Molecular Farming in the Decades of Omics

2013 ◽  
pp. 563-602
Author(s):  
Dinesh Yadav ◽  
PhD Yadav ◽  
S Khurana
Keyword(s):  
Molecular Farming

Developing Country Applications of Molecular Farming: Case Studies in South Africa and Argentina

2013 ◽  
Vol 19 (31) ◽  
pp. 5612-5621 ◽  
Author(s):  
Edward Rybicki ◽  
Inga Hitzeroth ◽  
Ann Meyers ◽  
Maria Santos ◽  
Andres Wigdorovitz
Keyword(s):  
South Africa ◽  
Case Studies ◽  
Developing Country ◽  
Molecular Farming

scholarly journals Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II

Foods ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 838
Author(s):  
Kirolos D. Kelada ◽  
Daniel Tusé ◽  
Yuri Gleba ◽  
Karen A. McDonald ◽  
Somen Nandi
Keyword(s):  
Economic Analysis ◽  
Process Simulation ◽  
Large Scale ◽  
Recombinant Expression ◽  
Molecular Farming ◽  
Consumer Preference ◽  
Production Method ◽  
Long Term Effects ◽  
Adverse Health Effects ◽  
Sweet Proteins

There are currently worldwide efforts to reduce sugar intake due to the various adverse health effects linked with the overconsumption of sugars. Artificial sweeteners have been used as an alternative to nutritive sugars in numerous applications; however, their long-term effects on human health remain controversial. This led to a shift in consumer preference towards non-caloric sweeteners from natural sources. Thaumatins are a class of intensely sweet proteins found in arils of the fruits of the West-African plant Thaumatococcus daniellii. Thaumatins’ current production method through aqueous extraction from this plant and uncertainty of the harvest from tropical rainforests limits its supply while the demand is increasing. Despite successful recombinant expression of the protein in several organisms, no large-scale bioproduction facilities exist. We present preliminary process design, process simulation, and economic analysis for a large-scale (50 metric tons/year) production of a thaumatin II variant using several different molecular farming platforms.

scholarly journals Tackling Unwanted Proteolysis in Plant Production Hosts Used for Molecular Farming

2016 ◽  
Vol 7 ◽  
Author(s):  
Manoj K. Mandal ◽  
Houtan Ahvari ◽  
Stefan Schillberg ◽  
Andreas Schiermeyer
Keyword(s):  
Molecular Farming ◽  
Plant Production

scholarly journals Peculiarities of the Transformation of Asteraceae Family Species: The Cases of Sunflower and Lettuce

2021 ◽  
Vol 12 ◽  
Author(s):  
Flavia Soledad Darqui ◽  
Laura Mabel Radonic ◽  
Valeria Cecilia Beracochea ◽  
H. Esteban Hopp ◽  
Marisa López Bilbao
Keyword(s):  
Genetic Transformation ◽  
Molecular Farming ◽  
Plant Biotechnology ◽  
Transformation Techniques ◽  
Single Compound ◽  
Complete Sequencing ◽  
Plant Families ◽  
Transformation Vectors ◽  
Asteraceae Family

The Asteraceae family is the largest and most diversified family of the Angiosperms, characterized by the presence of numerous clustered inflorescences, which have the appearance of a single compound flower. It is estimated that this family represents around 10% of all flowered species, with a great biodiversity, covering all environments on the planet, except Antarctica. Also, it includes economically important crops, such as lettuce, sunflower, and chrysanthemum; wild flowers; herbs, and several species that produce molecules with pharmacological properties. Nevertheless, the biotechnological improvement of this family is limited to a few species and their genetic transformation was achieved later than in other plant families. Lettuce (Lactuca sativa L.) is a model species in molecular biology and plant biotechnology that has easily adapted to tissue culture, with efficient shoot regeneration from different tissues, organs, cells, and protoplasts. Due to this plasticity, it was possible to obtain transgenic plants tolerant to biotic or abiotic stresses as well as for the production of commercially interesting molecules (molecular farming). These advances, together with the complete sequencing of lettuce genome allowed the rapid adoption of gene editing using the CRISPR system. On the other hand, sunflower (Helianthus annuus L.) is a species that for years was considered recalcitrant to in vitro culture. Although this difficulty was overcome and some publications were made on sunflower genetic transformation, until now there is no transgenic variety commercialized or authorized for cultivation. In this article, we review similarities (such as avoiding the utilization of the CaMV35S promoter in transformation vectors) and differences (such as transformation efficiency) in the state of the art of genetic transformation techniques performed in these two species.

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