Immunohistochemical Methods

2013 ◽  
pp. 317-356
Keyword(s):  
Immunohistochemical Methods

Cellular Localisation in Placenta of Placental Type Plasminogen Activator Inhibitor

1986 ◽  
Vol 56 (01) ◽  
pp. 063-065 ◽  
Author(s):  
B Åstedt ◽  
I Hägerstrand ◽  
I Lecander
Keyword(s):  
Monoclonal Antibodies ◽  
Plasminogen Activator ◽  
Plasminogen Activator Inhibitor ◽  
Immunoreactive Material ◽  
Type Plasminogen Activator ◽  
Cellular Localisation ◽  
Immunohistochemical Methods ◽  
Activator Inhibitor ◽  
In Pregnancy

SummaryA specific plasminogen activator inhibitor is known to occur in placenta and in pregnancy plasma. Immunohistochemical methods with polyclonal and monoclonal antibodies against the inhibitor were used for its localisation in term placentas. Immunoreactive material was found in the trophoblastic epithelium. It was absent in the stroma of the chorion villi.

scholarly journals Ichthyoses—A Clinical and Pathological Spectrum from Heterogeneous Cornification Disorders to Inflammation

2021 ◽  
Vol 8 (2) ◽  
pp. 107-123
Author(s):  
Dieter Metze ◽  
Heiko Traupe ◽  
Kira Süßmuth
Keyword(s):  
Stratum Granulosum ◽  
Internal Organs ◽  
Histopathologic Diagnosis ◽  
Epidermolytic Ichthyosis ◽  
Life Threatening ◽  
Keratinization Disorders ◽  
Gene Defects ◽  
Immunohistochemical Methods

Ichthyoses are inborn keratinization disorders affecting the skin only (non-syndromic) or are associated with diseases of internal organs (syndromic). In newborns, they can be life-threatening. The identification of the gene defects resulted in reclassification and a better understanding of the pathophysiology. Histopathologic patterns include orthohyperkeratosis with a reduced or well-developed stratum granulosum, hyperkeratosis with ortho- and parakeratosis with preserved or prominent stratum granulosum, and epidermolytic ichthyosis. Another pattern features “perinuclear vacuoles and binucleated keratinocytes”, which is associated with keratin mutations. Some ichthyoses are histologically defined by psoriasis-like features, and distinct subtypes show follicular hyperkeratosis. In addition to histological and immunohistochemical methods, these patterns allow a better histopathologic diagnosis.

scholarly journals Extracellular Protein Aggregates Colocalization and Neuronal Dystrophy in Comorbid Alzheimer’s and Creutzfeldt–Jakob Disease: A Micromorphological Pilot Study on 20 Brains

2021 ◽  
Vol 22 (4) ◽  
pp. 2099
Author(s):  
Nikol Jankovska ◽  
Tomas Olejar ◽  
Radoslav Matej
Keyword(s):  
Prion Protein ◽  
Fluorescent Microscopy ◽  
Amyloid Β ◽  
Amyloid Β Protein ◽  
Β Protein ◽  
Key Characteristics ◽  
Jakob Disease ◽  
Immunohistochemical Methods ◽  
Confocal Fluorescent Microscopy ◽  
Codon 129

Alzheimer’s disease (AD) and sporadic Creutzfeldt–Jakob disease (sCJD) are both characterized by extracellular pathologically conformed aggregates of amyloid proteins—amyloid β-protein (Aβ) and prion protein (PrPSc), respectively. To investigate the potential morphological colocalization of Aβ and PrPSc aggregates, we examined the hippocampal regions (archicortex and neocortex) of 20 subjects with confirmed comorbid AD and sCJD using neurohistopathological analyses, immunohistochemical methods, and confocal fluorescent microscopy. Our data showed that extracellular Aβ and PrPSc aggregates tended to be, in most cases, located separately, and “compound” plaques were relatively rare. We observed PrPSc plaque-like structures in the periphery of the non-compact parts of Aβ plaques, as well as in tau protein-positive dystrophic structures. The AD ABC score according to the NIA-Alzheimer’s association guidelines, and prion protein subtype with codon 129 methionine–valine (M/V) polymorphisms in sCJD, while representing key characteristics of these diseases, did not correlate with the morphology of the Aβ/PrPSc co-aggregates. However, our data showed that PrPSc aggregation could dominate during co-aggregation with non-compact Aβ in the periphery of Aβ plaques.

scholarly journals Development of Immunohistochemical Methods for Casein Detection in Meat Products

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 28
Author(s):  
Ludmila Kalčáková ◽  
Matej Pospiech ◽  
Bohuslava Tremlová ◽  
Zdeňka Javůrková ◽  
Irina Chernukha
Keyword(s):  
Milk Protein ◽  
Production Efficiency ◽  
Raw Materials ◽  
Meat Products ◽  
Milk Proteins ◽  
Increase Production Efficiency ◽  
Allergenic Potential ◽  
Significant Difference ◽  
Dairy Ingredients ◽  
Immunohistochemical Methods

