Practical Advice to End the Practical Guide

2017 ◽  
Author(s):  
Linlin Zhang ◽  
Robert D. Reed

AbstractCRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.


2016 ◽  
Author(s):  
Krzysztof J. Gorgolewski ◽  
Russell A. Poldrack

AbstractRecent years have seen an increase in alarming signals regarding the lack of replicability in neuroscience, psychology, and other related fields. To avoid a widespread crisis in neuroimaging research and consequent loss of credibility in the public eye, we need to improve how we do science. This article aims to be a practical guide for researchers at any stage of their careers that will help them make their research more reproducible and transparent while minimizing the additional effort that this might require. The guide covers three major topics in open science (data, code, and publications) and offers practical advice as well as highlighting advantages of adopting more open research practices that go beyond improved transparency and reproducibility.


Author(s):  
Lilla Vicsek

Despite the fact that there are several practical advantages of online typed focus groups, this type of group questioning has not spread as widely as had been expected when it appeared as a new research option. One of the reasons for that might be that a major risk of these text-based focus groups is inadequate data quality. Unless certain measures are taken to prevent this, an analysis can face the problem of not being rich enough and not digging deep enough – which are often important criteria for good qualitative analysis. This article discusses how to deal with the problem and other possible pitfalls of this type of group discussion, and gives practical advice on how to obtain the best results from such discussions. It also gives suggestions which can be useful if a free chat platform is being used to conduct these groups. It argues that even nowadays with other online techniques available, online text-based focus groups can be useful – if executed properly.


2017 ◽  
Vol 71 (2) ◽  
Author(s):  
Reiko Tanimura ◽  
David Chart

Abstractwas a bestselling practical guide for women published in Japan in the late seventeenth century. This article translates volume three, concerning pregnancy and childbirth. It covers the whole period from first suspicion of childbirth to early childcare, and briefly describes childhood rituals. The advice can be divided into two broad categories: practical advice on diet, behaviour, and medications, and descriptions of customary rituals that should be performed on certain occasions. It also includes a significant amount of information on fortune telling for a child’s future, and a number of charms for such things as increasing the flow of milk. While it does include a list of the Buddhas who watch over the foetus, the contents are overwhelmingly practical and secular in character, with very little discussion of moral issues.


2002 ◽  
Vol 1 (2) ◽  
pp. 35-39
Author(s):  
Alison Evans ◽  
◽  
Martin Taylor ◽  

Diabetic ketoacidosis is a life-threatening medical emergency which requires prompt diagnosis and treatment. Understanding the mechanism by which lack of insulin causes dehydration and acidosis is helpful in managing this condition. Numerous guidelines exist to improve management but adherence to guidelines is often poor. This guide aims to provide practical advice for the doctors and nurses involved in the immediate management of these patients.


2007 ◽  
Vol 23 (3) ◽  
pp. 385-387 ◽  
Author(s):  
JOHN BROOME

It is not the job of philosophy to give direct practical advice either to people or to governments. Nevertheless, moral philosophy is immensely significant in practical matters. It influences the way we think and act, but only slowly as it filters through the process of public debate. I hope Weighing Lives will have a practical influence, but it is not meant to be a directly practical guide.


Author(s):  
Gerald Craver

In Successful Qualitative Research: A Practical Guide for Beginners (2013), Virginia Braun and Victoria Clarke present their method of thematic analysis as well as some strategies for successfully conducting qualitative research. The book is user-friendly and contains practical advice that may appeal to both novices and experienced researchers alike.


2019 ◽  
Vol 58 (2) ◽  
pp. 119
Author(s):  
Daniel G. Kipnis

The Chandos’ book series is designed to help the overworked librarian with practical advice using extensive case studies. The book begins with defining “informationist” and outlining the transformation from a traditional reference librarian to an informationist. The theme of the book deals with filling “information” gaps and each chapter focuses on different types of services that can help fill information gaps at a variety of health institutions.


2019 ◽  
Vol 73 (7) ◽  
pp. 418-422 ◽  
Author(s):  
Bethany Jill Williams ◽  
Darren Treanor

Numerous clinical pathology departments are deploying or planning to deploy digital pathology systems for all or part of their diagnostic output. Digital pathology is an evolving technology, and it is important that departments uphold or improve on current standards. Leeds Teaching Hospitals NHS Trust has been scanning 100% of histology slides since September 2018. In this practical paper, we will share our approach to training and validation, which has been incorporated into the Royal College of Pathologists’ guidance for digital pathology implementation. We will offer an overview of the Royal College endorsed training and validation protocol and the evidence base on which it is based. We will provide practical advice on implementation of the protocol and highlight areas of digital reporting that can prove difficult for the novice digital pathologist. In addition, we will share a detailed topographical list of types of diagnostic tasks and features which should form the basis of digital slide training sets.


2017 ◽  
Vol 103 (5) ◽  
pp. 267-273
Author(s):  
Lydia M C Green ◽  
Amanda J Friend ◽  
Rebecca J M Bardgett ◽  
Jonathan C Darling

The ability to interact with children and young people (CYP), appropriately examine and competently interpret signs is an essential skill for many medical practitioners and allied healthcare professionals; yet, how do we ensure competence in our students and trainees? One method is to include CYP in both formative and summative assessments; this provides an invaluable opportunity for examiners not only to evaluate the clinical interaction but also to gain an understanding of the CYP experience and what characteristics they value in a ‘good children’s doctor’. This paper explores the benefits and challenges of involving CYP in assessments and provides practical advice for course organisers, assessors and students when encountering CYP in assessment.


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