Cell–Substrate Interactions

2015 ◽  
pp. 83-98
Author(s):  
Rebecca Urbano ◽  
Alisa Clyne
Keyword(s):  
Substrate Interactions ◽  
Cell Substrate

Production and Analysis of High Resolution Polymer Replicas of Fibrillar Collagen

1999 ◽  
Vol 5 (S2) ◽  
pp. 398-399
Author(s):  
P. Sims ◽  
B. Todd ◽  
S. Eppell ◽  
T. Li ◽  
K. Park ◽  
...  
Keyword(s):  
Cellular Response ◽  
Physical Nature ◽  
Cell Types ◽  
Artificial Substrates ◽  
Adherent Cell ◽  
Fibrillar Collagen ◽  
New Materials ◽  
Substrate Interactions ◽  
Number Of Factors ◽  
Cell Substrate

Adherent cells generally construct the immediate substrate upon which they reside. This may occur via synthesis and secretion of new materials and/or by rearrangement and modification of existing substrate. The response of adherent cell types to an existing substrate can be influenced by a number of factors which include both the chemical and physical nature of the substrate. Cell adhesion, proliferation, differentiation and death can all be substrate dependent. Much effort has been directed toward chemical modification of substrates to regulate one or more of the parameters noted above. A significant, but somewhat smaller, degree of attention has been paid to the effects of the topography and microtopography on the cell response to substrate materials. Studies to date strongly suggest the topography is a significant factor in cell-substrate interactions. As noted above, it is most probable that both the chemistry and the structure of a substrate simultaneously influence the cellular response. However we wished to determine, particularly for artificial substrates, the role which microtopography can play in cell-substrate interactions.

scholarly journals Real-Time Monitoring of Epithelial Cell-Cell and Cell-Substrate Interactions by Infrared Surface Plasmon Spectroscopy

2010 ◽  
Vol 99 (12) ◽  
pp. 4028-4036 ◽  
Author(s):  
Victor Yashunsky ◽  
Vladislav Lirtsman ◽  
Michael Golosovsky ◽  
Dan Davidov ◽  
Benjamin Aroeti
Keyword(s):  
Epithelial Cell ◽  
Real Time ◽  
Surface Plasmon ◽  
Real Time Monitoring ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Surface Plasmon Spectroscopy ◽  
Cell Cell

EFFECTS OF CRYSTAL SIZE AND ORIENTATION OF SUBSTRATES ON CELL ADHESION: IMPLICATION FOR MEDICAL IMPLANTS

2008 ◽  
Vol 22 (18n19) ◽  
pp. 3069-3081 ◽  
Author(s):  
SHAHAB FAGHIHI ◽  
HOJATOLLAH VALI ◽  
MARYAM TABRIZIAN
Keyword(s):  
Cellular Response ◽  
High Pressure Torsion ◽  
Control Cell ◽  
Cell Activity ◽  
Titanium Implants ◽  
Polycrystalline Materials ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
Substrate Engineering ◽  
And Control

The aim of this study is to investigate the effect of atomic structure of polycrystalline materials on cell-substrate interactions. Samples are prepared from rods and sheets of Ti -6 Al -4 V substrates with predominately two distinct crystallographic orientations as well as nano-structured and annealed titanium fabricated through high-pressure torsion and heat treatment processes. The degree of preosteoblast attachment and rate of growth, which are regulated through the activity and interaction of proteins present in the extracellular matrix, are notably increased on the nano-structured titanium and substrate having predominant [Formula: see text] orientation. The improved cell activity is attributed to the nano-structured feature of these substrates consisting of ultra-fine crystals (< 50 nm) and specific atomic order of [Formula: see text] substrate which provide higher degree of surface wettability. These findings demonstrate the advantages of nano-structured titanium over the conventional and coated titanium implants, as both mechanical properties and cellular response are improved. Furthermore, crystal orientation of the substrates can influence cell responses and, therefore, substrate engineering can be used to improve and control cell-substrate interactions.

