Detection ofLegionella antibodies by enzyme-linked immunosorbent assay (ELISA) using whole cell and carbohydrate antigens

1982 ◽  
Vol 8 (4) ◽  
pp. 287-298 ◽  
Author(s):  
Helen N. Westfall ◽  
William F. Myers ◽  
Emilio Weiss
Keyword(s):  
Immunosorbent Assay ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carbohydrate Antigens ◽  
Whole Cell

scholarly journals Vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in its fry against Aeromonas hydrophila

2017 ◽  
Vol 16 (2) ◽  
pp. 268 ◽  
Author(s):  
Sukenda Sukenda ◽  
Odang Carman ◽  
Rahman Rahman ◽  
Dendi Hidayatullah ◽  
Nurfitriani Siti Yumaidawati
Keyword(s):  
Disease Resistance ◽  
Immunosorbent Assay ◽  
Antibody Level ◽  
Aeromonas Hydrophila ◽  
Nile Tilapia ◽  
Cell Vaccine ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whole Cell ◽  
Whole Cell Vaccine ◽  
Phosphate Buffered Saline

<p class="NoParagraphStyle"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">The aim of this study was to analyze the effectivity of vaccination in Nile tilapia broodstock with whole cell vaccine and disease resistance in fry tilapia against <em>Aeromonas hydrophila</em>. Tilapia Nirwana strain that used for this had average body weight of 185±13.23 g and were maintained in ponds sizing of (2.5×2.5×1 m<sup>3</sup>). Vaccinations that has been done through intraperitoneal injection using dose of 0.1 mL/fish, meanwhile the fish for control was injected by phosphate buffered saline (PBS). This study used complete randomized design with two treatments and three replications. Antibody level was measured by using indirect enzyme-linked immunosorbent assay (ELISA) method in the broodstock, egg, and fry.  Challenge test in fry tilapia performed at the age of 5, 10, and 15 days. The results showed that vaccination in tilapia broodstock delivered a significant antibody level in broodstock, eggs, and fry (P&lt;0.05) compared to the control. Relative percent survival of offspring at 5, 10, and 15 days were 78.26%, 70.59%, and 65.52%, respectively.  As a conclusion, vaccination in tilapia broodstock was effective to improve specific and non-specific immunity, and protect fry tilapia from <em>A. hydrophila</em> infection through maternal immunity.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Keywords: vaccination, antibody, maternal immunity, tilapia, <em>Aeromonas hydrophila</em></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle"><strong>ABSTRAK</strong><strong></strong></p><p class="NoParagraphStyle"><strong> </strong></p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk menganalisis efikasi vaksinasi pada induk nila dengan vaksin sel utuh dan ketahanan benih yang dihasilkan terhadap <em>Aeromonas hydrophila</em>. Ikan nila stain Nirwana yang digunakan dalam penelitian memiliki bobot rata-rata 185±13,23 g dan ikan dipelihara dalam kolam (2,5×2,5×1 m<sup>3</sup>). vaksinasi dilakukan melalui penyuntikan intraperitoneal dengan dosis 0,1 mL/ikan, sementara itu ikan kontrol disuntik dengan <em>phosphate buffered saline</em> (PBS). Penelitian ini menggunakan rancangan acak lengkap dengan dua perlakuan dan tiga ulangan. Tingkat antibodi diukur dengan menggunakan metode<em> indirect enzyme-linked immunosorbent assay</em> (ELISA) pada induk, telur dan benih. Uji tantang pada benih dilakukan pada umur 5, 10, dan 15 hari. Hasil penelitian menunjukan bahwa vaksinasi pada induk nila secara signifikan dapat meningkatkan level antibodi pada induk, telur, dan benih (P&lt;0,05) dibandingkan dengan kontrol. Kelangsungan hidup relatif pada benih berumur 5, 10, dan 15 hari masing-masing adalah 78,26%; 70,59%; dan 65,52%. Sebagai kesimpulan vaksinasi pada induk nila efektif dalam memperbaiki imunitas spesifik dan non spesifik serta melindungi benih dari infeksi <em>A. hydrophila</em> melalui imunitas maternal.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: vaksinasi, antibodi, imunitas maternal, ikan nila, <em>Aeromonas hydrophila</em></p>

scholarly journals Limitations of Pneumovax® as a detection antigen in the measurement of serotype-specific antibodies by enzyme-linked immunosorbent assay

