Introductory Text and Sources of Stem Cells for Skeletal Tissue

2013 ◽  
pp. 1-1
Keyword(s):  
Stem Cells ◽  
Skeletal Tissue

Stem Cells and Their Use in Skeletal Tissue Repair

2010 ◽  
pp. 103-124
Author(s):  
Laura Baumgartner ◽  
Vuk Savkovic ◽  
Susanne Trettner ◽  
Colette Martin ◽  
Nicole I. zur Nieden
Keyword(s):  
Stem Cells ◽  
Tissue Repair ◽  
Skeletal Tissue

scholarly journals Stem Cells in Skeletal Tissue Engineering: Technologies and Models

2016 ◽  
Vol 11 (6) ◽  
pp. 453-474 ◽  
Author(s):  
Mark T. Langhans ◽  
Shuting Yu ◽  
Rocky S. Tuan
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Skeletal Tissue

scholarly journals Pluripotent stem cells for skeletal tissue engineering

2021 ◽  
pp. 1-20
Author(s):  
Miguel J. S. Ferreira ◽  
Fabrizio E. Mancini ◽  
Paul A. Humphreys ◽  
Leona Ogene ◽  
Michael Buckley ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Pluripotent Stem Cells ◽  
Skeletal Tissue

scholarly journals Extracellular matrix modulates the biological effects of melatonin in mesenchymal stem cells

2014 ◽  
Vol 223 (2) ◽  
pp. 167-180 ◽  
Author(s):  
Fan He ◽  
Xiaozhen Liu ◽  
Ke Xiong ◽  
Sijin Chen ◽  
Long Zhou ◽  
...  
Keyword(s):  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Biological Effects ◽  
Matrix Composition ◽  
Calcium Deposition ◽  
Specific Gene ◽  
Skeletal Tissue ◽  
Underlying Mechanisms

Both self-renewal and lineage-specific differentiation of mesenchymal stem cells (MSCs) are triggered by theirin vivomicroenvironment including the extracellular matrix (ECM) and secreted hormones. The ECM may modulate the physiological functions of hormones by providing binding sites and by regulating downstream signaling pathways. Thus, the purpose of this study was to evaluate the degree of adsorption of melatonin to a natural cell-deposited ECM and the effects of this interaction on the biological functions of melatonin in human bone marrow-derived MSCs (BM-MSCs). The fibrillar microstructure, matrix composition, and melatonin-binding affinity of decellularized ECM were characterized. The cell-deposited ECM improved melatonin-mediated cell proliferation by 31.4%, attenuated accumulation of intracellular reactive oxygen species accumulation, and increased superoxide dismutase (SOD) mRNA and protein expression. Interaction with ECM significantly enhanced the osteogenic effects of melatonin on BM-MSCs by increasing calcium deposition by 30.5%, up-regulating osteoblast-specific gene expression and down-regulating matrix metalloproteinase (MMP) expression. The underlying mechanisms of these changes in expression may involve intracellular antioxidant enzymes, because osteoblast-specific genes were down-regulated, whereas MMP expression was up-regulated, in the presence of SOD-specific inhibitors. Collectively, our findings indicate the importance of native ECM in modulating the osteoinductive and antioxidant effects of melatonin and provide a novel platform for studying the biological actions of growth factors or hormones in a physiologically relevant microenvironment. Moreover, a better understanding of the enhancement of MSC growth and osteogenic differentiation resulting from the combination of ECM and melatonin could improve the design of graft substitutes for skeletal tissue engineering.

scholarly journals Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration

2016 ◽  
Vol 13 (118) ◽  
pp. 20160182 ◽  
Author(s):  
Catarina Costa Moura ◽  
Rahul S. Tare ◽  
Richard O. C. Oreffo ◽  
Sumeet Mahajan
Keyword(s):  
Stem Cells ◽  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Therapeutic Potential ◽  
Natural State ◽  
Disease Treatment ◽  
Skeletal Tissue ◽  
Skeletal Disease ◽  
Anti Stokes ◽  
Skeletal Regeneration

The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.

scholarly journals Skeletal tissue engineering using embryonic stem cells

2010 ◽  
Vol 4 (3) ◽  
pp. 165-180 ◽  
Author(s):  
Jojanneke M. Jukes ◽  
Clemens A. van Blitterswijk ◽  
Jan de Boer
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Skeletal Tissue
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