Resistance in Tomato and Other Lycopersicon Species to Insect and Mite Pests

2006 ◽  
pp. 487-519
Author(s):  
George Kennedy
Keyword(s):  
Lycopersicon Species

Salt tolerance in Lycopersicon species. II. Genetic effects and a search for associated traits

1993 ◽  
Vol 86 (6) ◽  
pp. 769-774 ◽  
Author(s):  
M. J. Asins ◽  
M. P. Bretó ◽  
E. A. Carbonell
Keyword(s):  
Salt Tolerance ◽  
Genetic Effects ◽  
Lycopersicon Species

Direct comparison of levels of genetic variation in tomato detected by a GACA-containing microsatellite probe and by random amplified polymorphic DNA

Genome ◽  
1994 ◽  
Vol 37 (3) ◽  
pp. 375-381 ◽  
Author(s):  
W. Rus-Kortekaas ◽  
M. J. M. Smulders ◽  
P. Arens ◽  
B. Vosman
Keyword(s):  
Tissue Culture ◽  
Genetic Variation ◽  
Somaclonal Variation ◽  
Random Amplified Polymorphic Dna ◽  
Dna Fingerprint ◽  
Pairwise Comparisons ◽  
Lycopersicon Pennellii ◽  
Lycopersicon Species ◽  
Polymorphic Bands ◽  
Simple Sequence

In this study, a direct comparison was made of the ability of four selected random amplified polymorphic DNA (RAPD) primers and a GACA-containing microsatellite probe to detect genetic variation in Lycopersicon. Of the 89 RAPD primers initially tested, 85 showed differences between a representative of Lycopersicon pennellii and L. esculentum, but only 4 distinguished among three L. esculentum cultivars. These four primers were subsequently tested on representatives of six Lycopersicon species. In pairwise comparisons of species, all or 14 of the 15 combinations could be distinguished by single primers. When the primers were tested on 15 L. esculentum cultivars, 90 of the 105 combinations could be distinguished by the four primers together. Finally, none of 118 tested primers showed reproducible differences among calli or progeny of régénérants from tissue culture, although some of the plants had inherited morphological mutations. The probe pWVA16, which detects GACA-containing microsatellites, could distinguish in TaqI-digested DNA the representatives of Lycopersicon species as well as all the L. esculentum cultivars tested. The probe was unable to detect polymorphisms among calli and the progeny of regenerants from tissue culture. An analysis of the results showed that the four selected RAPD primers were able to detect polymorphic bands among species at a frequency of 80%, and among cultivars at a frequency of 44%. In contrast, the microsatellite probe detected polymorphic bands at a frequency of 100 and 95%, respectively. The GACA-containing probe did not detect any common bands among the representatives of the six species, while band sharing with RAPDs was 48%. These results indicate that the two methods detect two types of DNA that differ in their degree of variability.Key words: DNA fingerprint, RAPD, simple sequence, somaclonal variation, tissue culture.

Genomic in situ hybridization analysis of a trigenomic hybrid involving Solanum and Lycopersicon species

Genome ◽  
2001 ◽  
Vol 44 (2) ◽  
pp. 299-304 ◽  
Author(s):  
S N Haider Ali ◽  
Dirk Jan Huigen ◽  
M S Ramanna ◽  
Evert Jacobsen ◽  
Richard GF Visser
Keyword(s):  
In Situ Hybridization ◽  
Chromosome Doubling ◽  
Genomic In Situ Hybridization ◽  
Potato Genome ◽  
Meiotic Chromosomes ◽  
Lycopersicon Species ◽  
Bridge Species ◽  
Genomic Probes ◽  
Homoeologous Chromosomes

A 4x potato (+) tomato fusion hybrid (2n = 4x = 48) was successfully backcrossed with a diploid Lycopersicon pennellii (2n = 2x = 24). Genomic in situ hybridization (GISH) on somatic and meiotic chromosomes confirmed that the progenies were triploids (2n = 3x = 36) and possessed three different genomes: potato, tomato, and L. pennellii. Therefore, they have been called trigenomic hybrids. Total genomic probes of both Lycopersicon species were found to hybridize mutually, whereas the potato genome was clearly differentiated. During metaphase I, bivalents were formed predominantly between tomato and L. pennellii chromosomes and the univalents of potato chromosomes were most common. Trivalents in all cases included homoeologous chromosomes of potato, tomato, and L. pennellii. However, the triploids were totally sterile as determined from extensive crossing. On chromosome doubling of triploids by shoot regeneration from callus, hexaploids (2n = 6x = 72) were obtained. Despite exhibiting clear allohexaploid behaviour by forming 36 bivalents at meiosis, these were also completely sterile like their triploid counterparts. In spite of this drawback, the prospects of chromosome pairing between potato L. pennellii and Solanum genomes does open the possibilities for bringing the two genera close.Key words: trigenomic triploids, GISH, bridge species, potato (+) tomato fusion hybrids.

Genetic differences in growth within and between Lycopersicon species

Euphytica ◽  
1991 ◽  
Vol 57 (3) ◽  
pp. 259-265
Author(s):  
Pim Lindhout ◽  
Gerard Pet ◽  
Ritsert Jansen ◽  
Hans Jansen
Keyword(s):  
Genetic Differences ◽  
Lycopersicon Species
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