scholarly journals Tackling Unwanted Proteolysis in Plant Production Hosts Used for Molecular Farming

2016 ◽  
Vol 7 ◽  
Author(s):  
Manoj K. Mandal ◽  
Houtan Ahvari ◽  
Stefan Schillberg ◽  
Andreas Schiermeyer
Keyword(s):  
Molecular Farming ◽  
Plant Production

scholarly journals Engineering Approaches in Plant Molecular Farming for Global Health

Vaccines ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 1270
Author(s):  
Advaita Acarya Singh ◽  
Priyen Pillay ◽  
Tsepo Lebiletsa Tsekoa
Keyword(s):  
Global Health ◽  
Posttranslational Modifications ◽  
Secretory Pathway ◽  
Molecular Farming ◽  
Plant Production ◽  
Functional Protein ◽  
Case Examples ◽  
Plant Molecular Farming ◽  
Complex Protein ◽  
Protein Vaccines

Since the demonstration of the first plant-produced proteins of medical interest, there has been significant growth and interest in the field of plant molecular farming, with plants now being considered a viable production platform for vaccines. Despite this interest and development by a few biopharmaceutical companies, plant molecular farming is yet to be embraced by ‘big pharma’. The plant system offers a faster alternative, which is a potentially more cost-effective and scalable platform for the mass production of highly complex protein vaccines, owing to the high degree of similarity between the plant and mammalian secretory pathway. Here, we identify and address bottlenecks in the use of plants for vaccine manufacturing and discuss engineering approaches that demonstrate both the utility and versatility of the plant production system as a viable biomanufacturing platform for global health. Strategies for improving the yields and quality of plant-produced vaccines, as well as the incorporation of authentic posttranslational modifications that are essential to the functionality of these highly complex protein vaccines, will also be discussed. Case-by-case examples are considered for improving the production of functional protein-based vaccines. The combination of all these strategies provides a basis for the use of cutting-edge genome editing technology to create a general plant chassis with reduced host cell proteins, which is optimised for high-level protein production of vaccines with the correct posttranslational modifications.

Irrigation Volume, Application, and Controlled-release Fertilizer II. Effect on Substrate Solution Nutrient Concentration and Water Efficiency in Containerized Plant Production

1998 ◽  
Vol 16 (3) ◽  
pp. 182-188
Author(s):  
Kelly M. Groves ◽  
Stuart L. Warren ◽  
Ted E. Bilderback
Keyword(s):  
Controlled Release ◽  
Irrigation Efficiency ◽  
Plant Production ◽  
Water Efficiency ◽  
Solution Ph ◽  
Entire Study ◽  
Once Daily ◽  
Substrate Solution ◽  
Irrigation Volume ◽  
Controlled Release Fertilizers

Abstract Rooted cuttings of Cotoneaster dammeri Schneid ‘Skogholm’ and seedlings of Rudbeckia fulgida Ait. ‘Goldsturm’ were potted into 3.8 liter (4 qt) containers in a pine bark:sand (8:1 by vol) substrate incorporated with 3.5 g (0.12 oz) N per container provided by one of the following five controlled-release fertilizers (CRFs): Meister 21N–3.5P–11.1K (21–7–14), Osmocote 24N–2.0P–5.6K (24–4–7), Scotts 23N–2.0P–6.4K (23–4–8), Sustane 5N–0.9P–3.3K (5–2–4) or Woodace 21N–3.0P–9.5K (21–6–12). Two hundred ml (0.3 in), 400 ml (0.6 in), 800 ml (1.1 in) or 1200 ml (1.7 in) of water was applied once daily (single) or in two equal applications with a 2 hr interval between applications (cyclic). Substrate solutions were collected from containers of cotoneaster 15, 32, 45, 60, 74, 90, 105, and 119 days after initiation (DAI). Irrigation efficiency [(water applied − water leached) ÷ water applied] was determined on the same days. Cyclic application improved irrigation efficiency at 800 ml (1.1 in) and 1200 ml (1.7 in) ≈ 27% compared to a single application. Irrigation efficiencies averaged over the season were 95%, 84%, 62%, and 48% for cotoneaster and 100%, 90%, 72%, and 51% for rudbeckia at 200 ml (0.3 in), 400 ml (0.6 in), 800 ml (1.1 in) and 1200 ml (1.7 in), respectively. NH4-N and NO3-N and PO4-P concentrations in substrate solution decreased with increasing irrigation volume regardless of CRF. Substrate NH4-N concentration decreased throughout the season with most CRFs below 5 mg/liter by 90 DAI. CRFs mainly affected substrate NH4-N and NO3-N concentrations when irrigated with 200 ml (0.3 in) or 400 ml (0.6 in). Substrate NH4-N, NO3-N, and PO4-P solution concentrations were similar for all CRFs at irrigation volume of 1200 ml (1.7 in). Osmocote, Scotts, and Woodace maintained relatively constant substrate solution levels of PO4-P through 60 DAI. By 90 DAI, substrate PO4-P levels were similar regardless of irrigation volume or CRF. Substrate PO4-P concentrations were never in the recommended range of 5 to 10 mg/liter when irrigated with 800 ml (1.1 in) or 1200 ml (1.7 in) regardless of CRF. Solution pH remained in the recommended range of 5.0 to 6.0 for all irrigation volumes and CRFs throughout the entire study with the exception of Sustane.

