The Use of Coumarins as Environmentally-Sensitive Fluorescent Probes of Heterogeneous Inclusion Systems

Spectroscopy ◽  
2011 ◽  
pp. 278-313
Author(s):  
Brian Wagner
Keyword(s):  
Fluorescent Probes ◽  
Environmentally Sensitive

Discovery of Nonpeptide, Environmentally Sensitive Fluorescent Probes for Imaging p53-MDM2 Interactions in Living Cell Lines and Tissue Slice

2020 ◽  
Vol 92 (3) ◽  
pp. 2642-2648 ◽  
Author(s):  
Gaopan Dong ◽  
Shipeng He ◽  
Xiaojun Qin ◽  
Tingting Liu ◽  
Yan Jiang ◽  
...  
Keyword(s):  
Cell Lines ◽  
Fluorescent Probes ◽  
Living Cell ◽  
Tissue Slice ◽  
Environmentally Sensitive

scholarly journals A General Method to Develop Highly Environmentally Sensitive Fluorescent Probes and AIEgens

2021 ◽  
pp. 2104609
Author(s):  
Rong Miao ◽  
Jing Li ◽  
Chao Wang ◽  
Xuefeng Jiang ◽  
Ying Gao ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Environmentally Sensitive ◽  
General Method

scholarly journals Selected peptide-based fluorescent probes for biological applications

2020 ◽  
Vol 16 ◽  
pp. 2971-2982
Author(s):  
Debabrata Maity
Keyword(s):  
Fluorescent Probes ◽  
Direct Detection ◽  
Aqueous Media ◽  
Live Cells ◽  
Biological Applications ◽  
Site Specific ◽  
Specific Manner ◽  
Environmentally Sensitive ◽  
Living Organisms ◽  
A Site

To understand the molecular interactions, present in living organisms and their environments, chemists are trying to create novel chemical tools. In this regard, peptide-based fluorescence techniques have attracted immense interest. Synthetic peptide-based fluorescent probes are advantageous over protein-based sensors, since they are synthetically accessible, more stable, and can be easily modified in a site-specific manner for selective biological applications. Peptide receptors labeled with environmentally sensitive/FRET fluorophores have allowed direct detection/monitoring of biomolecules in aqueous media and in live cells. In this review, key peptide-based approaches for different biological applications are presented.

Synthesis and photophysical evaluation of new fluorescent 7-arylethynyl-7-deazaadenosine analogs

2018 ◽  
Vol 96 (12) ◽  
pp. 1093-1100 ◽  
Author(s):  
Augusto Matarazzo ◽  
Justin Brow ◽  
Robert H.E. Hudson
Keyword(s):  
Fluorescent Probes ◽  
Emission Intensity ◽  
Spectral Properties ◽  
Fluorescence Emission ◽  
Coupling Reaction ◽  
Cross Coupling ◽  
Quantum Yields ◽  
Cross Coupling Reaction ◽  
Fluorescence Emission Intensity ◽  
Environmentally Sensitive

Three new fluorescent 7-deaza-2′-deoxyadenosine analogs were synthesized via the Sonogashira cross-coupling reaction of 7-iodo-7-deaza-2′-deoxyadenosine with 1-ethynylpyrene, 2-ethynyl-6-methoxynaphthalene, and 9-ethynylphenanthrene. The spectral properties of these analogs were evaluated in dioxane, EtOH, and H2O to determine their potential for use as environmentally sensitive fluorescent probes. All three analogs displayed large solvatofluorochromicity in H2O, relative to their emission wavelengths in dioxane or EtOH. Moreover, all three analogs exhibited microenvironmental sensitivity of their fluorescence emission intensity, being moderate to high quantum yields in dioxane and EtOH and significantly lower in H2O. Various attempts to perform domino cross-coupling and annuation reactions on 7-deaza-7-alkynyladenine derivatives to form a new fused tricyclic adenine analog were unsuccessful.

Confocal microscopy of the cytoskeleton in living plant cells following microinjection of fluorescent probes

1993 ◽  
Vol 51 ◽  
pp. 130-131
Author(s):  
Ann Cleary
Keyword(s):  
Cell Division ◽  
Fluorescent Probes ◽  
Actin Filaments ◽  
Intracellular Transport ◽  
Cell Structure ◽  
Plant Cells ◽  
Cell Plate ◽  
Cell Cortex ◽  
Living Plant ◽  
Rhodamine Phalloidin

Microinjection of fluorescent probes into living plant cells reveals new aspects of cell structure and function. Microtubules and actin filaments are dynamic components of the cytoskeleton and are involved in cell growth, division and intracellular transport. To date, cytoskeletal probes used in microinjection studies have included rhodamine-phalloidin for labelling actin filaments and fluorescently labelled animal tubulin for incorporation into microtubules. From a recent study of Tradescantia stamen hair cells it appears that actin may have a role in defining the plane of cell division. Unlike microtubules, actin is present in the cell cortex and delimits the division site throughout mitosis. Herein, I shall describe actin, its arrangement and putative role in cell plate placement, in another material, living cells of Tradescantia leaf epidermis.The epidermis is peeled from the abaxial surface of young leaves usually without disruption to cytoplasmic streaming or cell division. The peel is stuck to the base of a well slide using 0.1% polyethylenimine and bathed in a solution of 1% mannitol +/− 1 mM probenecid.

Live Confocal Analysis of Fertilization and Early Development

2001 ◽  
Vol 7 (S2) ◽  
pp. 1012-1013
Author(s):  
Uyen Tram ◽  
William Sullivan
Keyword(s):  
Drosophila Melanogaster ◽  
Embryonic Development ◽  
Real Time ◽  
Early Development ◽  
Fluorescent Probes ◽  
Primary Defect ◽  
Fluorescent Analysis ◽  
Dynamic Event ◽  
Enormous Amount ◽  
Fluorescent Studies

Embryonic development is a dynamic event and is best studied in live animals in real time. Much of our knowledge of the early events of embryogenesis, however, comes from immunofluourescent analysis of fixed embryos. While these studies provide an enormous amount of information about the organization of different structures during development, they can give only a static glimpse of a very dynamic event. More recently real-time fluorescent studies of living embryos have become much more routine and have given new insights to how different structures and organelles (chromosomes, centrosomes, cytoskeleton, etc.) are coordinately regulated. This is in large part due to the development of commercially available fluorescent probes, GFP technology, and newly developed sensitive fluorescent microscopes. For example, live confocal fluorescent analysis proved essential in determining the primary defect in mutations that disrupt early nuclear divisions in Drosophila melanogaster. For organisms in which GPF transgenics is not available, fluorescent probes that label DNA, microtubules, and actin are available for microinjection.

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