scholarly journals Raman spectroscopy and coherent anti-Stokes Raman scattering imaging: prospective tools for monitoring skeletal cells and skeletal regeneration

2016 ◽  
Vol 13 (118) ◽  
pp. 20160182 ◽  
Author(s):  
Catarina Costa Moura ◽  
Rahul S. Tare ◽  
Richard O. C. Oreffo ◽  
Sumeet Mahajan
Keyword(s):  
Stem Cells ◽  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Therapeutic Potential ◽  
Natural State ◽  
Disease Treatment ◽  
Skeletal Tissue ◽  
Skeletal Disease ◽  
Anti Stokes ◽  
Skeletal Regeneration

The use of skeletal stem cells (SSCs) for cell-based therapies is currently one of the most promising areas for skeletal disease treatment and skeletal tissue repair. The ability for controlled modification of SSCs could provide significant therapeutic potential in regenerative medicine, with the prospect to permanently repopulate a host with stem cells and their progeny. Currently, SSC differentiation into the stromal lineages of bone, fat and cartilage is assessed using different approaches that typically require cell fixation or lysis, which are invasive or even destructive. Raman spectroscopy and coherent anti-Stokes Raman scattering (CARS) microscopy present an exciting alternative for studying biological systems in their natural state, without any perturbation. Here we review the applications of Raman spectroscopy and CARS imaging in stem-cell research, and discuss the potential of these two techniques for evaluating SSCs, skeletal tissues and skeletal regeneration as an exemplar.

Investigation of Microalgal Carotenoid Content Using Coherent Anti-Stokes Raman Scattering (CARS) Microscopy and Spontaneous Raman Spectroscopy

ChemPhysChem ◽  
2018 ◽  
Vol 19 (9) ◽  
pp. 1048-1055 ◽  
Author(s):  
Fisseha Bekele Legesse ◽  
Jan Rüger ◽  
Tobias Meyer ◽  
Christoph Krafft ◽  
Michael Schmitt ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Carotenoid Content ◽  
Cars Microscopy ◽  
Anti Stokes

scholarly journals Coherent anti-Stokes Raman scattering and spontaneous Raman spectroscopy and microscopy of microalgae with nitrogen depletion

2012 ◽  
Vol 3 (11) ◽  
pp. 2896 ◽  
Author(s):  
X. N. He ◽  
J. Allen ◽  
P. N. Black ◽  
T. Baldacchini ◽  
X. Huang ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Nitrogen Depletion ◽  
Anti Stokes

Rapid Raman spectroscopy of the excited electronic state of chrysene by coherent anti-stokes Raman scattering

1979 ◽  
Vol 61 (1) ◽  
pp. 105-108 ◽  
Author(s):  
W. Werncke ◽  
H.-J. Weigmann ◽  
J. Pätzold ◽  
A. Lau ◽  
K. Lenz ◽  
...  
Keyword(s):  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Electronic State ◽  
Excited Electronic State ◽  
Anti Stokes

scholarly journals Measurement of Silicon Nanoparticles Temperature by Raman Spectroscopy

2021 ◽  
Vol 7 (1) ◽  
pp. 010303
Author(s):  
Alida Alykova ◽  
Maria Grigoryeva ◽  
Irina Zavestovskaya ◽  
Victor Timoshenko
Keyword(s):  
Raman Spectroscopy ◽  
Laser Radiation ◽  
Cancer Therapy ◽  
Raman Scattering ◽  
Temperature Dependence ◽  
Radiation Intensity ◽  
Silicon Nanoparticles ◽  
Laser Radiation Intensity ◽  
Line Position ◽  
Anti Stokes

The temperature of silicon nanoparticles under laser photo-induced heating is determined from the ratio of the intensities of the Stokes and anti-Stokes components of the Raman scattering. The obtained results of the dependence of nanoparticles temperature on the laser radiation intensity and the temperature dependence of the Raman line position maybe used to determine the optimal regimes of photo-hyperthermia enhanced by silicon nanoparticles for cancer therapy.

scholarly journals Coherent anti-Stokes Raman spectroscopy: Understanding the essentials

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Gombojav O. Ariunbold ◽  
Narangerel Altangerel
Keyword(s):  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Raman Spectra ◽  
Error Function ◽  
Ultrashort Pulses ◽  
Spectroscopic Technique ◽  
Background Suppression ◽  
Closed Form Solutions ◽  
Coherent Raman ◽  
Anti Stokes

AbstractThis paper is a brief overview to coherent anti- Stokes Raman spectroscopic technique and introduces the strengths and barriers to its use all based on the interpretation of simple theoretical formulae. The use of the Gaussian ultrashort pulses is highlighted as a practical elucidatory reconstruction tool of coherent Raman spectra. The paper presents the integral formulae for coherent anti-Stokes and Stokes Raman scattering, and discusses the closed-form solutions, its complex error function, and the delay time formula for enhancement of the inferred pure coherent Raman spectra. As an example, the timeresolved coherent Stokes Raman scattering experimental observations are quantitatively elucidated.Understanding the essentials of coherent Raman spectroscopy, therefore, promotes the importance of a number of experiments including the ones utilizing a broadband excitation with a narrowband delayed probing for successful background suppression.

A Comparative Analysis of the Analytical Capabilities of Coherent Anti-Stokes Raman Spectroscopy (CARS) Relative to Raman Scattering and Absorption Spectroscopy

1977 ◽  
Vol 31 (2) ◽  
pp. 96-103 ◽  
Author(s):  
W. M. Tolles ◽  
R. D. Turner
Keyword(s):  
Raman Spectroscopy ◽  
Raman Scattering ◽  
Absorption Spectroscopy ◽  
Gaseous Mixture ◽  
Minor Component ◽  
Experimental Conditions ◽  
Model Calculations ◽  
Signal Noise ◽  
Noise Ratio ◽  
Anti Stokes

The performance capabilities of coherent anti-stokes Raman spectroscopy (CARS) relative to incoherent Raman scattering and absorption spectroscopy are considered for gases. Four gases are considered for model calculations: hydrogen, nitrogen, oxygen, and carbon monoxide. The signal/noise ratio is estimated for these molecules under a number of assumed experimental conditions. The signal/noise ratios for CARS and Raman scattering scale quite differently with partial pressure and temperature. CARS offers distinct advantages when detecting a major component of a gas mixture at total pressures of considerably less than 1 atm pressure. Raman scattering offers 1 to 2 orders of magnitude greater sensitivity for a signal/noise ratio of unity when detecting a minor component of a gaseous mixture at 1 atm total pressure. These model calculations should be of use when a specific experimental method is to be chosen for spectroscopic examination.

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