Nitrogen gross balance in farms specialised in plant production in selected regions of Poland

2011 ◽  
Vol 66 (1) ◽  
Author(s):  
Jerzy Kupiec ◽  
Agnieszka Zdanowska ◽  
Janina Zbierska ◽  
Aleksandra Woźniak
Keyword(s):  
Plant Production

Profitability of dairy farming in relation to the type of feeding system

2011 ◽  
Vol 28 (1) ◽  
pp. 55-59 ◽  
Author(s):  
Jan Frelich ◽  
Martin Šlachta ◽  
František Střeleček ◽  
Jana Lososová
Keyword(s):  
Arable Land ◽  
Principal Component ◽  
Dairy Farming ◽  
Plant Production ◽  
Feeding Strategies ◽  
Feeding System ◽  
Financial Health ◽  
Profit Rate ◽  
Permanent Housing ◽  
Cow Performance

Profitability of dairy farming in relation to the type of feeding systemWe investigated the profitability of dairy farms in relation to the type of feeding system (seasonal pasture vs. permanent housing). An economic analysis was carried out of data on the structure and financial health of 50 farms in 2007 using questionnaires filled in by the farmers. The Principal Component Analysis (PCA) was applied to reveal causal relationships between a number of characteristics of the farms. The two axis of PCA explained 40.48% and 16.13% of the variability among the selected farm characteristics. Profitability related more to the number of subsidies, the area of arable land, the number of livestock and to the milk and plant production than to the area of meadows and pastures. Although a better cow performance was achieved on farms with confined herds, the profit per agricultural area and profit rate did not differ significantly between the two feeding strategies (P>0.05). The profit was 3,259 and 3,655 CZK/ha on average and the profit rate 7.9% and 5.6% on average on farms with pastured herds and on farms with confined herds, respectively. A lowering of input costs and a more effective utilisation of grasslands may further enhance profitability.

Current situation with the core collection of spring triticale of the National Center of Plant Genetic Resources of Ukraine

2018 ◽  
pp. 75-85
Author(s):  
S.V. Chernobai ◽  
V.K. Riabchun ◽  
T.B. Kapustina ◽  
V.S. Melnyk ◽  
O.E. Shchechenko
Keyword(s):  
Genetic Resources ◽  
Plant Genetic Resources ◽  
Plant Production ◽  
Educational Institutions ◽  
Breeding Lines ◽  
Growing Season Length ◽  
Long Term Storage ◽  
Foreign Countries ◽  
Spring Triticale

Goal. To build up a spring triticale genetic bank to provide breeding, scientific and educational institutions with initial material and to preserve the existing diversity. To update the database of accessions with a set of valuable economic and morphological features. Results and discussion. The methodology and results of the collection formation and evaluation of spring triticale accessions in the National Center for Plant Genetic Resources of Ukraine of Plant Production Institute nd. a V. Ya. Yuriev are presented. The formed collection includes 1,935 accessions from 27 countries: 42 varieties and 1,478 breeding lines from Ukraine, 92 varieties and 248 lines from foreign countries and also 75 genetic lines. The collection was formed by major valuable economic features (plant height, growing season length, spike threshing, yield, 1000-grain weight, disease resistance, technological properties, etc.). Accessions with the majority of morpho-biological and valuable economic features were selected. All the accessions in the collection are certificated. 1,762 accessions were packed for storage into the National Depository; 1,507 of them were packed for long-term storage. Conclusions. The gene pool of spring triticale from the collection of the Gene Bank of Plants of Ukraine is widely used for breeding. This allows conducting hybridization of genetically and ecologically remote forms with various expressions of features and obtaining whole new breeding material. Involvement of collection accessions in breeding allows generating new genetic sources of valuable economic features.

