Preparation and characterization of an immunoaffinity column for the selective extraction of Ochratoxin A from cereal sample

2015 ◽  
pp. 423-426
Author(s):  
Hongxing Zhang ◽  
Yang Yang ◽  
Yuanhong Xie ◽  
Hui Liu ◽  
Baohua Kong ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Selective Extraction ◽  
Immunoaffinity Column

Preparation and characterization of an immunoaffinity column for the selective extraction of aflatoxin B1 in 13 kinds of foodstuffs

2015 ◽  
Vol 998-999 ◽  
pp. 50-56 ◽  
Author(s):  
Jie Xie ◽  
Tao Peng ◽  
Jian-Li He ◽  
Yu Shao ◽  
Chun-Lin Fan ◽  
...  
Keyword(s):  
Aflatoxin B1 ◽  
Selective Extraction ◽  
Immunoaffinity Column

Development and characterization of an immunoaffinity column for the selective extraction of bisphenol A from serum samples

2003 ◽  
Vol 783 (2) ◽  
pp. 401-410 ◽  
Author(s):  
Meiping Zhao ◽  
Yang Liu ◽  
Yuanzong Li ◽  
Xinxiang Zhang ◽  
Wenbao Chang
Keyword(s):  
Bisphenol A ◽  
Selective Extraction ◽  
Immunoaffinity Column ◽  
Serum Samples

scholarly journals Preparation and Characterization of an Immunoaffinity Column for the Selective Extraction of Salbutamol from Pork Sample

2011 ◽  
Vol 49 (4) ◽  
pp. 276-280 ◽  
Author(s):  
G. Wang ◽  
Y. Li ◽  
X. Li ◽  
X. Wang ◽  
Q. Guo ◽  
...  
Keyword(s):  
Selective Extraction ◽  
Immunoaffinity Column

Determination of Ochratoxin A in Feed by Immunoaffinity Cleanup and Liquid Chromatography

2013 ◽  
Vol 96 (3) ◽  
pp. 599-602 ◽  
Author(s):  
Ping Ding ◽  
Ziyou Mi ◽  
Yali Hou ◽  
Yigang He ◽  
Jianhua Xie
Keyword(s):  
Liquid Chromatography ◽  
Detection Limit ◽  
Ochratoxin A ◽  
Cyanogen Bromide ◽  
Polyclonal Antibodies ◽  
Immunoaffinity Column ◽  
Low Levels ◽  
Sepharose 4B ◽  
Feed Samples

Abstract A method using LC was developed for determination of ochratoxin A (OTA) in feeds. The extracted samples were cleaned up by an immunoaffinity column prepared by covalently coupling polyclonal antibodies against OTA to cyanogen bromide-activated Sepharose 4B. The eluates were determined by LC with fluorescence detection. Recoveries of OTA from fortified samples of 1–10 μg/kg levels ranged from 84.3 to 90.0%, with CVs of 3.3–7.8%. The detection limit was 0.045 μg/kg based on an S/N of 3:1. A total of 65 feed samples were screened for OTA with the proposed method. The results showed that only nine samples were contaminated with OTAs at low levels. The presented method was successfully applied to quantify OTAs in real feed samples.

scholarly journals Development and characterization of an immunoaffinity column for the detection of danofloxacin residues in milk samples

2019 ◽  
Vol 39 (2) ◽  
pp. 500-506 ◽  
Author(s):  
Rui NIU ◽  
Yamei YANG ◽  
Yadan WANG ◽  
Shilong LUO ◽  
Cheng ZHANG ◽  
...  
Keyword(s):  
Immunoaffinity Column ◽  
Milk Samples

Identification and characterization of the polyketide synthase involved in ochratoxin A biosynthesis in Aspergillus carbonarius

2014 ◽  
Vol 179 ◽  
pp. 10-17 ◽  
Author(s):  
Antonia Gallo ◽  
Benjamin P. Knox ◽  
Kenneth S. Bruno ◽  
Michele Solfrizzo ◽  
Scott E. Baker ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Polyketide Synthase ◽  
Aspergillus Carbonarius ◽  
Identification And Characterization

scholarly journals Comparison of two sample preparation procedures for HPLC determination of ochratoxin A

2009 ◽  
Vol 61 (4) ◽  
pp. 639-644 ◽  
Author(s):  
Gorica Vukovic ◽  
Snezana Pavlovic ◽  
M.S. Ristic
Keyword(s):  
Sample Preparation ◽  
Phosphoric Acid ◽  
Ochratoxin A ◽  
Chromatographic Determination ◽  
Liquid Extraction ◽  
Immunoaffinity Column ◽  
Maize Flour ◽  
Liquid Liquid Extraction ◽  
Immunoaffinity Columns

In preparation of samples for chromatographic determination of ochratoxin A, two types of columns were used for sample cleanup (SPE and immunoaffinity columns). The first method consisted of liquid-liquid extraction with a mixture of chloroform and phosphoric acid, followed by ion-exchange cleanup on Waters Oasis MAX columns. The sec?ond method consisted of extraction with a mixture of water and methanol, followed by LCTech OtaCLEAN immunoaf?finity column cleanup. Recoveries of the methods were determined at three levels in three repetitions for maize flour, and they were 84% (%RSD = 19.2) for the first method of sample preparation and 101% (%RSD = 2.2) for the second method. Values of LOQ for OTA were 0.25 and 1.00 ?g/kg for the IAC and SPE clean-up procedures, respectively. Both methods comply with present regulations, but the MAX sample clean-up procedure should be used as an alternative, since the immunoaffinity column clean-up procedure is characterized by better reproducibility, accuracy, and efficiency.

Synthesis and characterization of molecularly imprinted polymers with modified rosin as a cross-linker and selective SPE-HPLC detection of basic orange II in foods

2014 ◽  
Vol 6 (16) ◽  
pp. 6397-6406 ◽  
Author(s):  
Xiaoyan Li ◽  
Mei Li ◽  
Junjie Li ◽  
Fuhou Lei ◽  
Xiaomeng Su ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Selective Extraction ◽  
Synthesis And Characterization ◽  
Orange Ii ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Cross Linker

A novel sample clean-up technique, i.e., molecularly imprinted solid-phase extraction (MISPE) combined with HPLC, was developed and validated for the selective extraction and determination of basic orange II in foods.

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