scholarly journals Linking a rapid throughput plate-assay with high-sensitivity stable-isotope label LCMS quantification permits the identification and characterisation of low β-L-ODAP grass pea lines

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Peter M. F. Emmrich ◽  
Martin Rejzek ◽  
Lionel Hill ◽  
Paul Brett ◽  
Anne Edwards ◽  
...  
Keyword(s):  
Stable Isotope ◽  
Internal Standard ◽  
High Sensitivity ◽  
Spectrophotometric Assay ◽  
Rapid Screening ◽  
Grass Pea ◽  
Stable Isotope Label ◽  
Tissue Samples ◽  
Plate Assay ◽  
Accurate Quantification

Abstract Background Grass pea (Lathyrus sativus) is an underutilised crop with high tolerance to drought and flooding stress and potential for maintaining food and nutritional security in the face of climate change. The presence of the neurotoxin β-L-oxalyl-2,3-diaminopropionic acid (β-L-ODAP) in tissues of the plant has limited its adoption as a staple crop. To assist in the detection of material with very low neurotoxin toxin levels, we have developed two novel methods to assay ODAP. The first, a version of a widely used spectrophotometric assay, modified for increased throughput, permits rapid screening of large populations of germplasm for low toxin lines and the second is a novel, mass spectrometric procedure to detect very small quantities of ODAP for research purposes and characterisation of new varieties. Results A plate assay, based on an established spectrophotometric method enabling high-throughput ODAP measurements, is described. In addition, we describe a novel liquid chromatography mass spectrometry (LCMS)-based method for β-L-ODAP-quantification. This method utilises an internal standard (di-13C-labelled β-L-ODAP) allowing accurate quantification of β-L-ODAP in grass pea tissue samples. The synthesis of this standard is also described. The two methods are compared; the spectrophotometric assay lacked sensitivity and detected ODAP-like absorbance in chickpea and pea whereas the LCMS method did not detect any β-L-ODAP in these species. The LCMS method was also used to quantify β-L-ODAP accurately in different tissues of grass pea. Conclusions The plate-based spectrophotometric assay allows quantification of total ODAP in large numbers of samples, but its low sensitivity and inability to differentiate α- and β-L-ODAP limit its usefulness for accurate quantification in low-ODAP samples. Coupled to the use of a stable isotope internal standard with LCMS that allows accurate quantification of β-L-ODAP in grass pea samples with high sensitivity, these methods permit the identification and characterisation of grass pea lines with a very low ODAP content. The LCMS method is offered as a new ‘gold standard’ for β-L-ODAP quantification, especially for the validation of existing and novel low- and/or zero-β-L-ODAP genotypes.

EST-SSR analysis provides insights about genetic relatedness, population structure and gene flow in grass pea (Lathyrus sativus)

2015 ◽  
Vol 134 (3) ◽  
pp. 338-344 ◽  
Author(s):  
Khela R. Soren ◽  
Ashutosh Yadav ◽  
Gaurav Pandey ◽  
Priyanka Gangwar ◽  
Ashok K. Parihar ◽  
...  
Keyword(s):  
Population Structure ◽  
Gene Flow ◽  
Genetic Relatedness ◽  
Lathyrus Sativus ◽  
Grass Pea ◽  
Ssr Analysis

Neurolathyrism risk depends on type of grass pea preparation and on mixing with cereals and antioxidants

2005 ◽  
Vol 10 (2) ◽  
pp. 169-178 ◽  
Author(s):  
Haileyesus Getahun ◽  
Fernand Lambein ◽  
Michel Vanhoorne ◽  
Patrick Van der Stuyft
Keyword(s):  
Grass Pea

scholarly journals Effect of inoculation on the content of biogenic elements in the white lupine and grass pea

10.5219/1327 ◽  
2020 ◽  
Vol 14 ◽  
pp. 385-392
Author(s):  
Erika Zetochová ◽  
Alena Vollmannová ◽  
Ivana Tirdiľová
Keyword(s):  
Dry Matter ◽  
Lupinus Albus ◽  
Analytical Determination ◽  
Biogenic Elements ◽  
Grass Pea ◽  
Zn Content ◽  
White Lupine ◽  
Pea Seeds ◽  
Lathyrus Sativus L

The aim of this work was to determine the influence of the inoculant on the content of biogenic elements in tenoreign varieties of white lupine (Lupinus albus) and threearieties of grass pea (Lathyrus sativus L.) of Slovak origin. Rizobine was used as the inoculum before sowing. Dried and homogenised seed samples were mineralised using concentrated HNO3 using the MARS X ”“ Press 5 instrument. Analytical determination of macro- and microelements in all samples was performed using ARIAN DUO 240FS/240Z atomic absorption spectrometer. The determined values of biogenic elements content were expressed as mg.kg”“1 of dry matter. The average content of Cu was lower for both crops in variant A compared to variant B. The addition of the inoculant increased the content of Cu in both crops in lupine by 3.7% and grass pea by 10.94%. The Zn content of variant A in lupine was 19.14% higher than that of the grass pea. Grass pea seeds contained 97.76% less Mn than white lupine seeds in both variants. The Cr content of white lupine was 67.74% higher in variant A than in grass pea. The inoculant also increased the content of Cr in lupine by 25.0%. Lupine contained 30.02% less Fe in variant A and 41.27% less Fe in variant B than the grass pea. The results we have obtained show that Ca, K, and P are the predominant elements in the seeds of grass pea in both variants. By comparing selected types of legumes we found that the grass pea features a higher content of Cu, Fe, K, and P. The analysed seeds of white lupine had a higher content of Zn, Mn, Cr, Ni, Co, Na, Ca, and Mg. In conclusion, inoculation does not significantly affect the content of biogenic elements of selected legume species.

scholarly journals FORAGE YEILD AND COMPITITION INDICES OF CEREALS MIXED INTERCROPPING WITH FORAGE LEGUMES IN SULAIMANI REGION

2021 ◽  
Vol 52 (6) ◽  
pp. 1417-1430
Author(s):  
Ch. S. Jafar ◽  
Sh. I. Towfiq ◽  
J. Gh. Rafat
Keyword(s):  
Competitive Ratio ◽  
Dry Matter ◽  
Block Design ◽  
Winter Season ◽  
Forage Yield ◽  
Grass Pea ◽  
Forage Legumes ◽  
Booting Stage ◽  
Randomized Block Design ◽  
Competition Indices

The present study was conducted in Sulaimani region at two different locations, Kanipanka and Qlyasan during winter season of 2019-2020 to estimate the response of forage yield and some competition indices to the effect of crop pure stands and their mixtures of barley and triticale intercropped with narbon vetch and grass pea with some different patterns. The experiment was designed according to Completely Randomized Block Design with three replications. As the average of both location the maximum green forage yield was produced by pure narbon vetch 32.610 ton ha-1, while pure barley produce maximum dry forage yield and dry matter % reached 5.506 ton ha-1 and 8.55% at booting stage respectively, but the crop mixture barley/grass pea at a rate 2:1 produce maximum green and dry forage yield 32.083 and 5.616 ton ha-1 respectively at booting stage. The crop mixture barley/vetch 1:1 gave maximum dry matter% 17.88% at the same stage. The highest value for total LER was 1.401recorded by the mixture of triticale/grass pea at elongation stage, while the highest relative crowding coefficient was 1.285 recorded by the same mixture at a rate 1:1 at the same cutting stage. Maximum competitive ratio for cereals was 3.652 recorded by barley in the mixture barley/grass pea 1:2 at elongation stage, while for legume it was 2.292 for narbon vetch in the mixture triticale/vetch 2:1 at booting stage.

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