scholarly journals Kisspeptin in the hypothalamus of two rat models of polycystic ovary syndrome

Endocrinology ◽  
2016 ◽  
pp. en.2016-1333 ◽  
Author(s):  
Satoko Osuka ◽  
Akira Iwase ◽  
Tatsuo Nakahara ◽  
Mika Kondo ◽  
Ai Saito ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Rat Models

scholarly journals Effects of dodder total flavone on polycystic ovary syndrome rat models induced by DHEA combined HCG

2019 ◽  
Vol 26 (4) ◽  
pp. 821-827 ◽  
Author(s):  
Mingsan Miao ◽  
Mengfan Peng ◽  
Zhengwang Zhu ◽  
Xiaoli Yan ◽  
Zhenzhen Wei ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Ovary Syndrome ◽  
Rat Models

Astragalus hamosus Acts as an Insulin Sensitizer Through the Treatment of Polycystic Ovary Syndrome Rat Models by Affecting IRS1 Expression

2021 ◽  
Vol 21 ◽  
Author(s):  
Amir Nejati ◽  
Maryam Parvini Kohneh Shahri ◽  
Tarlan Farahvash
Keyword(s):  
Insulin Resistance ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Reproductive Age ◽  
Ovary Syndrome ◽  
Insulin Sensitizer ◽  
Rat Models ◽  
Qrt Pcr ◽  
Pcos Group ◽  
The Impact

Background: Polycystic ovary syndrome (PCOS) is the most common endocrine abnormality among women of reproductive age. Insulin resistance is known as the hallmark of PCOS that leads to hyperinsulinemia and type 2 diabetes in PCOS patients. Objective: This study aimed to evaluate the expression pattern of IRS1 as a candidate gene in insulin resistance development in the PCOS Rat models. Methods: In this study, estradiol valerate was used for PCOS induction. Then, all of the rats were divided into five experimental groups and treated with Astragalus hamosus extract. Ethanol was used for extraction by Soxhlet, and extracts were analyzed by GC-MS. Ovarian morphology was analyzed using histological experiments. Finally, the expression of IRS1 and hormonal titration of testosterone and insulin were evaluated using qRT-PCR and ELISA assays, respectively. Results: Induction of PCOS led to an increase in body weight, which decreased after treatment with the extract. Histological assessment declared an increased number of corpus luteums in treated groups and reduced cystic follicles compared with the PCOS group. Astragalus hamosus extract-treated groups exhibited decreased levels of insulin and testosterone compared to the PCOS group. qRT-PCR results showed an increase in the expression levels of IRS1 in the treated groups compared to the PCOS group. Conclusions: This study indicated the impact of Astragalus hamosus extract on PCOS by clarifying the increased levels of IRS1 expression in the treated groups compared to the PCOS group.

scholarly journals Expression of the anti-Mullerian hormone, kisspeptin 1, and kisspeptin 1 receptor in polycystic ovary syndrome and controlled ovarian stimulation rat models

2019 ◽  
Author(s):  
Ali Risvanli ◽  
Halis Ocal ◽  
Necati Timurkaan ◽  
Pınar Ipek ◽  
Ibrahim Seker ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Ovarian Stimulation ◽  
Polycystic Ovary ◽  
Controlled Ovarian Stimulation ◽  
Enzyme Linked Immunosorbent Assay ◽  
Histopathological Analysis ◽  
Ovary Syndrome ◽  
Rat Models ◽  
Immunohistochemical Assay ◽  
Estradiol Levels

Polycystic ovary syndrome represents a significant cause of female infertility. The aim of this study was to investigate the expression of anti-Mullerian hormone (AMH), kisspeptin 1 (KISS-1), and kisspeptin 1 receptor (KISS1r) in rat models of polycystic ovary syndrome (PCOS) and controlled ovarian stimulation (COS). For this purpose, 28 rats were assigned into four groups. Estrus and Diestrus groups consisted of rats in estrus and diestrus phases, respectively, while COS and PCOS groups consisted of rats with induced COS and PCOS, respectively. The serum AMH, KISS-1, and estradiol levels, and ovarian KISS1r levels were analyzed by enzyme-linked immunosorbent assay. Furthermore, histopathological analysis of the ovary tissue was done and ovarian KISS-1 expression was determined by immunohistochemical assay. The results revealed that ovarian KISS1r levels were higher in the Estrus (1271.43±51.98 pg/mL) and COS (1191.43±85.67 pg/mL) groups, compared to Diestrus and PCOS groups. The highest level of AMH was found in the Estrus group (16.91±2.12 ng/mL). The results indicate that AMH had no effect on the development of COS and PCOS, while  KISS-1 was found to affect the development of COS in rats.

scholarly journals How to choose the suitable animal model of polycystic ovary syndrome?

2018 ◽  
Vol 01 (02) ◽  
pp. 95-113 ◽  
Author(s):  
Amin Tamadon ◽  
Wei Hu ◽  
Peng Cui ◽  
Tong Ma ◽  
Xiaoyu Tong ◽  
...  
Keyword(s):  
Animal Model ◽  
Animal Models ◽  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Good Choice ◽  
Ovary Syndrome ◽  
Rat Models ◽  
Suitable Animal Model ◽  
Prenatal Androgen ◽  
Uncertain Etiology

Polycystic ovary syndrome (PCOS) is a gynecological metabolic and endocrine disorder with uncertain etiology. To understand the etiology of PCOS or the evaluation of various therapeutic agents, different animal models have been introduced. Considering this fact that is difficult to develop an animal model that mimics all aspects of this syndrome, but, similarity of biological, anatomical, and/or biochemical features of animal model to the human PCOS phenotypes can increase its application. This review paper evaluates the recently researched animal models and introduced the best models for different research purposes in PCOS studies. During January 2013 to January 2017, 162 studies were identified which applied various kinds of animal models of PCOS including rodent, primate, ruminant and fish. Between these models, prenatal and pre-pubertal androgen rat models and then prenatal androgen mouse model have been studied in detail than others. The comparison of main features of these models with women PCOS demonstrates higher similarity of these three models to human conditions. Thereafter, letrozole models can be recommended for the investigation of various aspects of PCOS. Interestingly, similarity of PCOS features of post-pubertal insulin and human chorionic gonadotropin rat models with women PCOS were considerable which can make it as a good choice for future investigations.

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