Comparative Genome Sequencing for Discovery of Novel Polymorphisms in Bacillus anthracis

Science ◽  
2002 ◽  
Vol 296 (5575) ◽  
pp. 2028-2033 ◽  
Author(s):  
T. D. Read
Keyword(s):  
Bacillus Anthracis ◽  
Genome Sequencing ◽  
Comparative Genome

scholarly journals Comparative Genome Sequencing of Rickettsia rickettsii Strains That Differ in Virulence

2015 ◽  
Vol 83 (4) ◽  
pp. 1568-1576 ◽  
Author(s):  
Tina R. Clark ◽  
Nicholas F. Noriea ◽  
DeAnna C. Bublitz ◽  
Damon W. Ellison ◽  
Craig Martens ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Surface Antigen ◽  
Tandem Repeats ◽  
Spotted Fever ◽  
Protein A ◽  
Rocky Mountain Spotted Fever ◽  
Comparative Genome ◽  
Content Type ◽  
Rickettsia Rickettsii ◽  
Major Surface

Rickettsia rickettsiiis an obligate intracellular pathogen that is the causative agent of Rocky Mountain spotted fever. Strains ofR. rickettsiidiffer dramatically in virulence. In a guinea pig model of infection, the severity of disease as assessed by fever response varies from the most virulent, Sheila Smith, to Iowa, which causes no fever. To identify potential determinants of virulence inR. rickettsii, the genomes of two additional strains were sequenced for comparison to known sequences (comparative genome sequencing [CGS]).R. rickettsiiMorgan and R strains were compared to the avirulentR. rickettsiiIowa and virulentR. rickettsiiSheila Smith strains. The Montana strains Sheila Smith and R were found to be highly similar while the eastern strains Iowa and Morgan were most similar to each other. A major surface antigen, rickettsial outer membrane protein A (rOmpA), is severely truncated in the Iowa strain. The region ofompAcontaining 13 tandem repeats was sequenced, revealing only seven shared SNPs (four nonsynonymous) for R and Morgan strains compared to Sheila Smith, with an additional 17 SNPs identified in Morgan. Another major surface antigen and autotransporter, rOmpB, exhibits a defect in processing in the Iowa strain such that the beta fragment is not cleaved. Sequence analysis ofompBreveals identical sequences between Iowa and Morgan strains and between the R and Sheila Smith strains. The number of SNPs and insertions/deletions between sequences of the two Montana strains and the two eastern strains is low, thus narrowing the field of possible virulence factors.

scholarly journals Bacillus anthracis comparative genome analysis in support of the Amerithrax investigation

2011 ◽  
Vol 108 (12) ◽  
pp. 5027-5032 ◽  
Author(s):  
D. A. Rasko ◽  
P. L. Worsham ◽  
T. G. Abshire ◽  
S. T. Stanley ◽  
J. D. Bannan ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Genome Analysis ◽  
Comparative Genome Analysis ◽  
Comparative Genome

scholarly journals Whole Genome-Sequencing and Phylogenetic Analysis of a Historical Collection of Bacillus anthracis Strains from Danish Cattle

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0134699 ◽  
Author(s):  
Sylviane Derzelle ◽  
Guillaume Girault ◽  
Branko Kokotovic ◽  
Øystein Angen
Keyword(s):  
Phylogenetic Analysis ◽  
Bacillus Anthracis ◽  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome

scholarly journals Genome sequencing of two Bacillus anthracis strains: a virulent strain and a vaccinal strain

2018 ◽  
Vol 49 (1) ◽  
pp. 18-19 ◽  
Author(s):  
Franciele Maboni Siqueira ◽  
Samuel Paulo Cibulski ◽  
Fabiana Quoos Mayer ◽  
David Driemeier ◽  
Saulo Petinatti Pavarini ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Genome Sequencing ◽  
Virulent Strain ◽  
Vaccinal Strain
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