- Inverse Relationship between Urinary Retinol-Binding Protein, Beta-2-Microglobulin, and Blood Manganese Levels in School-Age Children from an E-Waste Recycling Town

2012 ◽  
pp. 204-213
Keyword(s):  
Inverse Relationship ◽  
Binding Protein ◽  
Waste Recycling ◽  
Retinol Binding Protein ◽  
School Age Children ◽  
School Age ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Blood Manganese

Measurement of urinary retinol-binding protein by enzyme-linked immunosorbent assay, and its application to detection of tubular proteinuria.

1986 ◽  
Vol 32 (10) ◽  
pp. 1863-1866 ◽  
Author(s):  
M D Topping ◽  
H W Forster ◽  
C Dolman ◽  
C M Luczynska ◽  
A M Bernard
Keyword(s):  
Immunosorbent Assay ◽  
Binding Protein ◽  
Geometric Mean ◽  
Retinol Binding Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Tubular Proteinuria ◽  
Chi Square ◽  
Beta 2 ◽  
Beta 2 Microglobulin ◽  
Chi Square Analysis

Abstract An enzyme-linked immunosorbent assay (ELISA) for urinary retinol-binding protein (RBP) has been developed and compared with urinary beta 2-microglobulin for the detection of tubular proteinuria. The assay has a working range of 10 to 250 micrograms of RBP per liter of urine. The within-assay CV was 3.2-7.1%, the between-assay CV 12.5%. A control population of 118 male subjects gave a geometric mean urinary RBP concentration of 7.7 micrograms per millimole of creatinine and a 95th centile of 22 micrograms per millimole of creatinine. Comparison of urinary RBP and beta 2-microglobulin concentrations in 80 control subjects and 117 subjects exposed to cadmium fumes gave correlations of r = 0.59 and 0.91, respectively. Of the 117 subjects exposed to cadmium fumes, 103 gave both RBP and beta 2-microglobulin concentrations on the same side of the upper 95th centile values of 22 and 38 micrograms per millimole of creatinine for RBP and beta 2-microglobulin respectively (Chi-square analysis p less than 0.001), demonstrating that RBP and beta 2-microglobulin detect tubular proteinuria with equal sensitivity and specificity. ELISA and an established latex immunoassay gave well-correlated results.

Assessment of urinary retinol-binding protein as an index of proximal tubular injury.

1987 ◽  
Vol 33 (6) ◽  
pp. 775-779 ◽  
Author(s):  
A M Bernard ◽  
A A Vyskocil ◽  
P Mahieu ◽  
R R Lauwerys
Keyword(s):  
Urinary Excretion ◽  
Binding Protein ◽  
Retinol Binding Protein ◽  
Tubular Damage ◽  
Tubular Injury ◽  
Renal Tubular Damage ◽  
Acid Urine ◽  
Beta 2 ◽  
Renal Tubular ◽  
Beta 2 Microglobulin

Abstract The urinary excretion of retinol-binding protein (RBP), beta 2-microglobulin (beta 2-m), and beta-N-acetyl-D-glucosaminidase was monitored in patients with renal tubular damage secondary to multiple injuries, rhabdomyolysis, antibiotic treatment, or poisoning by various chemicals such as solvents, heavy metals, or pesticides. In almost all cases, RBP proved to be a more sensitive index of renal tubular damage than was beta-N-acetyl-D-glucosaminidase and, being more stable in acid urine, a more practical analyte to measure than was beta 2-m. We corroborated this finding by studying the relationships between these three analytes in more than 150 patients. On the average, an increase in the urinary excretion of beta-N-acetyl-D-glucosaminidase becomes detectable when urinary RBP already exceeds the normal value by 50- to 100-fold. In urines with pH greater than 6, RBP and beta 2-m concentrations are well correlated (r = 0.93, n = 150), beta 2-m tending to be more frequently positive (i.e., greater than 311 micrograms/L). But in urines with pH less than 6 (about 30-40% of the samples), the RBP/beta 2-m concentration ratio increases as pH decreases, up to 500 in some patients with massive tubular injury. Because the renal uptake of proteins involves a saturable process, the urinary excretion of RBP, like that of beta 2-m, specifically reflects the reabsorption capacity of proximal tubules only when the glomerular filtration rate is normal or slightly impaired (i.e., serum creatinine less than 20 mg/L). Under these conditions the determination of RBP protein in urine appears the most appropriate test when early detection of tubular injury is desirable.

