scholarly journals Localization of Receptors for Sex Steroids and Pituitary Hormones in the Female Genital Duct throughout the Reproductive Cycle of a Viviparous Gymnophiona Amphibian, Typhlonectes compressicauda

Animals ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 2
Author(s):  
Claire Brun ◽  
Jean-Marie Exbrayat ◽  
Michel Raquet
Keyword(s):  
Reproductive Cycle ◽  
Sex Steroids ◽  
Morphological Changes ◽  
Progesterone Receptors ◽  
Pituitary Hormones ◽  
Prolactin Receptors ◽  
Functional Regulation ◽  
Specific Receptors ◽  
Immunohistochemical Methods ◽  
Female Genital

Reproduction in vertebrates is controlled by the hypothalamo-pituitary-gonadal axis, and both the sex steroid and pituitary hormones play a pivotal role in the regulation of the physiology of the oviduct and events occurring within the oviduct. Their hormonal actions are mediated through interaction with specific receptors. Our aim was to locate α and β estrogen receptors, progesterone receptors, gonadotropin and prolactin receptors in the tissues of the oviduct of Typhlonectes compressicauda (Amphibia, Gymnophiona), in order to study the correlation between the morphological changes of the genital tract and the ovarian cycle. Immunohistochemical methods were used. We observed that sex steroids and pituitary hormones were involved in the morpho-functional regulation of oviduct, and that their cellular detection was dependent on the period of the reproductive cycle.

Progestin receptors in prolactin and growth hormone producing tumours in rats

1982 ◽  
Vol 100 (1) ◽  
pp. 25-30 ◽  
Author(s):  
Oddvar Naess ◽  
Egil Haug ◽  
Arne Attramadal ◽  
Kaare M. Gautvik
Keyword(s):  
Growth Hormone ◽  
Glucocorticoid Receptors ◽  
Progesterone Receptors ◽  
Pituitary Tumour ◽  
Tumour Cells ◽  
Female Rats ◽  
Progestin Receptors ◽  
High Affinity ◽  
Protein Nature ◽  
Specific Receptors

Abstract. Progesterone and corticosterone have a similar effect on the production of growth hormone (GH) and prolactin (Prl) by pituitary tumour cells (GH3 cells) in culture. Previously we have shown that progesterone has a high affinity for the glucocorticoid receptors in these cells. Progesterone may therefore exert its effects through binding to the glucocorticoid receptor. The aim of the present study was to investigate if the GH3 tumour cells and an oestrogen induced pituitary tumour, which also produce GH and Prl, possess specific receptors for progesterone. Both the GH3 tumours and the oestrogen induced pituitary tumour were in fact found to possess cytoplasmatic receptor molecules for progesterone by using the potent progestin R5020 as a marker. Isoelectric focusing revealed one binding component (pH 5.9), which was of protein nature. The binding was of high affinity (Kd 2 × 10−9 mol/l). In the oestrogen induced tumour, the maximal binding was 70 fmol/mg cytosol protein. In female rats with GH3 tumours the binding was 55 fmol/mg cytosol protein. Priming of the animals with 1 mg oestradiol-valerate increased the binding to 116 fmol/mg cytosol protein, whereas very little binding was found in GH3 tumours from rats castrated 7 days before sacrifice. The receptors in the oestrogen induced pituitary tumour and the GH3 tumours exhibited high affinity for R5020 and progesterone, whereas corticosterone had no significant affinity for the receptors. Using exchange assay, it was demonstrated that the cytoplasmic progestin receptors could be translocated to the nucleus after administration of progesterone to the animals. Thus, the presence of specific progesterone receptors, different from the glucocorticoid receptors, strongly indicates that the effects of progesterone on GH and Prl production are mediated through the progesterone receptors.

The reproductive cycle of the endangered cyprinidAlburnus albidus: Morphological changes of the gonads and plasma sex steroid fluctuations

1998 ◽  
Vol 65 (sup1) ◽  
pp. 223-226 ◽  
Author(s):  
Giulia Guerriero ◽  
Marina Paolucci ◽  
Pier Giorgio Bianco ◽  
Virgilio Botte ◽  
Gaetano Ciarcia
Keyword(s):  
Reproductive Cycle ◽  
Morphological Changes ◽  
Sex Steroid

scholarly journals Reproductive cycle of the Neotropical cichlid yellow peacock bass Cichla kelberi: A novel pattern of testicular development

2013 ◽  
Vol 11 (3) ◽  
pp. 587-596 ◽  
Author(s):  
Diógenes Henrique de Siqueira-Silva ◽  
Carlos Alberto Vicentini ◽  
Alexandre Ninhaus-Silveira ◽  
Rosicleire Veríssimo-Silveira
Keyword(s):  
Reproductive Cycle ◽  
Germ Cells ◽  
Light Microscope ◽  
Morphological Changes ◽  
Germinal Epithelium ◽  
Testicular Development ◽  
Preparatory Phase ◽  
Testicular Morphology ◽  
Peacock Bass ◽  
Spermatogonial Proliferation

