Selection of the Type of Mobile Phases for Analysis of Nonionic Analytes: Reversed- and Normal-Phase HPLC

2015 ◽  
pp. 167-190
Author(s):  
Tomasz Tuzimski
Keyword(s):  
Normal Phase ◽  
Mobile Phases ◽  
Normal Phase Hplc ◽  
Selection Of

Normal phase HPLC profiling of the acetylcholinesterase activity in apolar plant extracts

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
A Landreau ◽  
S Bertrand ◽  
C Simoes-Pires ◽  
L Marcourt ◽  
TD Bach ◽  
...  
Keyword(s):  
Plant Extracts ◽  
Acetylcholinesterase Activity ◽  
Normal Phase ◽  
Normal Phase Hplc

Enantioseparation of novel chiral tetrahedral clusters on an amylose tris-(3,5-dimethylphenylcarbamate) chiral stationary phase by normal phase HPLC

2010 ◽  
Vol 22 (10) ◽  
pp. 1070-1074 ◽  
Author(s):  
Wen-Zhi Li ◽  
Xia Wang ◽  
Wei-Qiang Zhang ◽  
Chen Li-Ren ◽  
Yong-Min Li ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Chiral Stationary Phase ◽  
Normal Phase ◽  
Normal Phase Hplc

Diacylglycerols interfere in normal phase HPLC analysis of lipoxygenase products of docosahexaenoic or arachidonic acids

1986 ◽  
Vol 32 (6) ◽  
pp. 813-827 ◽  
Author(s):  
Magda Claeys ◽  
Haydee E.P. Bazan ◽  
Dale L. Birkle ◽  
Nicolas G. Bazan
Keyword(s):  
Hplc Analysis ◽  
Normal Phase ◽  
Lipoxygenase Products ◽  
Normal Phase Hplc ◽  
Arachidonic Acids

Extraction, Cleanup, and Quantitative Determination of Aflatoxins in Corn

1980 ◽  
Vol 63 (3) ◽  
pp. 631-633 ◽  
Author(s):  
James E Thean ◽  
David R Lorenz ◽  
David M Wilson ◽  
Kathleen Rodgers ◽  
Richard C Gueldner
Keyword(s):  
High Pressure ◽  
Liquid Chromatography ◽  
Quantitative Determination ◽  
Ammonium Sulfate ◽  
Silica Gel ◽  
Normal Phase ◽  
Aqueous Methanol ◽  
Normal Phase Hplc ◽  
Water Saturated

Abstract A method is proposed for extraction and cleanup of corn samples for the quantitation of 4 aflatoxins by high pressure liquid chromatography (HPLC). After aqueous methanol extraction, ammonium sulfate treatment, and partition of aflatoxins into chloroform, sample extracts are partially purified on Sep-Pak cartridges or small columns packed with HPLC grade silica; cleanup requires only 13 mL solvent/sample. Aflatoxins B1, B2, G1, and G2 in the purified extract are resolved in ca 10 min by normal phase HPLC on a microparticulate (5 μm) silica gel column with a 50% water-saturated chloroform-cyclohexaneacetonitrile- ethanol solvent, and are measured by ultraviolet fluorescence in a silica gel-packed flowcell. Recoveries of added aflatoxins B1, B2, G1, and G2 were 84–118 % at levels of 1.5–125 μg/kg

scholarly journals Detection of Methamphetamine, Methylenedioxymethamphetamine, and 3,4-Methylenedioxy-N-ethylamphetamine in Spiked Plasma by HPLC and TLC

2010 ◽  
Vol 93 (2) ◽  
pp. 556-561 ◽  
Author(s):  
Aysel Öztunç ◽  
Armağan Önal ◽  
Sidika Ertürk Toker
Keyword(s):  
Petroleum Ether ◽  
Silica Gel ◽  
Methyl Ketone ◽  
Aliphatic Amine ◽  
Normal Phase ◽  
Plasma Samples ◽  
Displacement Reaction ◽  
Mobile Phases ◽  
Spiked Plasma ◽  
Good Detection

Abstract HPLC and TLC methods were developed for separation and detection of some amphetamine analogs: methamphetamine (MA); 3,4-methylenedioxymethamphetamine (MDMA, ecstasy); and 3,4-methylenedioxy-N-ethylamphetamine (MDEA) in spiked plasma samples. The methods are based on purple chromogens formed by displacement reaction of these secondary aliphatic amine-bearing drugs with 7,7,8,8-tetracyanoquinodimethane at 80C for 25 min. For HPLC, both normal phase (silica gel) and RP (C18) columns were used. With the former, good detection limits in plasma were obtained with a 6 min run: 70, 100, and 500 ng/mL for MDMA, MA, and MDEA, respectively. For TLC, hexanechloroform (1 + 9) and benzene-diethyl ether-petroleum ether (4060)acetonitrileethyl methyl ketone (2 + 3.5 + 3.5 + 0.5 + 0.5) were used as mobile phases for silica gel 60 TLC and cyano-bonded silica gel HPTLC plates, respectively. The former offered more sensitive results than the latter. Influence of evaporation steps on recovery and interferences for the HPLC and TLC methods were investigated. The developed methods are selective, simple, and easily applicable.

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