Microfabrication of Three-Dimensional Vascular Structures

2014 ◽  
pp. 162-181 ◽  
Keyword(s):  
Three Dimensional ◽  
Vascular Structures

scholarly journals Hematopoiesis in 3 dimensions: human and murine bone marrow architecture visualized by confocal microscopy

Blood ◽  
2010 ◽  
Vol 116 (15) ◽  
pp. e41-e55 ◽  
Author(s):  
Tomoiku Takaku ◽  
Daniela Malide ◽  
Jichun Chen ◽  
Rodrigo T. Calado ◽  
Sachiko Kajigaya ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Three Dimensional ◽  
Cell Types ◽  
Bone Marrow Tissue ◽  
Murine Bone Marrow ◽  
3 Dimensional ◽  
Vascular Structures ◽  
Bone Structures ◽  
Surrounding Bone

AbstractIn many animals, blood cell production occurs in the bone marrow. Hematopoiesis is complex, requiring self-renewing and pluripotent stem cells, differentiated progenitor and precursor cells, and supportive stroma, adipose tissue, vascular structures, and extracellular matrix. Although imaging is a vital tool in hematology research, the 3-dimensional architecture of the bone marrow tissue in situ remains largely uncharacterized. The major hindrance to imaging the intact marrow is the surrounding bone structures are almost impossible to cut/image through. We have overcome these obstacles and describe a method whereby whole-mounts of bone marrow tissue were immunostained and imaged in 3 dimensions by confocal fluorescence and reflection microscopy. We have successfully mapped by multicolor immunofluorescence the localization pattern of as many as 4 cell features simultaneously over large tiled views and to depths of approximately 150 μm. Three-dimensional images can be assessed qualitatively and quantitatively to appreciate the distribution of cell types and their interrelationships, with minimal perturbations of the tissue. We demonstrate its application to normal mouse and human marrow, to murine models of marrow failure, and to patients with aplastic anemia, myeloid, and lymphoid cell malignancies. The technique should be generally adaptable for basic laboratory investigation and for clinical diagnosis of hematologic diseases.

scholarly journals MACS: Rapid aqueous clearing system for three-dimensional mapping of intact organs

2019 ◽  
Author(s):  
Jingtan Zhu ◽  
Tingting Yu ◽  
Yusha Li ◽  
Jianyi Xu ◽  
Yisong Qi ◽  
...  
Keyword(s):  
Three Dimensional ◽  
High Concentration ◽  
Volume Imaging ◽  
Three Dimensional Mapping ◽  
Clearing System ◽  
Vascular Structures ◽  
Short Time ◽  
Tissue Optical Clearing ◽  
Clearing Method ◽  
Dimensional Mapping

Tissue optical clearing techniques have provided important tools for large-volume imaging. Aqueous-based clearing methods are known for good fluorescence preservation and scalable size maintenance, but are limited by either long incubation time, or insufficient clearing performance, or requirements for specialized devices. Additionally, due to the use of high concentration organic solvents or detergents, few clearing methods are compatible with lipophilic dyes while maintaining high clearing performance. To address these issues, we developed a rapid, highly efficient aqueous clearing method with robust compatibility, termed m-xylylenediamine (MXDA)-based Aqueous Clearing System (MACS). MACS can render intact organs highly transparent in a fairly short time and possesses ideal compatibility with multiple probes, especially for lipophilic dyes. Using MACS, we cleared the adult mouse brains within only 2.5 days for three-dimensional (3D) imaging of the neural structures labelled by various techniques. Combining MACS with DiI labelling, we visualized the vascular structures of various organs. MACS provides a useful tool for 3D mapping of intact tissues and is expected to facilitate morphological, physiological and pathological studies of various organs.

scholarly journals Three-Dimensional Visualization of Blood Flow in Vascular Structures

1994 ◽  
Vol 14 (53) ◽  
pp. 116-120_1
Author(s):  
Tad W. TAYLOR ◽  
Haruka OKINO ◽  
Takami YAMAGUCHI
Keyword(s):  
Blood Flow ◽  
Three Dimensional ◽  
Vascular Structures

scholarly journals Computer-assisted spatial reconstruction of cerebral blood vessels and intracranial aneurysms

