Comparative Genome Sequencing of Tomato and Potato: Methods and Analysis

2013 ◽  
pp. 401-420
Keyword(s):  
Genome Sequencing ◽  
Comparative Genome

scholarly journals Comparative Genome Sequencing of Rickettsia rickettsii Strains That Differ in Virulence

2015 ◽  
Vol 83 (4) ◽  
pp. 1568-1576 ◽  
Author(s):  
Tina R. Clark ◽  
Nicholas F. Noriea ◽  
DeAnna C. Bublitz ◽  
Damon W. Ellison ◽  
Craig Martens ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Surface Antigen ◽  
Tandem Repeats ◽  
Spotted Fever ◽  
Protein A ◽  
Rocky Mountain Spotted Fever ◽  
Comparative Genome ◽  
Content Type ◽  
Rickettsia Rickettsii ◽  
Major Surface

Rickettsia rickettsiiis an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. Strains ofR. rickettsiidiffer dramatically in virulence. In a guinea pig model of infection, the severity of disease as assessed by fever response varies from the most virulent, Sheila Smith, to Iowa, which causes no fever. To identify potential determinants of virulence inR. rickettsii, the genomes of two additional strains were sequenced for comparison to known sequences (comparative genome sequencing [CGS]).R. rickettsiiMorgan and R strains were compared to the avirulentR. rickettsiiIowa and virulentR. rickettsiiSheila Smith strains. The Montana strains Sheila Smith and R were found to be highly similar while the eastern strains Iowa and Morgan were most similar to each other. A major surface antigen, rickettsial outer membrane protein A (rOmpA), is severely truncated in the Iowa strain. The region ofompAcontaining 13 tandem repeats was sequenced, revealing only seven shared SNPs (four nonsynonymous) for R and Morgan strains compared to Sheila Smith, with an additional 17 SNPs identified in Morgan. Another major surface antigen and autotransporter, rOmpB, exhibits a defect in processing in the Iowa strain such that the beta fragment is not cleaved. Sequence analysis ofompBreveals identical sequences between Iowa and Morgan strains and between the R and Sheila Smith strains. The number of SNPs and insertions/deletions between sequences of the two Montana strains and the two eastern strains is low, thus narrowing the field of possible virulence factors.

scholarly journals Additional Routes to Staphylococcus aureus Daptomycin Resistance as Revealed by Comparative Genome Sequencing, Transcriptional Profiling, and Phenotypic Studies

PLoS ONE ◽  
2013 ◽  
Vol 8 (3) ◽  
pp. e58469 ◽  
Author(s):  
Yang Song ◽  
Aileen Rubio ◽  
Radheshyam K. Jayaswal ◽  
Jared A. Silverman ◽  
Brian J. Wilkinson
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequencing ◽  
Transcriptional Profiling ◽  
Comparative Genome

scholarly journals Comparative genome sequencing reveals chemotype-specific gene clusters in the toxigenic black mold Stachybotrys

BMC Genomics ◽  
2014 ◽  
Vol 15 (1) ◽  
pp. 590 ◽  
Author(s):  
Jeremy Semeiks ◽  
Dominika Borek ◽  
Zbyszek Otwinowski ◽  
Nick V Grishin
Keyword(s):  
Genome Sequencing ◽  
Gene Clusters ◽  
Specific Gene ◽  
Comparative Genome ◽  
Black Mold
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