Chromatographic Separation and NMR

2012 ◽  
pp. 93-138 ◽  
Author(s):  
Nina Gonnella
Keyword(s):  
Chromatographic Separation

DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

1962 ◽  
Vol 41 (2) ◽  
pp. 234-246 ◽  
Author(s):  
H. J. van der Molen
Keyword(s):  
Infrared Spectrum ◽  
Quantitative Determination ◽  
Acid Hydrolysis ◽  
Chromatographic Separation ◽  
Pure Form ◽  
Infrared Spectrometry ◽  
Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

Reagents for detection of carboxylate anion ligands after chromatographic separation of zinc carboxylato complexes

2003 ◽  
Vol 16 (4) ◽  
pp. 286-288 ◽  
Author(s):  
Andrej Oriňák ◽  
Monika Ádámová ◽  
Ladislav Halás ◽  
Peter Tomčík ◽  
Martina Justinová
Keyword(s):  
Chromatographic Separation ◽  
Carboxylate Anion

Interpretation of the mechanisms of chromatographic separation on CN-silica. Part I: TLC of metal complexes

2002 ◽  
Vol 15 (5) ◽  
pp. 341-344 ◽  
Author(s):  
Helena Majstorović ◽  
Živoslav Tešić ◽  
Dušanka Milojković-Opsenica
Keyword(s):  
Metal Complexes ◽  
Chromatographic Separation

Interpretation of the mechanisms of chromatographic separation on CN-silica. Part II. TLC of some phenols

2004 ◽  
Vol 17 (1) ◽  
pp. 9-13 ◽  
Author(s):  
Helena Majstorović ◽  
Danijela Ratkov-Žebeljan ◽  
Živoslav Tešić ◽  
Dušanka Milojković-Opsenica
Keyword(s):  
Chromatographic Separation
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