Human Neural Stem Cells Genetically Modified to Overexpress Akt1 Provide Neuroprotection and Functional Improvement in Mouse Stroke Model

2011 ◽  
pp. 299-320 ◽  
Author(s):  
Hong Lee ◽  
Mi Kim ◽  
Hee Kim ◽  
Seung Kim
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Genetically Modified ◽  
Functional Improvement ◽  
Stroke Model ◽  
Human Neural Stem Cells

Brain Transplantation of Immortalized Human Neural Stem Cells Promotes Functional Recovery in Mouse Intracerebral Hemorrhage Stroke Model

Stem Cells ◽  
2007 ◽  
Vol 25 (5) ◽  
pp. 1204-1212 ◽  
Author(s):  
Hong J. Lee ◽  
Kwang S. Kim ◽  
Eun J. Kim ◽  
Hyun B. Choi ◽  
Kwang H. Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Intracerebral Hemorrhage ◽  
Neural Stem Cells ◽  
Functional Recovery ◽  
Stroke Model ◽  
Human Neural Stem Cells

scholarly journals Transplantation of human neural stem cells into the stroke model

Nosotchu ◽  
2002 ◽  
Vol 24 (3) ◽  
pp. 355-358
Author(s):  
Osamu Honmou ◽  
Shinichi Oka ◽  
Masanori Sasaki ◽  
Akira Takahashi ◽  
Atsushi Tanooka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Stroke Model ◽  
Human Neural Stem Cells

scholarly journals Exosomes derived from human neural stem cells stimulated by interferon gamma improve therapeutic ability in ischemic stroke model

2020 ◽  
Vol 24 ◽  
pp. 435-445 ◽  
Author(s):  
Guilong Zhang ◽  
Zhihan Zhu ◽  
Hong Wang ◽  
Yongbo Yu ◽  
Wanghao Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Ischemic Stroke ◽  
Neural Stem Cells ◽  
Interferon Gamma ◽  
Stroke Model ◽  
Human Neural Stem Cells

scholarly journals mRNA-Driven Generation of Transgene-Free Neural Stem Cells from Human Urine-Derived Cells

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1043 ◽  
Author(s):  
Phil Jun Kang ◽  
Daryeon Son ◽  
Tae Hee Ko ◽  
Wonjun Hong ◽  
Wonjin Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Urine ◽  
Neurological Disorders ◽  
Therapeutic Potential ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Patient Specific ◽  
Human Neural Stem Cells

Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteristics, global gene expression, and potential to differentiate into functional neurons, astrocytes, and oligodendrocytes. Moreover, iNSC colonies were observed within eight days under optimized conditions, and no teratomas formed in vivo, implying the absence of pluripotent cells. This study proposes an approach to generate transplantable iNSCs that can be broadly applied for neurological disorders in a safe, efficient, and patient-specific manner.

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