scholarly journals Protein Synthesis Inhibitors, in Synergy with 5-Azacytidine, Restore Sodium/Iodide Symporter Gene Expression in Human Thyroid Adenoma Cell Line, KAK-1, SuggestingTrans-Active Transcriptional Repressor

2007 ◽  
Vol 92 (3) ◽  
pp. 1080-1087 ◽  
Author(s):  
Wei Li ◽  
Gopalakrishnan M. Venkataraman ◽  
Kenneth B. Ain
Keyword(s):  
Gene Expression ◽  
Protein Synthesis ◽  
Cell Line ◽  
Sodium Iodide ◽  
Transcriptional Repressor ◽  
Human Thyroid ◽  
Thyroid Adenoma ◽  
Sodium Iodide Symporter ◽  
Protein Synthesis Inhibitors ◽  
Adenoma Cell

Reestablishment of In Vitro and In Vivo Iodide Uptake by Transfection of the Human Sodium Iodide Symporter (hNIS) in a hNIS Defective Human Thyroid Carcinoma Cell Line

Thyroid ◽  
2000 ◽  
Vol 10 (11) ◽  
pp. 939-943 ◽  
Author(s):  
Jan W.A. Smit ◽  
Janny P. Schröder-van der Elst ◽  
Marcel Karperien ◽  
Ivo Que ◽  
Gabri van der Pluijm ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Line ◽  
Sodium Iodide ◽  
Carcinoma Cell ◽  
Human Thyroid ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake ◽  
Thyroid Carcinoma Cell

scholarly journals Regulation by Human Chorionic Gonadotropin of Sodium/Iodide Symporter Gene Expression in the JAr Human Choriocarcinoma Cell Line

Endocrinology ◽  
2002 ◽  
Vol 143 (6) ◽  
pp. 2216-2220 ◽  
Author(s):  
Franco Arturi ◽  
Ludovic Lacroix ◽  
Ivan Presta ◽  
Daniela Scarpelli ◽  
Bernard Caillou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Choriocarcinoma Cell Line

scholarly journals In vivo long-term imaging and radioiodine therapy by sodium-iodide symporter gene expression using a lentiviral system containing ubiquitin C promoter

2007 ◽  
Vol 6 (7) ◽  
pp. 1130-1135 ◽  
Author(s):  
Hyun Joo Kim ◽  
Yong Hyun Jeon ◽  
Joo Hyun Kang ◽  
Yong Jin Lee ◽  
Kwang Kim ◽  
...  
Keyword(s):  
Gene Expression ◽  
Sodium Iodide ◽  
Radioiodine Therapy ◽  
Sodium Iodide Symporter

Detection of mRNA of sodium iodide symporter in benign and malignant human thyroid tissues

2000 ◽  
Vol 160 (1) ◽  
pp. 75-80 ◽  
Author(s):  
Miaw-Jene Liou ◽  
Jen-Der Lin ◽  
Err-Cheng Chan ◽  
Feng-Hsuan Liu ◽  
Tzu-Chieh Chao ◽  
...  
Keyword(s):  
Sodium Iodide ◽  
Human Thyroid ◽  
Sodium Iodide Symporter

scholarly journals Effect of Iodide on Human Choriogonadotropin, Sodium-Iodide Symporter Expression, and Iodide Uptake in BeWo Choriocarcinoma Cells

2007 ◽  
Vol 92 (10) ◽  
pp. 4046-4051 ◽  
Author(s):  
Huika Li ◽  
Kerry Richard ◽  
Brett McKinnon ◽  
Robin H. Mortimer
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Mrna Expression ◽  
Sodium Iodide ◽  
Sodium Iodide Symporter ◽  
Iodide Uptake ◽  
Hormone Synthesis ◽  
Human Choriogonadotropin ◽  
Fetal Thyroid ◽  
Choriocarcinoma Cells

Abstract Context: Active placental transport of maternal iodide by the thyroidal sodium iodide symporter (NIS) provides an essential substrate for fetal thyroid hormone synthesis. NIS is expressed in trophoblast and is regulated by human choriogonadotropin (hCG). In thyroid, iodide down-regulates expression of several genes including NIS. Placentas of iodine-deficient rats demonstrate up-regulation of NIS mRNA, suggesting a role for iodide in regulating placental NIS. Objectives and Methods: The objectives were to examine effects of iodide on expression of NIS and hCG in BeWo choriocarcinoma cells. Gene expression was studied by quantitative real-time PCR. Effects on NIS protein expression were assessed by Western blotting. Functional activity of NIS was measured by 125I uptake. Expression of hCG protein was assessed by immunoassay of secreted hormone. Results: Iodide inhibited NIS mRNA and membrane protein expression as well as 125I uptake, which were paralleled by decreased βhCG mRNA expression and protein secretion. Iodide had no effects on pendrin expression. Addition of hCG increased NIS mRNA expression. This effect was partially inhibited by addition of iodide. The inhibitory effects of iodide on NIS mRNA expression were abolished by propylthiouracil and dithiothreitol. Conclusions: We conclude that expression of placental NIS is modulated by maternal iodide. This may occur through modulation of hCG effects on NIS and hCG gene expression.

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