Identification of Fungal Pathogenicity Genes by Agrobacterium tumefaciens-Mediated Transformation

2012 ◽  
pp. 1-19 ◽  
Author(s):  
Karunakaran Maruthachalam ◽  
Junhyun Jeon ◽  
Yong-Hwan Lee ◽  
Krishna Subbarao
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Fungal Pathogenicity ◽  
Pathogenicity Genes

scholarly journals Fungal pathogenicity genes in the age of ‘omics’

2010 ◽  
Vol 12 (5) ◽  
pp. 507-514 ◽  
Author(s):  
ANGELA P. VAN DE WOUW ◽  
BARBARA J. HOWLETT
Keyword(s):  
Fungal Pathogenicity ◽  
Pathogenicity Genes

scholarly journals Differentiation of Phytopathogenic agrobacterium spp.

2011 ◽  
Vol 26 (3) ◽  
pp. 245-253
Author(s):  
Nemanja Kuzmanovic ◽  
Milan Ivanovic ◽  
Andjelka Calic ◽  
Katarina Gasic ◽  
Aleksa Obradovic
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Molecular Methods ◽  
Duplex Pcr ◽  
Pathogenicity Genes ◽  
Standardized Protocol ◽  
Physiological Tests ◽  
Test Plants

Due to the difficulties in differentiation of phytopathogenic Agrobacterium spp. and lack of a standardized protocol, we carried out selection and evaluation of suitable methods based on the bacterial physiological, genetic and pathogenic properties. Strains of Agrobacterium tumefaciens, A. rhizogenes and A. vitis were differentiated using standard bacteriological and molecular methods. The biochemical and physiological tests confirmed authenticity of the strains. Two duplex PCR methods were conducted with four different primer pairs. In all strains, presence of plasmid virD2 and virC pathogenicity genes was detected. Chromosomal pehA gene was determined in A. vitis strain. Pathogenicity was confirmed on carrot slices and young plants of tomato and sunflower. Strains of A. tumefaciens and A. vitis were pathogenic on all test plants, while strain of A. rhizogenes induced characteristic symptoms only on carrot slices. The tests used in this study provided reliable discrimination between the three species and confirmed their identity as tumorigenic (Ti) Agrobacterium tumefaciens and A. vitis, and rhizogenic (Ri) A. rhizogenes.

Genetic transformation of Ginkgo biloba by Agrobacterium tumefaciens

2000 ◽  
Vol 108 (4) ◽  
pp. 413-419
Author(s):  
Patricia Dupré ◽  
Jerôme Lacoux ◽  
Godfrey Neutelings ◽  
Dominique Mattar-Laurain ◽  
Marc-André Fliniaux ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Genetic Transformation ◽  
Ginkgo Biloba
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