2006 ◽  
Vol 59 (1-2) ◽  
pp. 24-27 ◽  
Author(s):  
Igor Nikolic
Keyword(s):  
Three Dimensional ◽  
Cerebral Aneurysms ◽  
Carotid Bifurcation ◽  
Computer Assisted ◽  
Three Dimensional Reconstruction ◽  
Conventional Angiography ◽  
Spatial Reconstruction ◽  
Dimensional Reconstruction ◽  
Vascular Structures ◽  
Carotid Aneurysms

Introduction. Today, many medical procedures are based on image analysis with the aim of providing accurate diagnosis and optimal treatment. The goal of this report was to present clinical implications of computer-assisted geometric design of carotid aneurysms. Material and methods. In this study, the three-dimensional reconstruction was based on the approximation power of the parametric spline function, which achieves interpolation and surface fitting of the arterial information obtained by conventional angiography. Two views of conventional angiograms (anterioposterior and lateral views) were used with a personal computer and commercial software. Results. This method of three-dimensional generated images was applied in 45 cases of cerebral aneurysms in carotid bifurcation. 3D reconstructions were made in approximately 20 minutes. They contributed to assessing vascular structures, and it was possible to rotate the three-dimensional image in different angles. Conclusion. Three-dimensional reconstruction of cerebral vessels is very useful for discussing surgical strategies preoperatively. Furthermore, it could also be used in endovascular procedures. .

scholarly journals Embossed Membranes with Vascular Patterns Guide Vascularization in a 3D Tissue Model

Polymers ◽  
2019 ◽  
Vol 11 (5) ◽  
pp. 792 ◽  
Author(s):  
Soyoung Hong ◽  
Eun Young Kang ◽  
Jaehee Byeon ◽  
Sung-ho Jung ◽  
Changmo Hwang
Keyword(s):  
Blood Vessel ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Electrospun Membrane ◽  
Tissue Constructs ◽  
Vacuum Forming ◽  
Electrospun Membranes ◽  
Vascular Structures ◽  
Endothelial Growth Factor ◽  
Flat Membranes

The vascularization of three-dimensional (3D) tissue constructs is necessary for transporting nutrients and oxygen to the component cells. In this study, a vacuum forming method was applied to emboss a vascular pattern on an electrospun membrane so that guided vascular structures could develop within the construct. Two- or six-layer constructs of electrospun membranes seeded with endothelial cells and pericytes were stacked and subcutaneously implanted into mice. Blood vessel formation in the implanted constructs with six alternating layers of flat membranes and membranes embossed with a blood vessel pattern was observed after two weeks of implantation. The formation of blood vessels was observed along the embossed blood vessel pattern in the structure of the embossed membrane laminated at four weeks and eight weeks. Vascular endothelial growth factor (VEGF) and angiopoietin 1 (Ang-1) were highly expressed in the vascularized structures. Therefore, we demonstrated that a structure capable of producing a desired blood vessel shape with electrospun membranes embossed with a blood vessel pattern can be manufactured, and that a variety of structures can be manufactured using electrospun membranes in the tissue engineering era.

scholarly journals 3D digital subtracted CT angiography to evaluate the venous anatomy in extra-axial tumors invading the major dural venous sinuses

2017 ◽  
Vol 23 (4) ◽  
pp. 346-349 ◽  
Author(s):  
Stéphanie Lenck ◽  
Damien Bresson ◽  
Anne-Laure Bernat ◽  
Jean-Pierre Saint-Maurice ◽  
Marc-Antoine Labeyrie ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Surgical Excision ◽  
Dural Sinus ◽  
Vascular Tumors ◽  
Accurate Assessment ◽  
Two Dimensional ◽  
Venous Anatomy ◽  
Surgical Guidance ◽  
Non Invasive ◽  
Vascular Structures

Background Investigation of the venous system surrounding a tumor that is invading a dural sinus is of great use for guiding the surgical excision. Non-invasive imaging is often inadequate since enhancement of the tumor causes it to blend with the contrast of the venous vascular structures. Conventional two-dimensional angiography is also often insufficient. Objective The objective of this study was to report regarding the potential of three-dimensional digital subtracted computed tomography angiography (3D DS-CTA) as a technique to preoperatively explore these tumors. Methods We retrospectively studied the radiological and surgical features of patients explored with 3D DS-CTA for a tumor invading a major dural sinus. Results Three patients were included in this study, one hemangiopericytoma and two meningiomas. 3D DS-CTA allows for accurate assessment of the patency of the sinus, the location of the secondary intra- and trans-osseous venous outlets, and surgical guidance by neuronavigation. Conclusion 3D DS-CTA could be a promising guiding and diagnostic tool for the pre- and intraoperative treatment of vascular tumors invading the dural sinuses, for which the venous morbidity and mortality is substantial.

