Biomapping Salmonella on Broiler Carcasses in Poultry- Processing Plants: Case Studies

Foods ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 491
Author(s):  
Alejandra Ramirez-Hernandez ◽  
Ana K. Carrascal-Camacho ◽  
Andrea Varón-García ◽  
Mindy M. Brashears ◽  
Marcos X. Sanchez-Plata

The poultry industry in Colombia has implemented several changes and measures in chicken processing to improve sanitary operations and control pathogens’ prevalence. However, there is no official in-plant microbial profile reference data currently available throughout the processing value chains. Hence, this research aimed to study the microbial profiles and the antimicrobial resistance of Salmonella isolates in three plants. In total, 300 samples were collected in seven processing sites. Prevalence of Salmonella spp. and levels of Enterobacteriaceae were assessed. Additionally, whole-genome sequencing was conducted to characterize the isolated strains genotypically. Overall, the prevalence of Salmonella spp. in each establishment was 77%, 58% and 80% for plant A, B, and C. The mean levels of Enterobacteriaceae in the chicken rinsates were 5.03, 5.74, and 6.41 log CFU/mL for plant A, B, and C. Significant reductions were identified in the counts of post-chilling rinsate samples; however, increased levels were found in chicken parts. There were six distinct Salmonella spp. clusters with the predominant sequence types ST32 and ST28. The serotypes Infantis (54%) and Paratyphi B (25%) were the most commonly identified within the processing plants with a high abundance of antimicrobial resistance genes.


2009 ◽  
Vol 8 (10) ◽  
pp. 939-945 ◽  
Author(s):  
D.M. Paiva ◽  
M. Singh ◽  
K.S. Macklin ◽  
S.B. Price ◽  
J.B. Hess ◽  
...  

2008 ◽  
Vol 52 (No. 6) ◽  
pp. 262-266 ◽  
Author(s):  
E. Voslarova ◽  
B. Janackova B ◽  
F. Vitula ◽  
A. Kozak ◽  
V. Vecerek

Poor welfare is the cause of high mortality among hens and roosters transported to poultry processing plants. In the Czech Republic, death rates among hens and roosters in transport to poultry slaughter plants were monitored between 1997 and 2004, and their total mortality rate was in the 0.925% ± 0.479% range. Death rates among hens and roosters were influenced by the transport distance to poultry processing plants. The percentage of dead birds increased from 0.592% ± 0.575% at transport distances up to 50 km to 1.638% ± 0.952% at transport distances up to 300 km. The bird mortality was also influenced by the season of the year. Higher mortality rates were ascertained during the cold months of the year, specifically in October through to April.


1990 ◽  
Vol 22 (9) ◽  
pp. 17-24 ◽  
Author(s):  
O. Tünay ◽  
O. Tameroǧlu ◽  
N. Baykal ◽  
F. Afşarünal ◽  
E. Ödemiş

In this paper, the pretreatment approaches in the textile processing industry are discussed and two case studies which have been carried out in two textile processing plants are delineated. Case studies include the phases of process and pollution profiles, treatability study, conceptual design and evaluation of one-year actual treatment plant performances. One of the treatment schemes applied was chemical coagulation while the other was activated sludge. Therefore a comparison of two main types of pretreatment has been realised. Results indicate that biological treatment is more efficient and reliable in terms of organic matter removal as compared to chemical coagulation. Comparisons of cost of initial investment, cost and ease of operation, and sludge production are also made and discussed.


2011 ◽  
Vol 77 (16) ◽  
pp. 5722-5729 ◽  
Author(s):  
Karen T. Elvers ◽  
Victoria K. Morris ◽  
Diane G. Newell ◽  
Vivien M. Allen

ABSTRACTMany of the poultry flocks produced in the United Kingdom are colonized withCampylobacter, and the intensive nature of poultry processing usually results in contaminated carcasses. In this study, a previously reported molecular oligonucleotide probe method was used to track a specific flock-colonizing strain(s) on broiler carcasses during processing in two United Kingdom commercial poultry processing plants. FiveCampylobacter-positive flocks were sampled at four points along the processing line, postbleed, postpluck, prechill, and postchill, and twoCampylobacter-negative flocks processed immediately after positive flocks were sampled prechill.flaAwas sequenced fromCampylobacterstrains isolated from these flocks, and strain-specific probes were synthesized. Skin and cecal samples were plated onto selective agar to give individual colonies, which were transferred onto membranes. These were then hybridized with the strain- and genus-specific probes. For all the 5 positive flocks, there was a significant reduction in campylobacters postbleed compared to postpluck but no subsequent fall on sampling pre- and postchill, and the strain(s) predominating on the carcasses throughout processing came from the flock being processed. This indicates that strains from the abattoir environment were not a significant cause of carcass contamination in flocks with well-established campylobacter colonization. However, negative flocks that were preceded by positive flocks were contaminated by strains that did not generally originate from the predominating strains recovered from the ceca of the previous positive flocks. This suggests that the abattoir environment has a significant role in the contamination of carcasses from negative but not fully colonized flocks.


2014 ◽  
Vol 77 (3) ◽  
pp. 496-498 ◽  
Author(s):  
V. M. SOARES ◽  
J. G. PEREIRA ◽  
C. M. ZANETTE ◽  
L. A. NERO ◽  
J. P. A. N. PINTO ◽  
...  

Conveyor belts are widely used in food handling areas, especially in poultry processing plants. Because they are in direct contact with food and it is a requirement of the Brazilian health authority, conveyor belts are required to be continuously cleaned with hot water under pressure. The use of water in this procedure has been questioned based on the hypothesis that water may further disseminate microorganisms but not effectively reduce the organic material on the surface. Moreover, reducing the use of water in processing may contribute to a reduction in costs and emission of effluents. However, no consistent evidence in support of removing water during conveyor belt cleaning has been reported. Therefore, the objective of the present study was to compare the bacterial counts on conveyor belts that were or were not continuously cleaned with hot water under pressure. Superficial samples from conveyor belts (cleaned or not cleaned) were collected at three different times during operation (T1, after the preoperational cleaning [5 a.m.]; T2, after the first work shift [4 p.m.]; and T3, after the second work shift [1:30 a.m.]) in a poultry meat processing facility, and the samples were subjected to mesophilic and enterobacterial counts. For Enterobacteriaceae, no significant differences were observed between the conveyor belts, independent of the time of sampling or the cleaning process. No significant differences were observed between the counts of mesophilic bacteria at the distinct times of sampling on the conveyor belt that had not been subjected to continuous cleaning with water at 45°C. When comparing similar periods of sampling, no significant differences were observed between the mesophilic counts obtained from the conveyor belts that were or were not subjected to continuous cleaning with water at 45°C. Continuous cleaning with water did not significantly reduce microorganism counts, suggesting the possibility of discarding this procedure in chicken processing.


1970 ◽  
Vol 11 (3) ◽  
pp. 407-414 ◽  
Author(s):  
G. C. Mead ◽  
C. S. Impey

2017 ◽  
Vol 13 ◽  
pp. 176-182
Author(s):  
Anastasia I. Piskaeva ◽  
Yuriy Yu. Sidorin ◽  
Alexander Yu. Prosekov

The aim of this study was to examine and compare bactericidal properties of silver nanoparticles on strains isolated from poultry processing plants wastes. Strains Еscherichia coli В-6240, Salmonella typhimurium В3533 were used as a model test strains for Gram-negative bacteria and Lactobacillus sp. B3730, Lactobacillus salivarius B5001, Thermoactinomyces thalpophilus AC936 were used as a model test strains for Gram-positive bacteria.


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