scholarly journals In Vitro Cultivation of AMF Using Root Organ Culture

2018 ◽  
pp. 95-108
Author(s):  
Sanjeev Kumar
Keyword(s):  
Organ Culture ◽  
In Vitro Cultivation ◽  
Root Organ Culture

scholarly journals A Protocol on in Vitro Propagation of Arbuscular Mycorrhizal Fungi using Root Organ Culture Technique

2017 ◽  
Vol 31 (1-2) ◽  
pp. 17-24
Author(s):  
Hari Prasad Aryal
Keyword(s):  
Arbuscular Mycorrhizal Fungi ◽  
Organ Culture ◽  
Mycorrhizal Fungi ◽  
In Vitro Propagation ◽  
Arbuscular Mycorrhizal ◽  
Culture Conditions ◽  
Culture Technique ◽  
The Past ◽  
Root Organ Culture

 The technique of in vitro propagation of Arbuscular mycorrhizal fungi has been developed over the past few decades and opens up areas of studying plant-fungi interactions. It is a scientific break through, especially for the study of the Arbuscular mycorrhizal fungi, since these obligate symbionts depend on host plant. The objective of this paper is to find out the in vitro culture of Arbuscular Mycorrhizal Fungi using Root Organ Culture technique. Ascertain of root colonization of these fungi could be affected in vitro without undertaking complex and complicated culture conditions. This could form an economically viable technique for root organ culture of Arbuscular mycorrhizal fungi.

IN VITRO CULTIVATION OF HUMAN ROTAVIRUS IN HUMAN FETAL GUT ORGAN CULTURE

The Lancet ◽  
1981 ◽  
Vol 318 (8254) ◽  
pp. 1053-1054
Author(s):  
C.W. Wells ◽  
R. Bura ◽  
R.R. Dourmashkin ◽  
T. Den ◽  
D.B. Sachar
Keyword(s):  
Organ Culture ◽  
In Vitro Cultivation ◽  
Human Rotavirus

scholarly journals Production of Arbuscular Mycorrhizal Fungi using In vitro Root Organ Culture and Phenolic Compounds

2019 ◽  
Vol 13 (4) ◽  
pp. 1985-1994
Author(s):  
Sawsan Abd Ellatif ◽  
Eman Abdullah M. Ali ◽  
Hoda H. Senousy ◽  
Elsayed S. Abdel Razik
Keyword(s):  
Phenolic Compounds ◽  
Arbuscular Mycorrhizal Fungi ◽  
Organ Culture ◽  
Mycorrhizal Fungi ◽  
Arbuscular Mycorrhizal ◽  
Root Organ Culture

Randomly amplified polymorphic DNA using the M13 core sequence of the vesicular–arbuscular mycorrhizal fungi Gigaspora margarita and Gigaspora gigantea

1997 ◽  
Vol 43 (8) ◽  
pp. 795-798 ◽  
Author(s):  
Vijay Gadkar ◽  
Alok Adholeya ◽  
T. Satyanarayana
Keyword(s):  
Organ Culture ◽  
Arbuscular Mycorrhizal ◽  
Gigaspora Margarita ◽  
Randomly Amplified Polymorphic Dna ◽  
Vam Fungi ◽  
Root Organ Culture ◽  
Vesicular Arbuscular ◽  
Fingerprint Pattern

Vesicular–arbuscular mycorrhizal (VAM) fungi are obligate symbionts, and a primary benefit provided to the host is the alleviation of stress. The recalcitrance of these fungi to grow in pure culture has spurred researchers to develop an alternative form of cultivation, namely the root organ culture (ROC). This synthetic form of production is new and efforts were made to use randomly amplified polymorphic DNA with the M13 minisatellite sequence as the polymerase chain reaction primer to look into polymorphism, if any, in the spores of Gigaspora margarita produced both in vitro and in situ (soil). The fingerprint patterns obtained from in vitro and in situ spores were similar. Extramatrical structures, such as auxiliary cells, were also examined by DNA fingerprinting. Their amplification pattern did not vary from the mother or daughter spores. A few interesting observations were made. For instance, the mother spore, which seemed hollow and inactive after germination, nevertheless contained nuclei after 4 months under in vitro conditions and generated a fingerprint pattern. The fingerprint pattern for Gigaspora margarita was different from that of Gigaspora gigantea, indicating that the minisatellite sequence could be exploited for identifying VAM fungi. ROC appears to be a truly representative system, in the sense that it mimics the essential features of the complex rhizosphere, allowing the fungi to complete their life cycle without any induced genetic changes per se. Key words : root organ culture, arbuscular mycorrhiza, M13 minisatellite sequence, randomly amplified polymorphic DNA.