The present study describes the testicular maturation phases (associating the germ cells development and the morphological changes suffered by the germinal epithelium along the whole year), and the testicular morphology in the yellow peacock bass Cichla kelberi, relating it to other species. For this purpose, 78 specimens were studied according conventional techniques of light microscope. The testes in C. kelberi were classified as unrestricted spermatogonial lobular, an apomorphic characteristic in the recent groups of Teleost. Furthermore, were defined five testicular maturation phases: Preparatory phase; Early Germinal Epithelium Development; Mid Germinal Epithelium Development; Late Germinal Epithelium Development and; Regression. Similar classifications were described to other species indicating that the testicular classifications based on this propose, can be applied to lots of fishes. However, besides it similarity, the testicular reproductive cycle of C. kelberifollows a different pattern in the Regression phase, on which the gonadal restructuration and the spermatogonial proliferation gathers at the same time. So, the testes in C. kelberi never return to the Preparatory phase to start a new reproductive cycle, being this one present only at the first reproductive cycle in this species. This fact also explains the absence of individuals totally spent after their first reproductive cycle.

Testicular morphological changes during the seasonal reproductive cycle in the bullfrogRana catesbeiana

2004 ◽  
Vol 301A (3) ◽  
pp. 249-260 ◽  
Author(s):  
Estela Sasso-Cerri ◽  
Flávio P. De Faria ◽  
Edna Freymüller ◽  
Sandra M. Miraglia
Keyword(s):  
Reproductive Cycle ◽  
Morphological Changes

Steroid binding sites in liver membranes: Interplay between glucocorticoids, sex steroids, and pituitary hormones

2008 ◽  
Vol 109 (3-5) ◽  
pp. 336-343 ◽  
Author(s):  
L. Fernández-Pérez ◽  
A. Flores-Morales ◽  
R. Chirino-Godoy ◽  
J.C. Díaz-Chico ◽  
B.N. Díaz-Chico
Keyword(s):  
Sex Steroids ◽  
Binding Sites ◽  
Pituitary Hormones ◽  
Liver Membranes ◽  
Steroid Binding

scholarly journals Morphological analysis of interstitial Cajal cells and mast cells in experimental hyperactivity bladder and stress incontinence under influence of pharmacocorrection

2018 ◽  
Vol 24 (2) ◽  
pp. 5-13
Author(s):  
O.I. Iatsyna ◽  
S.V. Vernygorodskyi ◽  
F.I. Kostyev
Keyword(s):  
Urinary Incontinence ◽  
Stress Urinary Incontinence ◽  
Functional Activity ◽  
Morphological Changes ◽  
Bladder Wall ◽  
Experimental Models ◽  
Quantitative Composition ◽  
High Concentration ◽  
Cajal Cells ◽  
Immunohistochemical Methods

The existing data indicate the multifactorial mechanisms of development of the overactive bladder (OAB) symptom, but the issue of OAB pathogenesis remains unclear. In more recent times, the neurogenic theory of OAB genesis has being accompanied by the increasing attention to the study of morphological changes that occur in the smooth myocytes of the detrusor and their interaction with the extracellular matrix. Therefore, the objective of our study became the evaluation of distribution of interstitial Cajal cells (ICC) and basophilic granulocytes (BG) in the structural elements of the bladder wall under stress urinary incontinence and its overactivity before and after treatment with Mirabegron, Spasmex, Quercetin and combination thereof with testosterone and estradiol, using histochemical and immunohistochemical methods. The experimental models of OAB and stress urinary incontinence (SUI) presented the increase in the amount and functional activity of BG revealed by histological and immunohistochemical methods, as well as ICC at all terms of OAB monitoring, while the SUI presented with high concentration and functional activity of BG only after 14 days of the experiment. After 28 days, we observed a sharp decrease of the parameters, indicating decompensation and depletion of the functional activity. The number of ICC decreased under SUI after both 14 days and 28 days of the experiment. The group of experimental animals receiving Spasmex and its combination with hormones, presented no significant effect on the quantitative and qualitative composition of BG and ICC at OAB and SUI on Day 14 of the experiment, but the combination with testosterone demonstrated statistically reliable (p<0.001) reduction of BG and ICC expression in the muscle layer of the bladder after 14 days, and unreliable after 28 days of the experiment (p>0.05). The administration of Mirabegron alone and especially its combination with testosterone and estradiol presented positive trends in histochemical and immunohistochemical expression of BG and ICC. The experiment proved high efficacy of Quercetin in combination with testosterone and estradiol under OAB and SUI, confirmed by stabilization of the functional activity of BG and ICC quantitative composition.

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