Effects of Friction Coefficient and Receptor Number on Cell-Substrate Interactions During Migration

2010 ◽  
Author(s):  
Henry C. Wong ◽  
William C. Tang
Keyword(s):  
Cell Migration ◽  
Cancer Metastasis ◽  
Element Model ◽  
Biological Tissues ◽  
Substrate Interactions ◽  
Strain Behavior ◽  
Structural Support ◽  
Research Areas ◽  
Cell Substrate ◽  
A Cell

Biological tissues are composed of cells that adhere to the extracellular matrix (ECM) via cell-surface integrin receptors that bind to specific proteins, such as fibronectin, embedded in the matrix. In this manner, the ECM functions as a structural support for the attached cells, and mechanical forces are able to be transmitted from the cell to the ECM and vice versa [1]. Cell migration, a process that is highly dependent on these mechanical interactions, is important for many normal biological processes and diseases that occur in the human body, which include embryonic development, immune response, would healing, and cancer invasion [2]. Though many continuum models of cell migration have been proposed, there is still a need for a model that can be used to quantitatively understand the mechanical factors that can influence the movement of a cell on a substrate. This would be invaluable to the research areas of tissue engineering as well as cancer metastasis. We utilized a finite element model to elucidate the mechanism of cell-substrate interactions for a cell that consistently migrates in a single direction. Our model follows the approach taken by Gracheva and Othmer [2], but we extended their model to describe two-dimensional plane strain behavior.

scholarly journals Mechanotransduction of Neural Cells Through Cell–Substrate Interactions

2016 ◽  
Vol 22 (3) ◽  
pp. 173-182 ◽  
Author(s):  
Jessica M. Stukel ◽  
Rebecca Kuntz Willits
Keyword(s):  
Neural Cells ◽  
Substrate Interactions ◽  
Cell Substrate

scholarly journals Altered Cell-Substrate Behavior on Microporous Membranes is a Result of Disruption and Grip

2019 ◽  
Author(s):  
Zahra Allahyari ◽  
Shayan Gholizadeh ◽  
Henry H. Chung ◽  
Luis F. Delgadillo ◽  
Thomas R. Gaborski
Keyword(s):  
Cell Communication ◽  
Pore Wall ◽  
Size Exclusion ◽  
Common Mechanism ◽  
Porous Membranes ◽  
Migration Speed ◽  
Microporous Membranes ◽  
Substrate Interactions ◽  
Cell Substrate ◽  
And Migration

ABSTRACTPorous membranes are ubiquitous in cell co-culture and tissue-on-a-chip studies. These materials are predominantly chosen for their semi-permeable and size exclusion properties to restrict or permit transmigration and cell-cell communication. However, previous studies have shown pore size, spacing and orientation affect cell behavior including extracellular matrix production and migration. The mechanism behind this behavior is not fully understood. In this study, we fabricated micropatterned non-fouling polyethylene glycol (PEG) islands to mimic pores in order to decouple the effect of surface discontinuity from grip provided by pore wall edges. Similar to porous membranes, we found that the PEG islands hindered fibronectin fibrillogenesis with cells on patterned substrates producing shorter fibrils. Additionally, cell migration speed over micropatterned PEG islands was greater than unpatterned controls, suggesting that disruption of cell-substrate interactions by PEG islands promoted a more dynamic and migratory behavior, similarly to cells migrating on microporous membranes. Preferred cellular directionality during migration was nearly identical between substrates with identically patterned PEG islands and micropores, further confirming disruption of cell-substrate interactions as a common mechanism behind the cellular responses on these substrates. Interestingly, cell spreading and the magnitude of migration speed was significantly greater on porous membranes compared to PEG islands with identical feature size and spacing, suggesting pore edges enhanced cellular grip. These results provide a more complete picture on how porous membranes affect cells which are grown on them in an increasing number of cellular barrier and co-culture studies.

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