To increase production efficiency of meat products, milk protein additives are often used. Despite a number of advantages, use of dairy ingredients involves a certain risk, namely the allergenic potential of milk proteins. A number of methods have been developed to detect milk-origin raw materials in foodstuffs, including immunological reference methods. This study presents newly developed immunohistochemical (IHC) methods for casein detection in meat products. Casein was successfully detected directly in meat products where sensitivity was determined at 1.21 and specificity at 0.28. The results obtained from the IHC were compared with the Enzyme-Linked Immuno Sorbent Assay (ELISA) and there was no statistically significant difference between the IHC and ELISA methods (p > 0.05). The correspondence between the methods was 72% in total. The highest correspondence was reached in frankfurters (90%), the lowest in canned pâté (44%).

scholarly journals Localization of Receptors for Sex Steroids and Pituitary Hormones in the Female Genital Duct throughout the Reproductive Cycle of a Viviparous Gymnophiona Amphibian, Typhlonectes compressicauda

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 2
Author(s):  
Claire Brun ◽  
Jean-Marie Exbrayat ◽  
Michel Raquet
Keyword(s):  
Reproductive Cycle ◽  
Sex Steroids ◽  
Morphological Changes ◽  
Progesterone Receptors ◽  
Pituitary Hormones ◽  
Prolactin Receptors ◽  
Functional Regulation ◽  
Specific Receptors ◽  
Immunohistochemical Methods ◽  
Female Genital

Reproduction in vertebrates is controlled by the hypothalamo-pituitary-gonadal axis, and both the sex steroid and pituitary hormones play a pivotal role in the regulation of the physiology of the oviduct and events occurring within the oviduct. Their hormonal actions are mediated through interaction with specific receptors. Our aim was to locate α and β estrogen receptors, progesterone receptors, gonadotropin and prolactin receptors in the tissues of the oviduct of Typhlonectes compressicauda (Amphibia, Gymnophiona), in order to study the correlation between the morphological changes of the genital tract and the ovarian cycle. Immunohistochemical methods were used. We observed that sex steroids and pituitary hormones were involved in the morpho-functional regulation of oviduct, and that their cellular detection was dependent on the period of the reproductive cycle.

An Assessment of Human Epidermal Repair in Elderly Normal Subjects Using Immunohistochemical Methods

1988 ◽  
Vol 90 (6) ◽  
pp. 845-850 ◽  
Author(s):  
John E. Olerud ◽  
Allen M. Gown ◽  
Jackie. Bickenbach ◽  
Beverly. Dale ◽  
George F. Odland
Keyword(s):  
Normal Subjects ◽  
Immunohistochemical Methods

MINERALIZATION OF TEETH ENAMEL AFTER ERUPTION

2021 ◽  
Vol 74 (6) ◽  
pp. 1297-1301
Author(s):  
Oleksij P. Kostyrenko ◽  
Nataliia I. Vynnyk ◽  
Mykhailo M. Koptev ◽  
Petro A. Hasiuk ◽  
Maksym I. Skrypnyk ◽  
...  
Keyword(s):  
Light And Electron Microscopy ◽  
Permanent Tooth ◽  
Postnatal Period ◽  
Permanent Teeth ◽  
Tooth Crown ◽  
Electron Microscopic ◽  
Cervical Portion ◽  
Immunohistochemical Methods ◽  
Protein Deposit ◽  
Different Parts

The aim: The paper was aimed at the study of the processes of mineralization of the enamel of the permanent tooth after its eruption. Materials and methods: To study the structure of the enamel of permanent teeth has been carried out using light and electron microscopy. The study of the process of the development of the primordia of the permanent teeth involved 10 culled puppies of 30-40 days of age. Microscopic, electron microscopic, immunohistochemical methods of research have been used to study the processes of histogenesis. Results: The studies show that in the postnatal period, the formation of the crown, externally covered with cuticular epithelium, marks the formation of the primordium of the permanent tooth at the follicle stage. After eruption of a tooth, different parts of its crown have three individual structural and functional barriers to enamel biomineralization. The first one is provided by the cuticular epithelium of the pitted areas of the crown, which ensures filtering of the salivary fluid from the protein deposit in the form of a pellicle. The second barrier is defined on the lateral and cuspidate surfaces of the enamel, where the cuticle is erased or poorly expressed. The third structural and functional barrier of enamel biomineralization is located in the cervical portion of teeth of different classes. Conclusions: Different areas of the enamel in the tooth crown have specific filtration barriers, which can be distinguished as follows: pit-and-fissure-and-groove, cuspidateand-approximal, and cervical barriers. The cuticle is poorly expressed or totally absent on the cusps of the tooth crowns in contrast to pitted areas.

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