Phosphorus Fertilization in Ebb and Flow Production of Bedding Plants

HortScience ◽  
1998 ◽  
Vol 33 (3) ◽  
pp. 523b-523 ◽  
Author(s):  
Erin James ◽  
Marc van Iersel
Keyword(s):  
Dry Mass ◽  
Plant Production ◽  
Phosphorus Fertilization ◽  
Time Interval ◽  
Nutrient Runoff ◽  
Growing Period ◽  
Bedding Plants ◽  
Lack Of Information ◽  
Bedding Plant ◽  
Ebb And Flow

The quantity and quality of available water in the Southeastern United States continues to decline as demands on limited resources increase. Growers will soon be forced to comply with legal limitations on water consumption and limits on nutrient runoff from their operations. A lack of information on standard growing practices using alternative irrigation systems such as ebb and flow is hindering their acceptance and implementation. We are currently conducting a series of experiments to establish basic growing guidelines for the use of ebb and flow in the greenhouse in bedding plant production. In the third of these experiments, Petunia × hybrida Hort. Vilm.-Andr. `Blue Frost' and Begonia × hiemalis Fotsch. `Ambassador Scarlet' were grown for 5 weeks on ebb and flow tables with fertigation solutions (225 ppm N) containing three different levels of phosphorus (0, 50, and 100 ppm). Three soilless media were also used, which varied in their percentage content of vermiculite, perlite, pine bark and coconut coir. For both the begonias and petunias dry mass of the shoot was greatest in plants grown with higher levels of phosphorus. In comparison to plants grown with 0 ppm phosphorous, petunias and begonias grown with 50 or 100 ppm P were 44% and 25% greater in mass, respectively. However, begonias had 38% more flowers when fertigated with the higher levels of phosphorous while petunias flowered earlier with 0 ppm P fertigation solution. The electrical conductivity of the media did not change significantly over the course of the growing period, but the pH dropped by an average of 1 over the same time interval.

Impact of Fertigation versus Controlled-release Fertilizer Formulations on Nitrate Concentrations in Nursery Drainage Water

2011 ◽  
Vol 21 (2) ◽  
pp. 176-180 ◽  
Author(s):  
P. Chris Wilson ◽  
Joseph P. Albano
Keyword(s):  
Water Quality ◽  
Controlled Release ◽  
Drainage Water ◽  
Plant Production ◽  
Fertilization Program ◽  
Median Concentration ◽  
Nitrate N ◽  
Production Area ◽  
Foliage Plant ◽  
Production Areas

Nitrate-nitrogen (N) losses in surface drainage and runoff water from ornamental plant production areas can be considerable. In N-limited watersheds, discharge of N from production areas can have negative impacts on nontarget aquatic systems. This study monitored nitrate-N concentrations in production area drainage water originating from a foliage plant production area. Concentrations in drainage water were monitored during the transition from 100% reliance on fertigation using urea and nitrate-based soluble formulations (SF) to a nitrate-based controlled-release formulation (CRF). During the SF use period, nitrate-N concentrations ranged from 0.5 to 322.0 mg·L−1 with a median concentration of 31.2 mg·L−1. Conversely, nitrate-N concentrations during the controlled-release fertilization program ranged from 0 to 147.9 mg·L−1 with a median concentration of 0.9 mg·L−1. This project demonstrates that nitrate-N concentrations in drainage water during the CRF program were reduced by 94% to 97% at the 10th through 95th percentiles relative to the SF fertilization program. Nitrate-N concentrations in drainage water from foliage plant production areas can be reduced by using CRF fertilizer formulations relative to SF formulations/fertigation. Similar results should be expected for other similar containerized crops. Managers located within N-limited watersheds facing N water quality regulations should consider the use of CRF fertilizer formulations as a potential tool (in addition to appropriate application rates and irrigation management) for reducing production impacts on water quality.

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