IN VITRO METHODS FOR THE STUDY OF THE ADENOHYPOPHYSIAL FUNCTIONS TO SECRETE GONADOTROPHIN

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S27-S40 ◽  
Author(s):  
T. Kobayashi ◽  
T. Kigawa ◽  
M. Mizuno ◽  
T. Watanabe
Keyword(s):  
Cell Culture ◽  
Organ Culture ◽  
Cultured Cells ◽  
Cell Sheet ◽  
Short Term ◽  
Hypothalamic Extract ◽  
Numerous Cell ◽  
Single Cell Culture ◽  
Almost All

ABSTRACT There are several in vitro methods to analyse the function of the adenohypophysis or the mechanisms of its regulation. The present paper deals with single cell culture, organ culture and short term incubation techniques by which the morphology and gonadotrophin-secreting function of the adenohypophysis were studied. In trypsin-dispersed cell culture, the adenohypophysial cells showed extensive propagation to form numerous cell colonies and finally develop into a confluent monolayer cell sheet covering completely the surface of culture vessels. Almost all of the cultured cells, however, became chromophobic, at least at the end of the first week of cultivation, when gonadotrophin was detectable neither in the culture medium nor in the cells themselves. After the addition of the hypothalamic extract, gonadotrophin became detectable again, and basophilic or PAS-positive granules also reappeared within the cells, suggesting that the gonadotrophs were stimulated by the extract to produce gonadotrophin. In organ culture and short term incubation, the incorporation of [3H] leucine into the adenohypophysial cells in relation to the addition of hypothalamic extract was examined. It was obvious that the ability to incorporate [3H] leucine into the gonadotrophs in vitro was highly dependent upon the presence of the hypothalamic extract.

scholarly journals A prospectus of plant growth promoting endophytic bacterium from orchid (Vanda cristata)

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Sujit Shah ◽  
Krishna Chand ◽  
Bhagwan Rekadwad ◽  
Yogesh S. Shouche ◽  
Jyotsna Sharma ◽  
...  
Keyword(s):  
Plant Growth ◽  
Root Colonization ◽  
Endophytic Bacterium ◽  
Epifluorescence Microscopy ◽  
In Vitro Cultivation ◽  
Rrna Gene ◽  
Bacillus Spp ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background A plant growth-promoting endophytic bacterium PVL1 isolated from the leaf of Vanda cristata has the ability to colonize with roots of plants and protect the plant. PVL1 was isolated using laboratory synthetic media. 16S rRNA gene sequencing method has been employed for identification before and after root colonization ability. Results Original isolated and remunerated strain from colonized roots were identified as Bacillus spp. as per EzBiocloud database. The presence of bacteria in the root section of the plantlet was confirmed through Epifluorescence microscopy of colonized roots. The in-vitro plantlet colonized by PVL1 as well as DLMB attained higher growth than the control. PVL1 capable of producing plant beneficial phytohormone under in vitro cultivation. HPLC and GC-MS analysis suggest that colonized plants contain Indole Acetic Acid (IAA). The methanol extract of Bacillus spp., contains 0.015 μg in 1 μl concentration of IAA. PVL1 has the ability to produce antimicrobial compounds such as ethyl iso-allocholate, which exhibits immune restoring property. One-way ANOVA shows that results were statistically significant at P ≤ 0.05 level. Conclusions Hence, it has been concluded that Bacillus spp. PVL1 can promote plant growth through secretion of IAA during root colonization and ethyl iso-allocholate to protect plants from foreign infections. Thus, this study supports to support Koch’s postulates of bacteria establishment.

scholarly journals In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris
Keyword(s):  
Treponema Pallidum ◽  
In Vitro Cultivation
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