Real-time sensing of epithelial cell-cell and cell-substrate interactions by impedance spectroscopy on porous substrates

2015 ◽  
Vol 118 (4) ◽  
pp. 044701 ◽  
Author(s):  
D. Mondal ◽  
D. Pal ◽  
C. RoyChaudhuri
Keyword(s):  
Epithelial Cell ◽  
Impedance Spectroscopy ◽  
Real Time ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Cell Cell ◽  
Porous Substrates

scholarly journals Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy

2010 ◽  
Vol 99 (12) ◽  
pp. 4028-4036 ◽  
Author(s):  
Victor Yashunsky ◽  
Vladislav Lirtsman ◽  
Michael Golosovsky ◽  
Dan Davidov ◽  
Benjamin Aroeti
Keyword(s):  
Epithelial Cell ◽  
Real Time ◽  
Surface Plasmon ◽  
Real Time Monitoring ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Surface Plasmon Spectroscopy ◽  
Cell Cell

scholarly journals NCAM polysialic acid can regulate both cell-cell and cell-substrate interactions.

1991 ◽  
Vol 114 (1) ◽  
pp. 143-153 ◽  
Author(s):  
A Acheson ◽  
J L Sunshine ◽  
U Rutishauser
Keyword(s):  
Tissue Culture ◽  
Cell Attachment ◽  
Surface Membrane ◽  
Polysialic Acid ◽  
Cell Aggregation ◽  
Important Variable ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Culture Models ◽  
Cell Cell

We have proposed previously that the polysialic acid (PSA) moiety of NCAM can influence membrane-membrane apposition, and thereby serve as a selective regulator of a variety of contact-dependent cell interactions. In this study, cell and tissue culture models are used to obtain direct evidence that the presence of PSA on the surface membrane can affect both cell-cell and cell-substrate interactions. Using a neuroblastoma/sensory neuron cell hybrid, it was found that removal of PSA with a specific neuraminidase (endo-N) augments cell-cell aggregation mediated by the L1 cell adhesion molecule as well as cell attachment to a variety of tissue culture substrates. In studies of embryonic spinal cord axon bundling, which involves both cell-cell and cell-substrate interactions, the pronounced defasciculation produced by removal of PSA is most easily explained by an increase in cell-substrate interaction. The fact that in both studies NCAM's intrinsic adhesion function was found not to be an important variable further illustrates that regulation of the cell surface by PSA can extend beyond binding mediated by the NCAM polypeptide.

scholarly journals Contact inhibition of locomotion and mechanical cross-talk between cell–cell and cell–substrate adhesion determine the pattern of junctional tension in epithelial cell aggregates

2016 ◽  
Vol 27 (22) ◽  
pp. 3436-3448 ◽  
Author(s):  
Luke Coburn ◽  
Hender Lopez ◽  
Benjamin J. Caldwell ◽  
Elliott Moussa ◽  
Chloe Yap ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cross Talk ◽  
Contact Inhibition ◽  
Cell Junctions ◽  
Asymmetric Distribution ◽  
Cell Aggregates ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Cell Substrate Adhesion ◽  
Cell Cell

We used a computational approach to analyze the biomechanics of epithelial cell aggregates—islands, stripes, or entire monolayers—that combines both vertex and contact-inhibition-of-locomotion models to include cell–cell and cell–substrate adhesion. Examination of the distribution of cell protrusions (adhesion to the substrate) in the model predicted high-order profiles of cell organization that agree with those previously seen experimentally. Cells acquired an asymmetric distribution of basal protrusions, traction forces, and apical aspect ratios that decreased when moving from the edge to the island center. Our in silico analysis also showed that tension on cell–cell junctions and apical stress is not homogeneous across the island. Instead, these parameters are higher at the island center and scale up with island size, which we confirmed experimentally using laser ablation assays and immunofluorescence. Without formally being a three-dimensional model, our approach has the minimal elements necessary to reproduce the distribution of cellular forces and mechanical cross-talk, as well as the distribution of principal stress in cells within epithelial cell aggregates. By making experimentally testable predictions, our approach can aid in mechanical analysis of epithelial tissues, especially when local changes in cell–cell and/or cell–substrate adhesion drive collective cell behavior.

scholarly journals Biotin ligase tagging identifies proteins proximal to E-cadherin, including lipoma preferred partner, a regulator of epithelial cell–cell and cell–substrate adhesion

2013 ◽  
Vol 127 (4) ◽  
pp. 885-895 ◽  
Author(s):  
Christina M. Van Itallie ◽  
Amber Jean Tietgens ◽  
Angel Aponte ◽  
Karin Fredriksson ◽  
Alan S. Fanning ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Biotin Ligase ◽  
Substrate Adhesion ◽  
Cell Substrate ◽  
Cell Substrate Adhesion ◽  
E Cadherin ◽  
Cell Cell
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