2002 ◽  
Vol 39 (4) ◽  
pp. 398-403 ◽  
Author(s):  
ZM Huo ◽  
J Miles ◽  
PG Riches ◽  
T Harris
Keyword(s):  
Immunosorbent Assay ◽  
Specific Antibody ◽  
Antibody Responses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Carbohydrate Antigens ◽  
Specific Antibodies ◽  
Elisa System ◽  
Background Measurement ◽  
Polysaccharide Antigens ◽  
The Individual

Background: Measurement of antibody responses to polysaccharide antigens is regarded as an important assessment of an individual's ability to respond to carbohydrate antigens. The currently used assays for the measurement of pneumococcal-specific antibody use the multi-serotype vaccine Pneumovax® as the detection antigen. Methods: An equal potency enzyme-linked immunosorbent assay (ELISA) system was used to compare the measurement of serotype-specific antibody with the multi-serotype assay. Results: Our results show that the concentration of specific antibody to Pneumovax is not related to the concentration of antibody to the individual serotypes. Neither is any correlation found between the antibody concentrations to any of the three single serotypes investigated, to the mixture of the three serotypes or to Pneumovax. Conclusion: We conclude that the measurement of the concentration of the specific antibody to the mixed serotypes present in Pneumovax has serious limitations when used to evaluate the protection acquired from Pneumovax immunization against any specific serotype.

scholarly journals MIC1-MAG1-SAG1 Chimeric Protein, a Most Effective Antigen for Detection of Human Toxoplasmosis

2012 ◽  
Vol 19 (12) ◽  
pp. 1977-1979 ◽  
Author(s):  
Lucyna Holec-Gąsior ◽  
Bartłomiej Ferra ◽  
Dorota Drapała
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Protein A ◽  
Chimeric Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whole Cell ◽  
Content Type

ABSTRACTThis study describes aToxoplasma gondiiIgG enzyme-linked immunosorbent assay based on a new chimeric antigen containing three immunodominant regions from the MIC1, MAG1, and SAG1 proteins of the parasite and shows that this test is useful for diagnostic purposes and may replace the lysed and whole-cell antigens.

scholarly journals Direct Human Papillomavirus E6 Whole-Cell Enzyme-Linked Immunosorbent Assay for Objective Measurement of E6 Oncoproteins in Cytology Samples

2012 ◽  
Vol 19 (9) ◽  
pp. 1474-1479 ◽  
Author(s):  
Yi-Shan Yang ◽  
Karen Smith-McCune ◽  
Teresa M. Darragh ◽  
Yvonne Lai ◽  
Ju-Hwa Lin ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
High Risk ◽  
Immunosorbent Assay ◽  
Objective Measurement ◽  
Enzyme Linked Immunosorbent Assay ◽  
Whole Cell ◽  
Hpv Dna ◽  
Hpv E6 ◽  
Cell Enzyme ◽  
Grade 3

ABSTRACTA novel, whole-cell enzyme-linked immunosorbent assay (ELISA) based on a non-type-specific anti-human papillomavirus (HPV) E6 antibody was tested on 182 residual cytological specimens. For samples with a designation of more severe thancervicalintraepithelialneoplasia grade 3 (CIN3+), 83% tested positive for E6; in a subset with paired testing for E6 ELISA and HPV DNA, 72% tested E6 positive and 92% tested high-risk (HR)-HPV DNA positive (P= 0.2). Among the women with a less than CIN3 diagnosis, 31% and 47% tested positive for E6 and HR-HPV DNA, respectively (P= 0.0006).

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