Early Detection of Renal Dysfunction in β Thalassemia with Focus on Novel Biomarkers

2020 ◽  
Author(s):  
Mozhgan Hashemieh
Keyword(s):  
Renal Dysfunction ◽  
Early Recognition ◽  
Binding Protein ◽  
Kidney Injury ◽  
Thalassemia Major ◽  
Retinol Binding Protein ◽  
Fatty Acid Binding ◽  
Novel Biomarkers ◽  
Beta 2 Microglobulin ◽  
Kidney Injury Molecule 1

Improved survival among transfusion dependent thalassemia patients in recent years has led to the manifestation of morbidities such as renal dysfunction. Renal injury is still an underestimated complication in β thalassemia major patients. Chronic anemia, iron overload due to repeated transfusion, and specific iron chelators are the main factors in pathogenesis of renal dysfunction in β thalassemia. Early identification of this morbidity allows us to delay the progression of kidney damage and therefore reduce renal impairment. In recent decades , novel biomarkers for early recognition of renal dysfunction have been studied in thalassemic patients, such as cystatin C, beta 2 microglobulin , alpha 1 microglobulin, N-acetyl beta-D-glucosaminidase (NAG), neutrophil gelatinase associated lipocaline (NGAL) , kidney injury molecule 1 (KIM-1) , liver type fatty acid binding protein (L-FABP), and retinol binding protein (RBP). In this review, renal aspects of thalassemia with focus on novel biomarkers were discussed.

The Effect of Blood Manganese Level on Neurobehavioral Function in Early School Age Children

Epidemiology ◽  
2011 ◽  
Vol 22 ◽  
pp. S283
Author(s):  
Soo Eun Chung ◽  
Hae-Kwan Cheong ◽  
Ho-Jang Kwon ◽  
Mina Ha ◽  
Dongmug Kang ◽  
...  
Keyword(s):  
School Age Children ◽  
School Age ◽  
Neurobehavioral Function ◽  
Early School ◽  
Early School Age ◽  
Blood Manganese ◽  
Manganese Level

Immunonephelometric determination of retinol-binding protein in serum and urine.

1983 ◽  
Vol 29 (5) ◽  
pp. 853-856 ◽  
Author(s):  
M T Parviainen ◽  
P Ylitalo
Keyword(s):  
Binding Protein ◽  
Retinol Binding Protein ◽  
Good Precision ◽  
Serum Samples ◽  
Detection Range ◽  
Analytical Recovery ◽  
Beta 2 Microglobulin ◽  
Urine Specimens ◽  
Serum And Urine

Abstract An immunonephelometric method developed for measurement of retinol-binding protein (RBP) in serum and urine can detect it in concentrations of about 30 micrograms/L, which is in the lower limit of its normal concentration in urine (range 0-0.56 mg/L; mean +/- SD 0.19 +/- 0.15; n = 44). Urinary RBP was increased (range 0.93-29.5 mg/L) in all of 25 urine specimens from 13 subjects being treated with aminoglycoside (tobramycin). Urinary excretion of RBP was correlated (r = 0.83) with the excretion of beta 2-microglobulin. The within-assay and day-to-day precision (CV) was determined over the detection range of 0.03-8 mg/L. Within these limits the corresponding CVs varied from 4 to 27% and from 8 to 30%, respectively. The method had fairly good precision within the optimal measuring range of approximately 0.4 to 4.5 mg/L for both urine and 20-fold diluted serum samples. For various RBP concentrations our analytical recovery was 89-114% of added RBP. Results by this method correlated well (r = 0.96, n = 24) with those by a radial immunodiffusion method.

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