM are increased under conditions of significant hepatic insulin deficiency and thus probably represent a late event in the course of T2DM.

scholarly journals Ketone Bodies Are Mildly Elevated in Subjects with Type 2 Diabetes Mellitus and Are Inversely Associated with Insulin Resistance as Measured by the Lipoprotein Insulin Resistance Index

2020 ◽  
Vol 9 (2) ◽  
pp. 321 ◽  
Author(s):  
Erwin Garcia ◽  
Irina Shalaurova ◽  
Steven P. Matyus ◽  
David N. Oskardmay ◽  
James D. Otvos ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Mass Spectrometry ◽  
Insulin Resistance ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Ketone Bodies ◽  
Linear Regression Analysis ◽  
Resistance Index ◽  
Insulin Resistance Index

Background: Quantifying mildly elevated ketone bodies is clinically and pathophysiologically relevant, especially in the context of disease states as well as for monitoring of various diets and exercise regimens. As an alternative assay for measuring ketone bodies in the clinical laboratory, a nuclear magnetic resonance (NMR) spectroscopy-based test was developed for quantification of β-hydroxybutyrate (β-HB), acetoacetate (AcAc) and acetone. Methods: The ketone body assay was evaluated for precision, linearity and stability and method comparisons were performed. In addition, plasma ketone bodies were measured in the Insulin Resistance Atherosclerosis Study (IRAS, n = 1198; 373 type 2 diabetes mellitus (T2DM) subjects). Results: β-HB and AcAc quantified using NMR and mass spectrometry and acetone quantified using NMR and gas chromatography/mass spectrometry were highly correlated (R2 = 0.996, 0.994, and 0.994 for β-HB, AcAc, acetone, respectively). Coefficients of variation (%CVs) for intra- and inter-assay precision ranged from 1.3% to 9.3%, 3.1% to 7.7%, and 3.8% to 9.1%, for β-HB, AcAc and acetone, respectively. In the IRAS, ketone bodies were elevated in subjects with T2DM versus non-diabetic individuals (p = 0.011 to ≤0.001). Age- and sex-adjusted multivariable linear regression analysis revealed that total ketone bodies and β-HB were associated directly with free fatty acids (FFAs) and T2DM and inversely with triglycerides and insulin resistance as measured by the Lipoprotein Insulin Resistance Index. Conclusions: Concentrations of the three main ketone bodies can be determined by NMR with good clinical performance, are elevated in T2DM and are inversely associated with triglycerides and insulin resistance.

scholarly journals Effect of Empagliflozin on the Metabolic Signature of Patients With Type 2 Diabetes Mellitus and Cardiovascular Disease

Circulation ◽  
2017 ◽  
Vol 136 (10) ◽  
pp. 969-972 ◽  
Author(s):  
Ben A. Kappel ◽  
Michael Lehrke ◽  
Katharina Schütt ◽  
Anna Artati ◽  
Jerzy Adamski ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Cardiovascular Disease ◽  
Type 2 Diabetes Mellitus ◽  
Metabolic Signature
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