Real-Time AFMs Combined with Inverted Optical Microscopes for Wet Cell/Tissue Imaging

2016 ◽  
pp. 197-210
Keyword(s):  
Real Time ◽  
Tissue Imaging ◽  
Cell Tissue

scholarly journals Real-time observation of tetrapyrrole binding to an engineered bacterial phytochrome

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Yusaku Hontani ◽  
Mikhail Baloban ◽  
Francisco Velazquez Escobar ◽  
Swetta A. Jansen ◽  
Daria M. Shcherbakova ◽  
...  
Keyword(s):  
Real Time ◽  
Rational Design ◽  
Near Infrared ◽  
Fluorescent Proteins ◽  
Covalent Bond ◽  
Binding Pocket ◽  
Tissue Imaging ◽  
Covalent Attachment ◽  
Time Resolved

AbstractNear-infrared fluorescent proteins (NIR FPs) engineered from bacterial phytochromes are widely used for structural and functional deep-tissue imaging in vivo. To fluoresce, NIR FPs covalently bind a chromophore, such as biliverdin IXa tetrapyrrole. The efficiency of biliverdin binding directly affects the fluorescence properties, rendering understanding of its molecular mechanism of major importance. miRFP proteins constitute a family of bright monomeric NIR FPs that comprise a Per-ARNT-Sim (PAS) and cGMP-specific phosphodiesterases - Adenylyl cyclases - FhlA (GAF) domain. Here, we structurally analyze biliverdin binding to miRFPs in real time using time-resolved stimulated Raman spectroscopy and quantum mechanics/molecular mechanics (QM/MM) calculations. Biliverdin undergoes isomerization, localization to its binding pocket, and pyrrolenine nitrogen protonation in <1 min, followed by hydrogen bond rearrangement in ~2 min. The covalent attachment to a cysteine in the GAF domain was detected in 4.3 min and 19 min in miRFP670 and its C20A mutant, respectively. In miRFP670, a second C–S covalent bond formation to a cysteine in the PAS domain occurred in 14 min, providing a rigid tetrapyrrole structure with high brightness. Our findings provide insights for the rational design of NIR FPs and a novel method to assess cofactor binding to light-sensitive proteins.

Telerobotic system concept for real-time soft-tissue imaging during radiotherapy beam delivery

2010 ◽  
Vol 37 (12) ◽  
pp. 6357-6367 ◽  
Author(s):  
Jeffrey Schlosser ◽  
Kenneth Salisbury ◽  
Dimitre Hristov
Keyword(s):  
Soft Tissue ◽  
Real Time ◽  
Tissue Imaging ◽  
System Concept ◽  
Beam Delivery

Applying RGB LED in full-field optical coherence tomography for real-time full-color tissue imaging

2014 ◽  
Vol 53 (22) ◽  
pp. E56 ◽  
Author(s):  
Bor-Wen Yang ◽  
Yu-Yen Wang ◽  
Yu-Min Lin ◽  
Yu-Shan Juan ◽  
Hung-Te Chen ◽  
...  
Keyword(s):  
Optical Coherence Tomography ◽  
Real Time ◽  
Tissue Imaging ◽  
Optical Coherence ◽  
Full Field ◽  
Full Color

MP-4.16: Real-Time Tissue Imaging of Prostate and Periprostatic Neural Tissue with Multiphoton Microscopy

Urology ◽  
2008 ◽  
Vol 72 (5) ◽  
pp. S89-S90
Author(s):  
R. Yadav ◽  
S. Mukherjee ◽  
F. Maxfield ◽  
J. Jhaveri ◽  
R. Leung ◽  
...  
Keyword(s):  
Real Time ◽  
Multiphoton Microscopy ◽  
Neural Tissue ◽  
Tissue Imaging

Real-time interlaced ultrasound and photoacoustic system forin vivoovarian tissue imaging

2013 ◽  
Author(s):  
Umar Alqasemi ◽  
Hai Li ◽  
Guangqian Yuan ◽  
Patrick Kumavor ◽  
Saeid Zanganeh ◽  
...  
Keyword(s):  
Real Time ◽  
Tissue Imaging
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