Electrical Cell-substrate Impedance Spectroscopy (ECIS) measurements based on Oscillation-Based Test techniques

2015 ◽  
pp. 109-118
Author(s):  
Xudong Zhang ◽  
Remi Petrissans ◽  
Fang Li ◽  
Ioana Voiculescu

In this paper, an impedance spectroscopy biosensor fabricated on a stretchable substrate has been investigated. A thin stretchable membrane integrated with an impedance biosensor tolerated cyclic strain without cracking. The electric cell-substrate impedance spectroscopy (ECIS) technique has been used to monitor the impedance of the membrane of bovine aortic endothelial cells (BAEC). Integrating mechanical stretching and ECIS sensing onto a single platform requires biocompatible, highly flexible, and reversibly stretchable (elastic) materials for device fabrication. In addition, the interfacial impedance of electrode material to electrolyte should be small to improve the sensitivity of the impedance measurements. In this research, the biocompatible and stretchable membrane was fabricated from polydimethylsiloxane (PDMS), and the ECIS sensor was fabricated on pre-stretched PDMS membrane using sputtering microfabrication and pattern-transferring processes. The stretchable membrane, integrated with the ECIS sensor, can be used to simulate the dynamic environment of organisms and enable the analysis of the cell activity in vitro.


2020 ◽  
Vol 22 (2) ◽  
Author(s):  
Thomas Schmiedinger ◽  
Stefan Partel ◽  
Thomas Lechleitner ◽  
Oliver Eiter ◽  
Daniel Hekl ◽  
...  

2016 ◽  
Vol 6 (2) ◽  
pp. 502-511 ◽  
Author(s):  
Rachel C. Nordberg ◽  
Jianlei Zhang ◽  
Emily H. Griffith ◽  
Matthew W. Frank ◽  
Binil Starly ◽  
...  

2019 ◽  
Vol 31 (5) ◽  
pp. 1609
Author(s):  
Jong-Dae Kim ◽  
Chan-Young Park ◽  
Sang-Oh Park ◽  
Hye-Jeong Song ◽  
Yu-Seop Kim ◽  
...  

2015 ◽  
Vol 12 (2) ◽  
pp. 13
Author(s):  
Muhamad Faridz Osman ◽  
Karimah Kassim

The coordination complexes of Co(II) and Zn(II) with Schiff bases derived from o-phenylenediamine and substituted 2-hydroxybenzaldehyde were prepared All compounds were characterized by Fourier transform infrared (FTIR) spectroscopy and Nuclear magnetic resonance (NMR) spectroscopy elemental analyzers. They were analyzed using impedance spectroscopy in the frequency range of 100Hz-1 MHz. LI and L2 showed higher conductivity compared to their metal complexes, which had values of 1.3 7 x 10-7 and 6.13 x 10-8 S/cm respectively. 


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