A chymotrypsin-like proteinase that may be involved in desquamation in plantar stratum corneum

1991 ◽  
Vol 283 (2) ◽  
pp. 108-112 ◽  
Author(s):  
T. Egelrud ◽  
A. Lundstr�m
Keyword(s):  
Stratum Corneum ◽  
Plantar Stratum Corneum

scholarly journals Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis

1996 ◽  
Vol 317 (1) ◽  
pp. 173-177 ◽  
Author(s):  
Michel SIMON ◽  
Marek HAFTEK ◽  
Mireille SEBBAG ◽  
Martine MONTÉZIN ◽  
Elisabeth GIRBAL-NEUHAUSER ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Specific Binding ◽  
Cell Envelope ◽  
Epidermal Differentiation ◽  
Fibrous Matrix ◽  
Cornified Cell Envelope ◽  
Microscopy Analysis ◽  
Covalently Linked ◽  
Cell Envelopes ◽  
Plantar Stratum Corneum

Cornified cell envelope (CE) is generated during the late stages of epidermal differentiation and is made up of proteins covalently linked together by transglutaminases. To determine whether filaggrin is a component of this structure in humans, we analysed highly purified CE from plantar stratum corneum. An immunoelectron microscopy analysis showed specific binding of four different anti-(pro)filaggrin monoclonal antibodies to the surface of the CE, proved previously to be free of non-covalently linked proteins. Moreover, the anti-filaggrin activity of one of the antibodies was absorbed by preincubation with the plantar CE, as determined by ELISA. Convincingly, fragments of CE produced by proteolytic digestion of the structures were stained by this antibody on immunoblots. These data provide direct evidence that filaggrin is a component of CE purified from human plantar stratum corneum. Cross-linking between CE and the filaggrin-containing fibrous matrix may enhance the structural cohesion of the corneocytes and thus the resistance of the stratum corneum.

scholarly journals Purification and Characterization of the Endoglycosidase Heparanase 1 from Human Plantar Stratum Corneum: a Key Enzyme in Epidermal Physiology?

2001 ◽  
Vol 117 (5) ◽  
pp. 1266-1273 ◽  
Author(s):  
Dominique Bernard ◽  
Bruno Méhul ◽  
Caroline Delattre ◽  
Lucie Simonetti ◽  
Agnès Thomas-Collignon ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Purification And Characterization ◽  
Key Enzyme ◽  
Plantar Stratum Corneum

Action of trypsin on human plantar stratum corneum

1980 ◽  
Vol 268 (1) ◽  
pp. 53-64 ◽  
Author(s):  
M. Nicollier ◽  
P. Agache ◽  
J. L. Kienzler ◽  
R. Laurent ◽  
R. Gibey ◽  
...  
Keyword(s):  
Stratum Corneum ◽  
Plantar Stratum Corneum

30-kDa Trypsin-like Proteases in the Plantar Stratum Corneum

1997 ◽  
Vol 24 (8) ◽  
pp. 504-509 ◽  
Author(s):  
Cui Chang-Yi ◽  
Masae Takahashi ◽  
Tadashi Tezuka
Keyword(s):  
Stratum Corneum ◽  
Plantar Stratum Corneum
Sign in / Sign up

Export Citation Format

Share Document