Organization of the intercellular spaces of human palmo-plantar stratum corneum

2002 ◽  
pp. 89-92
Author(s):  
H-M Sheu ◽  
J-C Tsai
Keyword(s):  
Stratum Corneum ◽  
Intercellular Spaces ◽  
Plantar Stratum Corneum

scholarly journals Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis

1996 ◽  
Vol 317 (1) ◽  
pp. 173-177 ◽  
Author(s):  
Michel SIMON ◽  
Marek HAFTEK ◽  
Mireille SEBBAG ◽  
Martine MONTÉZIN ◽  
Elisabeth GIRBAL-NEUHAUSER ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Specific Binding ◽  
Cell Envelope ◽  
Epidermal Differentiation ◽  
Fibrous Matrix ◽  
Cornified Cell Envelope ◽  
Microscopy Analysis ◽  
Covalently Linked ◽  
Cell Envelopes ◽  
Plantar Stratum Corneum

Cornified cell envelope (CE) is generated during the late stages of epidermal differentiation and is made up of proteins covalently linked together by transglutaminases. To determine whether filaggrin is a component of this structure in humans, we analysed highly purified CE from plantar stratum corneum. An immunoelectron microscopy analysis showed specific binding of four different anti-(pro)filaggrin monoclonal antibodies to the surface of the CE, proved previously to be free of non-covalently linked proteins. Moreover, the anti-filaggrin activity of one of the antibodies was absorbed by preincubation with the plantar CE, as determined by ELISA. Convincingly, fragments of CE produced by proteolytic digestion of the structures were stained by this antibody on immunoblots. These data provide direct evidence that filaggrin is a component of CE purified from human plantar stratum corneum. Cross-linking between CE and the filaggrin-containing fibrous matrix may enhance the structural cohesion of the corneocytes and thus the resistance of the stratum corneum.

scholarly journals Purification and Characterization of the Endoglycosidase Heparanase 1 from Human Plantar Stratum Corneum: a Key Enzyme in Epidermal Physiology?

2001 ◽  
Vol 117 (5) ◽  
pp. 1266-1273 ◽  
Author(s):  
Dominique Bernard ◽  
Bruno Méhul ◽  
Caroline Delattre ◽  
Lucie Simonetti ◽  
Agnès Thomas-Collignon ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Purification And Characterization ◽  
Key Enzyme ◽  
Plantar Stratum Corneum

scholarly journals The histochemical distribution of protein bound sulfhydryl groups in human epidermis by the new staining method.

1979 ◽  
Vol 27 (5) ◽  
pp. 942-946 ◽  
Author(s):  
H Ogawa ◽  
A Taneda ◽  
Y Kanaoka ◽  
T Sekine
Keyword(s):  
Cell Membrane ◽  
Stratum Corneum ◽  
Staining Method ◽  
Sulfhydryl Groups ◽  
The Other ◽  
Human Epidermis ◽  
Horny Layer ◽  
Intercellular Spaces ◽  
Sh Groups ◽  
Thiol Reagent

Recently, we synthesized a new fluorescent thiol reagent, N-(7-dimethylamino-4-methylcoumarinyl)-maleimide (DACM) which is nonfluorescent by itself but will react readily with -SH groups to form highly fluorescent addition products. By the use of this reagent, we studied the localization and concentration of -SH groups and S--S linkages in the human epidermis. The distribution of -SH groups in living layers was abundant in cytoplasm but not in nuclei. The fluorescence was concentrated on the cell membrane or intercellular spaces (MIC parts) and was increased at the spino-granular junction. In the horny layer, the fluorescence of the MIC parts appeared brilliantly in the lower layers and decreased gradually. On the other hand, the fluorescence of cytoplasm in keratinized cells in the stratum corneum was faint. The localization of S--S linkages was not a characteristic of the living layers, but appeared abruptly at the junction of living and horny layers. The fluorescence was localized to the MIC parts and disappeared gradually. The distribution of S--S linkages appeared to be very low in the cytoplasm of keratinized cells. No substantial fluorescence was localized on keratohyalin granules even after reduction.

Organization of the intercellular spaces of porcine epidermal and palatal stratum corneum: a quantitative study employing ruthenium tetroxide

1995 ◽  
Vol 279 (2) ◽  
pp. 271-276 ◽  
Author(s):  
D. C. Swartzendruber ◽  
A. Manganaro ◽  
K. C. Madison ◽  
M. Kremer ◽  
P. W. Wertz ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Quantitative Study ◽  
Intercellular Spaces ◽  
Ruthenium Tetroxide

Action of trypsin on human plantar stratum corneum

1980 ◽  
Vol 268 (1) ◽  
pp. 53-64 ◽  
Author(s):  
M. Nicollier ◽  
P. Agache ◽  
J. L. Kienzler ◽  
R. Laurent ◽  
R. Gibey ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Plantar Stratum Corneum

scholarly journals CELL JUNCTIONS IN AMPHIBIAN SKIN

1965 ◽  
Vol 26 (1) ◽  
pp. 263-291 ◽  
Author(s):  
Marilyn G. Farquhar ◽  
George E. Palade
Keyword(s):  
Stratum Corneum ◽  
Membrane Fusion ◽  
Water Soluble ◽  
Cell Junctions ◽  
Physiological Data ◽  
Junctional Complex ◽  
Stratum Granulosum ◽  
Intercellular Spaces ◽  
Outermost Layer ◽  
Zonulae Occludentes

Cell junctions have been investigated in the amphibian epidermis, a stratified squamous epithelium, and compared to those described previously in simple columnar epithelia of mammalian cavitary organs. In adult frogs and toads, and in larvae approaching metamorphosis, belts of membrane fusion or zonulae occludentes of considerable depth are regularly found between adjoining cells of the outermost layer of the stratum corneum, binding the cells together into a continuous, uninterrupted sheet. Another set of occluding zonules appears in the second cornified layer (when such a layer is present), and a third set usually occurs in the outermost layer of the stratum granulosum. Specialized elements described as "modified" and "composite" desmosomes are encountered along the lateral and basal aspects, respectively, of the cornified cells; ordinary desmosomes and maculae occludentes (i.e., spots of membrane fusion) are found in all other strata. The usual 200 A intercellular gap is generally maintained between the cells of the stratum germinativum at the basal ends of the intercellular spaces. Hence, the intercellular spaces of the epidermis form a largely continuous network, closed to the external medium and open to the dermal interstitia. The situation is comparable to that found in columnar epithelia, except that the intercellular spaces are much more extensive, and an extracellular subcompartment (or two) apparently exists in the stratum corneum and between the latter and the stratum granulosum. The last subcompartment is usually filled with a dense substance, probably derived from discharged secretory granules. The tripartite junctional complex characteristic of lumen-lining epithelia (i.e., a zonula occludens followed by a zonula adhaerens, and desmosome) is seen only in early larvae; in adults and in larvae approaching metamorphosis, the occluding zonule is followed directly by a series of modified desmosomes. Interpreted in the light of current physiological data, these findings suggest that the diffusion of water, ions, and small, water-soluble molecules is impeded along the intercellular spaces of the epidermis by zonulae occludentes while it is facilitated from cell to cell within the epidermis by zonulae and maculae occludentes.

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