A Sensitive Cell Suspension Enzyme-Linked Immunosorbent Assay Measuring the Density of Cell Surface Ganglioside and Carbohydrate Antigens

1997 ◽  
pp. 161-190
Keyword(s):  
Cell Surface ◽  
Cell Suspension ◽  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Sensitive Cell ◽  
Carbohydrate Antigens

Quantitation of the density of cell surface carbohydrate antigens on cancer cells with a sensitive cell-suspension ELISA

1996 ◽  
Vol 197 (1-2) ◽  
pp. 51-67 ◽  
Author(s):  
Mepur H. Ravindranath ◽  
Philip M. Bauer ◽  
Cromwell Cornillez-Ty ◽  
Janet Garcia ◽  
Donald L. Morton
Keyword(s):  
Cell Surface ◽  
Cell Suspension ◽  
Cancer Cells ◽  
Sensitive Cell ◽  
Carbohydrate Antigens ◽  
Cell Surface Carbohydrate

scholarly journals Analysis ofLeishmaniamimetic neoglycoproteins for the cutaneous leishmaniasis diagnosis

Parasitology ◽  
2018 ◽  
Vol 145 (14) ◽  
pp. 1938-1948 ◽  
Author(s):  
Lígia Moraes Barizon de Souza ◽  
Vanete Thomaz Soccol ◽  
Ricardo Rasmussen Petterle ◽  
Michelle D. Bates ◽  
Paul A. Bates
Keyword(s):  
Cell Surface ◽  
Cutaneous Leishmaniasis ◽  
Sensitivity And Specificity ◽  
Immunosorbent Assay ◽  
Serum Levels ◽  
Enzyme Linked Immunosorbent Assay ◽  
Healthy Individuals ◽  
Cell Surfaces ◽  
Cell Markers ◽  
Antibody Titres

AbstractOligosaccharides are broadly present onLeishmaniacell surfaces. They can be useful for the leishmaniases diagnosis and also helpful in identifying new cell markers for the disease. The disaccharide Galα1-3Galβis the immunodominant saccharide inLeishmaniacell surface and is the unique non-reducing terminal glycosphingolipids structure recognized by anti-α-Gal. This study describes an enzyme-linked immunosorbent assay (ELISA) used to measure serum levels of anti-α-galactosyl (α-Gal) antibodies in patients with cutaneous leishmaniasis (CL). Optimal ELISA conditions were established and two neoglycoproteins (NGP) containing the Galα1-3Gal terminal fraction (Galα1-3Galβ1-4GlcNAc-HAS and Galα1-3Gal-HAS) and one Galα1-3Gal NGP analogue (Galα1-3Galβ1-3GlcNAc-HAS) were used as antigens. Means of anti-α-Gal antibody titres of CL patients were significantly higher (P< 0.05) than the healthy individuals for all NGPs tested. Sensitivity and specificity of all NGPs ranged from 62.2 to 78.4% and 58.3 to 96.7%, respectively. In conclusion, the NGPs can be used for CL diagnosis.

scholarly journals Limitations of Pneumovax® as a detection antigen in the measurement of serotype-specific antibodies by enzyme-linked immunosorbent assay

2002 ◽  
Vol 39 (4) ◽  
pp. 398-403 ◽  
Author(s):  
ZM Huo ◽  
J Miles ◽  
PG Riches ◽  
T Harris
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carbohydrate Antigens ◽  
Specific Antibodies ◽  
Elisa System ◽  
Background Measurement ◽  
Polysaccharide Antigens ◽  
The Individual

Background: Measurement of antibody responses to polysaccharide antigens is regarded as an important assessment of an individual's ability to respond to carbohydrate antigens. The currently used assays for the measurement of pneumococcal-specific antibody use the multi-serotype vaccine Pneumovax® as the detection antigen. Methods: An equal potency enzyme-linked immunosorbent assay (ELISA) system was used to compare the measurement of serotype-specific antibody with the multi-serotype assay. Results: Our results show that the concentration of specific antibody to Pneumovax is not related to the concentration of antibody to the individual serotypes. Neither is any correlation found between the antibody concentrations to any of the three single serotypes investigated, to the mixture of the three serotypes or to Pneumovax. Conclusion: We conclude that the measurement of the concentration of the specific antibody to the mixed serotypes present in Pneumovax has serious limitations when used to evaluate the protection acquired from Pneumovax immunization against any specific serotype.

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