Signal Transduction and Cytokine Expression in Particulate Matter (PM)-Induced Airway Remodeling

2005 ◽  
pp. 233-258
Author(s):  
Dianne Walters ◽  
James Bonner
Keyword(s):  
Signal Transduction ◽  
Particulate Matter ◽  
Airway Remodeling ◽  
Cytokine Expression

scholarly journals Lactic Acid Bacteria Ameliorate Diesel Exhaust Particulate Matter-Exacerbated Allergic Inflammation in a Murine Model of Asthma

Life ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 260
Author(s):  
Sun Woo Jin ◽  
Gi Ho Lee ◽  
Min Jung Jang ◽  
Gyeong Eun Hong ◽  
Jae Young Kim ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Particulate Matter ◽  
Lactic Acid Bacteria ◽  
Diesel Exhaust ◽  
Immunoglobulin E ◽  
Therapeutic Potential ◽  
Airway Remodeling ◽  
Cell Hyperplasia ◽  
Diesel Exhaust Particulate ◽  
Exhaust Particulate

Several air pollution components such as sulfur dioxide, ozone, nitrogen dioxide, and diesel exhaust particulate matter (DEPM) have been linked to the development of asthma. In this study, we investigated the therapeutic potential of three lactic acid bacteria species, Lactobacillus plantarum GREEN CROSS Wellbeing (GCWB)1001, Pediococcus acidilactici GCWB1085, and Lactobacillus rhamnosus GCWB1156, in preventing DEPM-exacerbated asthma in mice. BALB/c mice were first sensitized with ovalbumin (OVA) and were either challenged with OVA or DEPM (DEPM-exacerbated asthma model) by intranasal instillation. All three strains showed no hemolytic activity, suggesting a good safety profile. Oral administration of lactic acid bacteria reduced OVA + DEPM-induced inflammatory infiltration, goblet cell hyperplasia, airway remodeling, and the levels of proinflammatory cytokines and chemokines in bronchoalveolar lavage fluid (BALF). The probiotics also attenuated OVA + DEPM-induced immunoglobulin E (IgE) levels in serum and in BALF, and significantly reduced caspase-3 activity, total collagen level, and matrix metalloproteinase (MMP)-9 activity. In conclusion, lactic acid bacteria such as L. plantarum GCWB1001, P. acidilactici GCWB1085, and L. rhamnosus treatment in mice with asthma showed significant efficacy in preventing lung inflammation exacerbated by DEPM administration.

Airway remodeling in chronic alcohol C57BL/6 mice with inhalation co-exposure to combustion-generated particulate matter

Alcohol ◽  
2012 ◽  
Vol 46 (2) ◽  
pp. 178
Author(s):  
P.T. Thevenot ◽  
J. Saravia ◽  
T. Ahlert ◽  
M.A. Kelly ◽  
T.R. Dugas ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Airway Remodeling ◽  
Chronic Alcohol

Rimexolone inhibits proliferation, cytokine expression and signal transduction of human CD4+ T-cells

2010 ◽  
Vol 131 (1) ◽  
pp. 24-32 ◽  
Author(s):  
Cornelia M. Spies ◽  
Timo Gaber ◽  
Martin Hahne ◽  
Lydia Naumann ◽  
Robert Tripmacher ◽  
...  
Keyword(s):  
Signal Transduction ◽  
T Cells ◽  
Cd4 T Cells ◽  
Cytokine Expression

ID: 129: PARTICULATE MATTER DISRUPTS ENDOTHELIAL CELL PERMEABILITY VIA GAP JUNCTION PROTEIN

2016 ◽  
Vol 64 (4) ◽  
pp. 970.2-971
Author(s):  
X Wu ◽  
X Xu ◽  
JG Garcia ◽  
T Wang
Keyword(s):  
Signal Transduction ◽  
Particulate Matter ◽  
Endothelial Cell ◽  
Gap Junction ◽  
Cell Permeability ◽  
Mrna Levels ◽  
Barrier Integrity ◽  
Junction Protein ◽  
Gap Junction Protein ◽  
Cx43 Mrna

IntroductionParticulate matter (PM) is significantly associated with cardiopulmonary morbidity and mortality. We previously demonstrated that PM induces endothelial barrier disruption via reactive oxygen species (ROS)-dependent mechanisms. This study is focused on characterization of PM-regulated endothelial dysfunction via connexin43 (Cx43), a Gap junction protein. Gap junction is designated as intercellular channel which allows cells to communicate with each other, share nutrients, and transfer chemical or electrical signals, in turn, enables cells in a tissue to function in a coordinated manner.Methods and ResultsCx43 protein levels were evaluated by western blotting, and band density quantified using MyImageAnalysis. Real-time PCR was conducted to determine Cx43 mRNA levels. Human pulmonary artery endothelial cell (EC) barrier function was measured using the electrical cell-substrate impedance sensing (ECIS) system (Applied Biophysics) that provides a readout of transendothelial electrical resistance (TER). PM sample (0.1–0.3 µm of aerodynamic diameter) was collected (April of 2005) from the Ft. McHenry Tunnel, Baltimore, MD using a high-volume cyclone collector. PM (100 µg/ml) induced time-dependent increases in EC Cx43 mRNA levels (∼5 fold increase at 4 hr) and protein expression which was attenuated by N-acetyl-cysteine (NAC, 5 mM, 1 hr pretreatment), an ROS scavenger. Unlike Cx43, Cx37, another connexin expressed in ECs, remained unaltered by PM challenge. In addition, EC pretreatment with a Cx43 inhibitor, connexin-mimetic peptide Gap27 (500 µM, 2 hr pretreatment), significantly attenuated PM-reduced TER reduction by 45%, suggesting a central role of Cx43 in PM-induced lung EC barrier integrity disruption and signal transduction.ConclusionsOur results suggest Cx43 as a key and novel participant in PM-mediated signal transduction that results in loss of vascular barrier integrity. Cx43 may serve as a therapeutic target in PM-induced cardiopulmonary toxicities.

scholarly journals MicroRNA-145 down-regulates mucin 5AC to alleviate airway remodeling and targets EGFR to inhibit cytokine expression

Oncotarget ◽  
2017 ◽  
Vol 8 (28) ◽  
pp. 46312-46325 ◽  
Author(s):  
Zhe Cheng ◽  
Ling-Ling Dai ◽  
Xi Wang ◽  
Liu-Qun Jia ◽  
Xiao-Gang Jing ◽  
...  
Keyword(s):  
Airway Remodeling ◽  
Cytokine Expression

AnIn Vitroalveolar macrophage assay for the assessment of inflammatory cytokine expression induced by atmospheric particulate matter

2014 ◽  
Vol 30 (7) ◽  
pp. 836-851 ◽  
Author(s):  
Zana Sijan ◽  
Dagmara S. Antkiewicz ◽  
Jongbae Heo ◽  
Norman Y. Kado ◽  
James J. Schauer ◽  
...  
Keyword(s):  
Particulate Matter ◽  
Inflammatory Cytokine ◽  
Cytokine Expression ◽  
Atmospheric Particulate Matter ◽  
Atmospheric Particulate
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