Arctigenin from Arctium Lappa Inhibits Interleukin-2 and Interferon Gene Expression in Primary Human T Lymphocytes

2014 ◽  
pp. 145-162
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Interleukin 2 ◽  
Arctium Lappa ◽  
Human T Lymphocytes ◽  
Interferon Gene

scholarly journals Arctigenin from Arctium lappa inhibits interleukin-2 and interferon gene expression in primary human T lymphocytes

2011 ◽  
Vol 6 (1) ◽  
pp. 12 ◽  
Author(s):  
Wei-Jern Tsai ◽  
Chu-Ting Chang ◽  
Guei-Jane Wang ◽  
Tzong-Huei Lee ◽  
Shwu-Fen Chang ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Interleukin 2 ◽  
Arctium Lappa ◽  
Human T Lymphocytes ◽  
Interferon Gene

scholarly journals Two distinct mechanisms of interleukin-2 gene expression in human T lymphocytes

1989 ◽  
Vol 2 ◽  
pp. 55-65 ◽  
Author(s):  
Carl H. June ◽  
Kelly M. Jackson ◽  
Jeffrey A. Ledbetter ◽  
Jeffrey M. Leiden ◽  
Tullia Lindsten ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Interleukin 2 ◽  
Human T Lymphocytes

scholarly journals Interleukin 2 receptor gene expression in normal human T lymphocytes.

1985 ◽  
Vol 82 (18) ◽  
pp. 6281-6285 ◽  
Author(s):  
W. J. Leonard ◽  
M. Kronke ◽  
N. J. Peffer ◽  
J. M. Depper ◽  
W. C. Greene
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Receptor Gene ◽  
Interleukin 2 ◽  
Interleukin 2 Receptor ◽  
Human T Lymphocytes ◽  
Normal Human ◽  
Receptor Gene Expression

scholarly journals Two Distinct Mechanisms of Interleukin-2 Gene Expression in Human T Lymphocytes

1989 ◽  
pp. 55-65
Author(s):  
Carl H. June ◽  
Kelly M. Jackson ◽  
Jeffrey A. Ledbetter ◽  
Jeffrey M. Leiden ◽  
Tullia Lindsten ◽  
...  
Keyword(s):  
Gene Expression ◽  
T Lymphocytes ◽  
Interleukin 2 ◽  
Human T Lymphocytes

scholarly journals Involvement of P-glycoprotein in the transmembrane transport of interleukin-2 (IL-2), IL-4, and interferon-gamma in normal human T lymphocytes

Blood ◽  
1996 ◽  
Vol 88 (5) ◽  
pp. 1747-1754 ◽  
Author(s):  
J Drach ◽  
A Gsur ◽  
G Hamilton ◽  
S Zhao ◽  
J Angerler ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Interferon Gamma ◽  
T Cell Activation ◽  
Interleukin 2 ◽  
Rhodamine 123 ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ifn Gamma ◽  
Human T Lymphocytes ◽  
P Glycoprotein ◽  
Normal Human

Abstract The physiological role of the multidrug resistance P-glycoprotein (P- gp), which is expressed by normal human T lymphocytes, is still largely unknown. To investigate whether or not P-gp is involved in the transport of cytokines, peripheral blood lymphocytes were stimulated with phytohemagglutinin (PHA) in the absence or presence of P-gp inhibitors, and concentrations of cytokines (interleukin-2 [IL-2], IL- 4, IL-6, interferon-gamma [IFN-gamma]) in the supernatants of these cultures were quantitated by enzyme-linked immunosorbent assay. P-gp inhibitors included verapamil (Ver), tamoxifen (Tmx), and the P-gp specific monoclonal antibody UIC2. Release of IL-2 was significantly suppressed by these inhibitors at concentrations that were also effective in blocking efflux of Rhodamine-123 from normal T lymphocytes. IL-2 mRNA expression in lymphocytes was not different between PHA control and the cultures with P-gp inhibitors. Ver and Tmx did not interfere with T-cell activation as determined by CD25 and CD69 expression. In a nonhematological model, the P-gp expressing HCT-8 adenocarcinoma cell line, exogenously added IL-2 was shown to exert an inhibitory effect on P-gp mediated Rhodamine-123 efflux. In addition, transepithelial transport of IL-2 by electrophysiologically tight and polarized HCT-8 monolayers was examined. A time-dependent flux of IL-2 across dense monolayers, which was partially inhibited by Ver, was observed. We also investigated whether or not P-gp inhibitors suppressed release of other cytokines produced by activated T cells (IL- 4, IL-6, IFN-gamma). Release of IL-4 and IFN-gamma was significantly inhibited by Ver, Tmx, and UIC2; however, release of IL-6 remained unaffected. These data show P-gp mediated transmembrane flux of IL-2 in T lymphocytes and HCT-8 cells. We conclude that P-gp participates in the transport of cytokines (IL-2, IL-4, and IFN-gamma) in normal peripheral T lymphocytes.

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