Fundamentals and Perspectives of Molecular Imprinting in Sensor Applications

Background: Until now, several methods such as spectroscopic methods and chromatographic techniques have been developed for the determination of biomolecules, drug or heavy metals. Nevertheless, the crucial interference problems are present in these methods. Due to these reasons, more sensitive, favorable portability, low-cost, simple and selective sensors based on nanocomposites are needed in terms of health safety. In the development of electrochemical nanosensor, the nanomaterials such as graphene/graphene oxide, carbon and carbon nitride nanotubes are utilized to improve the sensitivity. Objective: The nanomaterials such as graphene/graphene oxide, carbon and carbon nitride nanotubes have important advantages such as high surface area, electrical conductivity, thermal and mechanical stability. Hence, we presented the highly selective methods for sensitive sensor applications by molecular imprinting technology in literature. This technology is a polymerization method around target molecule. This method provides the specific cavities to analyte molecule on the polymer surface. Hence, the selective sensor is easily created for biomedical and other applications. Novel electrochemical sensors based on nanocomposite whose surface is coated with Molecular Imprinting Polymer (MIP) are developed and then applied to the selective and sensitive detection in this study. Until now, we have presented several reports about nanocomposite based sensor with MIP.

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Feasibility study on molecularly imprinted assays for biomedical diagnostics

Sensor Review ◽

10.1108/sr-08-2018-0211 ◽

2019 ◽

Vol 39 (6) ◽

pp. 862-873 ◽

Cited By ~ 1

Author(s):

Qian Yee Ang ◽

Siew Chun Low

Keyword(s):

Molecular Imprinting ◽

Optical Sensing ◽

Building Blocks ◽

Molecularly Imprinted ◽

Content Type ◽

Sensor Applications ◽

Biomedical Diagnostics ◽

Underlying Mechanisms ◽

Open Question ◽

Medical Interest

Purpose Molecularly imprinted polymers (MIPs) have aroused focus in medicinal chemistry in recent decades, especially for biomedical applications. Considering the exceptional abilities to immobilize any guest of medical interest (antibodies, enzymes, etc.), MIPs is attractive to substantial research efforts in complementing the quest of biomimetic recognition systems. This study aims to review the key-concepts of molecular imprinting, particularly emphasizes on the conformational adaptability of MIPs beyond the usual description of molecular recognition. The optimal morphological integrity was also outlined in this review to acknowledge the successful sensing activities by MIPs. Design/methodology/approach This review highlighted the fundamental mechanisms and underlying challenges of MIPs from the preparation stage to sensor applications. The progress of electrochemical and optical sensing using molecularly imprinted assays has also been furnished, with the evolvement of molecular imprinting as a research hotspot. Findings The lack of standard synthesis protocol has brought about an intriguing open question in the selection of building blocks that are biocompatible to the imprint species of medical interest. Thus, in this paper, the shortcomings associated with the applications of MIPs in electrochemical and optical sensing were addressed using the existing literature besides pointing out possible solutions. Future perspectives in the vast development of MIPs also been postulated in this paper. Originality/value The present review intends to furnish the underlying mechanisms of MIPs in biomedical diagnostics, with the aim in electrochemical and optical sensing while hypothesizing on future possibilities.

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Molecular Imprinting of Macromolecules for Sensor Applications

Sensors ◽

10.3390/s17040898 ◽

2017 ◽

Vol 17 (4) ◽

pp. 898 ◽

Cited By ~ 54

Author(s):

Yeşeren Saylan ◽

Fatma Yilmaz ◽

Erdoğan Özgür ◽

Ali Derazshamshir ◽

Handan Yavuz ◽

...

Keyword(s):

Molecular Imprinting ◽

Sensor Applications

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Molecular Imprinting for Sensor Applications

Molecular Recognition and Polymers ◽

10.1002/9780470384053.ch15 ◽

2008 ◽

pp. 395-429 ◽

Cited By ~ 6

Author(s):

Xiangyang Wu ◽

Ken D. Shimizu

Keyword(s):

Molecular Imprinting ◽

Sensor Applications

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Fundamentals of Molecular Imprinting and Sensor Applications

Handbook of Molecular Imprinting ◽

10.1201/b13062-2 ◽

2012 ◽

pp. 1-1

Keyword(s):

Molecular Imprinting ◽

Sensor Applications

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The Role of the Initiator System in the Synthesis of Acidic Multifunctional Nanoparticles Designed for Molecular Imprinting of Proteins

Periodica Polytechnica Chemical Engineering ◽

10.3311/ppch.15414 ◽

2020 ◽

Vol 65 (1) ◽

pp. 28-41

Author(s):

Marwa Aly Ahmed ◽

Júlia Erdőssy ◽

Viola Horváth

Keyword(s):

Acrylic Acid ◽

Binding Affinity ◽

Molecular Imprinting ◽

Low Temperatures ◽

Ammonium Persulfate ◽

Sodium Bisulfite ◽

Multifunctional Nanoparticles ◽

High Affinity ◽

Initiator System

Multifunctional nanoparticles have been shown earlier to bind certain proteins with high affinity and the binding affinity could be enhanced by molecular imprinting of the target protein. In this work different initiator systems were used and compared during the synthesis of poly (N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) nanoparticles with respect to their future applicability in molecular imprinting of lysozyme. The decomposition of ammonium persulfate initiator was initiated either thermally at 60 °C or by using redox activators, namely tetramethylethylenediamine or sodium bisulfite at low temperatures. Morphology differences in the resulting nanoparticles have been revealed using scanning electron microscopy and dynamic light scattering. During polymerization the conversion of each monomer was followed in time. Striking differences were demonstrated in the incorporation rate of acrylic acid between the tetramethylethylenediamine catalyzed initiation and the other systems. This led to a completely different nanoparticle microstructure the consequence of which was the distinctly lower lysozyme binding affinity. On the contrary, the use of sodium bisulfite activation resulted in similar nanoparticle structural homogeneity and protein binding affinity as the thermal initiation.

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Hybrid Micro-Optical Sensors via Sol-Gel Soft Lithography

MRS Proceedings ◽

10.1557/proc-628-cc9.3 ◽

2000 ◽

Vol 628 ◽

Cited By ~ 1

Author(s):

Mark A. Clarner ◽

Michael J. Lochhead

Keyword(s):

Optical Sensors ◽

Soft Lithography ◽

Sol Gel ◽

Silica Gels ◽

Ph Sensing ◽

Ambient Conditions ◽

Sensor Applications ◽

Patterned Structures ◽

Biological Functionality ◽

Indicator Dyes

ABSTRACTOrganically modified silica gels and dye-doped silica gels have been patterned into micrometer-scale structures on a substrate using micro molding in capillaries (MIMIC). This approach is from a class of elastomeric stamping and molding techniques collectively known as soft lithography. Soft lithography and sol-gel processing share attractive features in that they are relatively benign processes performed at ambient conditions, which makes both techniques compatible with a wide variety of organic molecules, molecular assemblies, and biomolecules. The combination of sol-gel and soft lithography, therefore, holds enormous promise as a tool for microfabrication of materials with optical, chemical, or biological functionality that are not readily patterned with conventional methods. This paper describes our investigation of micro-patterned organic-inorganic hybrid materials containing indicator dyes for microfluidic sensor applications. Reversible colorimetric pH sensing via entrapped reagents is demonstrated in a prototype microfluidic sensor element. Patterned structures range from one to tens of micrometers in cross-section and are up to centimeters in length. Fundamental chemical processing issues associated with mold filling, cracking and sensor stability are discussed.

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