Intravascular Ultrasound: The Ultimate Tool for Abdominal Aortic Aneurysm Assessment and Endovascular Graft Delivery

1997 ◽  
Vol 4 (1) ◽  
pp. 45-55 ◽  
Author(s):  
Rodney A. White ◽  
Carlos Donayre ◽  
George Kopchok ◽  
Irwin Walot ◽  
Eric Wilson ◽  
...  
Keyword(s):  
Intravascular Ultrasound ◽  
Three Dimensional ◽  
Aortic Aneurysms ◽  
Developmental Studies ◽  
Abdominal Aortic ◽  
Device Placement ◽  
Vascular Structures ◽  
Ivus Imaging ◽  
Aortic Morphology

Intravascular ultrasound (IVUS) imaging is a relatively new, rapidly evolving technology that enables precise catheter-based assessment of the dimensions and morphology of vascular structures and lesions. In extensive preclinical laboratory developmental studies and in clinical cases of endograft deployment for treatment of abdominal aortic aneurysms, we have found IVUS invaluable for determining key parameters of aortic morphology before and during interventions and for assessing the accuracy of deployment after device placement. By combining the IVUS data with information obtained from angiography, magnetic resonance imaging, and computed tomography (axial and three-dimensional reconstructions), we have been able to size devices and choose optimal fixation sites to prevent endoleaks and maintain luminal patency acutely and in the long term.

scholarly journals Lymphatic vessels in human adipose tissue

2019 ◽  
Vol 379 (3) ◽  
pp. 511-520 ◽  
Author(s):  
Patricia de Albuquerque Garcia Redondo ◽  
Fernanda Gubert ◽  
Camila Zaverucha-do-Valle ◽  
Tatiana Pereira Pena Dutra ◽  
Jackline de Paula Ayres-Silva ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Three Dimensional ◽  
Lymphatic Vessels ◽  
Human Adipose Tissue ◽  
Unanswered Question ◽  
Three Dimensional Imaging ◽  
Whole Mount ◽  
Lymphatic Network ◽  
Vascular Structures

AbstractDespite being considered present in most vascularised tissues, lymphatic vessels have not been properly shown in human adipose tissue (AT). Our goal in this study is to investigate an unanswered question in AT biology, regarding lymphatic network presence in tissue parenchyma. Using human subcutaneous (S-) and visceral (V-) AT samples with whole mount staining for lymphatic specific markers and three-dimensional imaging, we showed lymphatic capillaries and larger lymphatic vessels in the human VAT. Conversely, in the human SAT, microcirculatory lymphatic vascular structures were rarely detected and no initial lymphatics were found.

scholarly journals Label-free three-dimensional observations and quantitative characterisation of on-chip vasculogenesis using optical diffraction tomography

2020 ◽  
Author(s):  
Chungha Lee ◽  
Seunggyu Kim ◽  
Herve Hugonnet ◽  
Moosung Lee ◽  
Weisun Park ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Optical Diffraction ◽  
Diffraction Tomography ◽  
Label Free ◽  
Vascular Networks ◽  
Optical Diffraction Tomography ◽  
Quantitative Characterisation ◽  
Vascular Structures ◽  
On Chip ◽  
Intrinsic Imaging

Label-free, three-dimensional (3D) quantitative observations of on-chip vasculogenesis were achieved using optical diffraction tomography. Exploiting 3D refractive index maps as an intrinsic imaging contrast, the vascular structures, multicellular activities, and subcellular organelles of endothelial cells were imaged and analysed throughout vasculogenesis to characterise mature vascular networks without exogenous labelling.

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