scholarly journals Generating Phenotypic Diversity in a Fungal Biocatalyst to Investigate Alcohol Stress Tolerance Encountered during Microbial Cellulosic Biofuel Production

2015 ◽  
pp. 319-342
Keyword(s):  
Stress Tolerance ◽  
Phenotypic Diversity ◽  
Biofuel Production ◽  
Cellulosic Biofuel

scholarly journals Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Shannon M. Hoffman ◽  
Maria Alvarez ◽  
Gilad Alfassi ◽  
Dmitry M. Rein ◽  
Sergio Garcia-Echauri ◽  
...  
Keyword(s):  
Simultaneous Saccharification And Fermentation ◽  
Agricultural Waste ◽  
Biofuel Production ◽  
Cellulosic Biofuels ◽  
Processing Times ◽  
Cellulosic Biofuel ◽  
Thermotolerant Yeasts ◽  
Rapid Hydrolysis ◽  
Dedicated Energy Crops ◽  
Simultaneous Saccharification

Abstract Background Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between S. cerevisiae, O. polymorpha, and K. marxianus in glucose and cellulosic media at different temperatures. Results In this work, we show that cellulosic emulsions hydrolyze rapidly at temperatures tolerable to yeast, reaching up to 40-fold higher conversion in the first hour compared to microcrystalline cellulose (MCC). To evaluate suitable conditions for the eSSF process, we explored the upper temperature limits for the thermotolerant yeasts Kluyveromyces marxianus and Ogataea polymorpha, as well as Saccharomyces cerevisiae, and observed robust fermentation at up to 46, 50, and 42 °C for each yeast, respectively. We show that the eSSF process reaches high ethanol titers in short processing times, and produces close to theoretical yields at temperatures as low as 30 °C. Finally, we demonstrate the transferability of the eSSF technology to other products by producing the advanced biofuel isobutanol in a light-controlled eSSF using optogenetic regulators, resulting in up to fourfold higher titers relative to MCC SSF. Conclusions The eSSF process addresses the main challenges of cellulosic biofuel production by increasing saccharification rate at temperatures tolerable to yeast. The rapid hydrolysis of these emulsions at low temperatures permits fermentation using non-thermotolerant yeasts, short processing times, low enzyme loads, and makes it possible to extend the process to chemicals other than ethanol, such as isobutanol. This transferability establishes the eSSF process as a platform for the sustainable production of biofuels and chemicals as a whole.

scholarly journals Engineering of a novel cellulose-adherent cellulolytic Saccharomyces cerevisiae for cellulosic biofuel production

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Zhuo Liu ◽  
Shih-Hsin Ho ◽  
Kengo Sasaki ◽  
Riaan den Haan ◽  
Kentaro Inokuma ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Biofuel Production ◽  
Cellulosic Biofuel

scholarly journals Hybridization and adaptive evolution of diverse Saccharomyces species for cellulosic biofuel production

2017 ◽  
Vol 10 (1) ◽  
Author(s):  
David Peris ◽  
Ryan V. Moriarty ◽  
William G. Alexander ◽  
EmilyClare Baker ◽  
Kayla Sylvester ◽  
...  
Keyword(s):  
Adaptive Evolution ◽  
Biofuel Production ◽  
Cellulosic Biofuel

scholarly journals Advanced Rhodococcus Biocatalysts for Environmental Biotechnologies

Catalysts ◽  
2019 ◽  
Vol 9 (3) ◽  
pp. 236 ◽  
Author(s):  
Anastasiia Krivoruchko ◽  
Maria Kuyukina ◽  
Irena Ivshina
Keyword(s):  
Wastewater Treatment ◽  
Stress Tolerance ◽  
Process Parameters ◽  
Cell Immobilization ◽  
Biofuel Production ◽  
Environment Friendly ◽  
Efficient Approach ◽  
Catalytic Activities ◽  
Optimization Of Process Parameters

The review is devoted to biocatalysts based on actinobacteria of the genus Rhodococcus, which are promising for environmental biotechnologies. In the review, biotechnological advantages of Rhodococcus bacteria are evaluated, approaches used to develop robust and efficient biocatalysts are discussed, and their relevant applications are given. We focus on Rhodococcus cell immobilization in detail (methods of immobilization, criteria for strains and carriers, and optimization of process parameters) as the most efficient approach for stabilizing biocatalysts. It is shown that advanced Rhodococcus biocatalysts with improved working characteristics, enhanced stress tolerance, high catalytic activities, human and environment friendly, and commercially viable are developed, which are suitable for wastewater treatment, bioremediation, and biofuel production.

scholarly journals The potential of C4 grasses for cellulosic biofuel production

2013 ◽  
Vol 4 ◽  
Author(s):  
Tim van der Weijde ◽  
Claire L. Alvim Kamei ◽  
Andres F. Torres ◽  
Wilfred Vermerris ◽  
Oene Dolstra ◽  
...  
Keyword(s):  
Biofuel Production ◽  
C4 Grasses ◽  
Cellulosic Biofuel

scholarly journals Strategies for near-term scale-up of cellulosic biofuel production using sorghum and crop residues in the US

2018 ◽  
Vol 13 (12) ◽  
pp. 124002 ◽  
Author(s):  
Xinguang Cui ◽  
Olga Kavvada ◽  
Tyler Huntington ◽  
Corinne D Scown
Keyword(s):  
Crop Residues ◽  
Scale Up ◽  
Biofuel Production ◽  
Cellulosic Biofuel ◽  
The Us ◽  
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