scholarly journals A practical guide to CRISPR/Cas9 genome editing in Lepidoptera

2017 ◽  
Author(s):  
Linlin Zhang ◽  
Robert D. Reed
Keyword(s):  
Experimental Design ◽  
Genome Editing ◽  
Model Systems ◽  
Strong Impact ◽  
Practical Advice ◽  
Entire Process ◽  
Practical Guide ◽  
Recent Advances ◽  
Detailed Protocol

AbstractCRISPR/Cas9 genome editing has revolutionized functional genetic work in many organisms and is having an especially strong impact in emerging model systems. Here we summarize recent advances in applying CRISPR/Cas9 methods in Lepidoptera, with a focus on providing practical advice on the entire process of genome editing from experimental design through to genotyping. We also describe successful targeted GFP knockins that we have achieved in butterflies. Finally, we provide a complete, detailed protocol for producing targeted long deletions in butterflies.

Recent advances of genome editing and related technologies in China

Gene Therapy ◽  
2020 ◽  
Vol 27 (7-8) ◽  
pp. 312-320
Author(s):  
Wen Sun ◽  
Haoyi Wang
Keyword(s):  
Genome Editing ◽  
Recent Advances

scholarly journals Efficient genome editing in multiple salmonid cell lines using ribonucleoprotein complexes

2020 ◽  
Author(s):  
Remi L. Gratacap ◽  
Ye Hwa Jin ◽  
Marina Mantsopoulou ◽  
Ross D. Houston
Keyword(s):  
Rainbow Trout ◽  
Atlantic Salmon ◽  
Infectious Diseases ◽  
Genome Editing ◽  
Cell Lines ◽  
Chinook Salmon ◽  
Model Systems ◽  
Fish Cell ◽  
Ribonucleoprotein Complexes ◽  
Salmonid Species

AbstractInfectious and parasitic diseases have major negative economic and animal welfare impacts on aquaculture of salmonid species. Improved knowledge of the functional basis of host response and genetic resistance to these diseases is key to developing preventative and treatment options. Cell lines provide a valuable model to study infectious diseases in salmonids, and genome editing using CRISPR provides an exciting avenue to evaluate the function of specific genes in those systems. While CRISPR/Cas9 has been successfully performed in a Chinook salmon cell line (CHSE-214), there are no reports to date of editing of cell lines derived from the most commercially relevant salmonid species Atlantic salmon and rainbow trout, which are difficult to transduce and therefore edit using lentivirus-mediated methods. In the current study, a method of genome editing of salmonid cell lines using ribonucleoprotein (RNP) complexes was optimised and tested in the most commonly-used salmonid fish cell lines; Atlantic salmon (SHK-1 and ASK cell lines), rainbow trout (RTG-2) and Chinook salmon (CHSE-214). Electroporation of RNP based on either Cas9 or Cas12a was efficient at targeted editing of all the tested lines (typically > 90 % cells edited), and the choice of enzyme expands the number of potential target sites for editing within the genomes of these species. These optimised protocols will facilitate functional genetic studies in salmonid cell lines, which are widely used as model systems for infectious diseases in aquaculture.

Recent Advances in Therapeutic Genome Editing in China

2018 ◽  
Vol 29 (2) ◽  
pp. 136-145 ◽  
Author(s):  
Yang Yang ◽  
Qingnan Wang ◽  
Qian Li ◽  
Ke Men ◽  
Zhiyao He ◽  
...  
Keyword(s):  
Genome Editing ◽  
Recent Advances

scholarly journals Genome editing in large animals: current status and future prospects

2019 ◽  
Vol 6 (3) ◽  
pp. 402-420 ◽  
Author(s):  
Jianguo Zhao ◽  
Liangxue Lai ◽  
Weizhi Ji ◽  
Qi Zhou
Keyword(s):  
Regenerative Medicine ◽  
Genome Editing ◽  
Biomedical Research ◽  
Human Disease ◽  
Gene Editing ◽  
Disease Modeling ◽  
Current Status ◽  
Future Prospects ◽  
Recent Advances ◽  
Large Animals

AbstractLarge animals (non-human primates, livestock and dogs) are playing important roles in biomedical research, and large livestock animals serve as important sources of meat and milk. The recently developed programmable DNA nucleases have revolutionized the generation of gene-modified large animals that are used for biological and biomedical research. In this review, we briefly introduce the recent advances in nuclease-meditated gene editing tools, and we outline these editing tools’ applications in human disease modeling, regenerative medicine and agriculture. Additionally, we provide perspectives regarding the challenges and prospects of the new genome editing technology.

scholarly journals Recent advances in structural studies of the CRISPR-Cas-mediated genome editing tools

2018 ◽  
Vol 6 (3) ◽  
pp. 438-451 ◽  
Author(s):  
Yuwei Zhu ◽  
Zhiwei Huang
Keyword(s):  
Nucleic Acids ◽  
Genome Editing ◽  
Adaptive Immunity ◽  
Structural Biology ◽  
Structural Studies ◽  
Guide Rnas ◽  
Recent Advances ◽  
Cas Proteins

Abstract Clustered regularly interspaced short palindromic repeats (CRISPR) and accompanying CRISPR-associated (Cas) proteins provide RNA-guided adaptive immunity for prokaryotes to defend themselves against viruses. The CRISPR-Cas systems have attracted much attention in recent years for their power in aiding the development of genome editing tools. Based on the composition of the CRISPR RNA-effector complex, the CRISPR-Cas systems can be divided into two classes and six types. In this review, we summarize recent advances in the structural biology of the CRISPR-Cas-mediated genome editing tools, which helps us to understand the mechanism of how the guide RNAs assemble with diverse Cas proteins to cleave target nucleic acids.

Cardiac muscle diseases in genetically engineered mice: evolution of molecular physiology

1995 ◽  
Vol 269 (3) ◽  
pp. H755-H766 ◽  
Author(s):  
K. R. Chien
Keyword(s):  
Cardiac Muscle ◽  
Large Body ◽  
Genetically Engineered ◽  
Mouse Genetics ◽  
Model Systems ◽  
Molecular Physiology ◽  
Muscle Diseases ◽  
Recent Advances ◽  
Genetically Engineered Mice

Recent advances in molecular, cellular, and genetically based technologies now offer the possibility of generating genetically engineered mice that display physiological phenotypes with direct relevance to human pathophysiological states. The ability to create gene ablations, gene duplications, and gene modifications should allow the use of genetic approaches to map in vivo pathways responsible for complex physiological phenotypes. Recent work from our laboratory utilizing this approach to study cardiac muscle diseases in both the adult context (cardiac hypertrophy) and in the embryonic context (congenital ventricular defects) will be discussed, as well as the steps that led to the generation and characterization of these novel mouse model systems. A large body of work from independent laboratories now points to the inception of a new field of molecular physiology that will fuse mouse genetics and in vivo physiology using appropriate miniaturized physiological technology. Recent advances and prospects for future directions are summarized.

scholarly journals The Hitchhiker's guide to biocatalysis: recent advances in the use of enzymes in organic synthesis

2020 ◽  
Vol 11 (10) ◽  
pp. 2587-2605 ◽  
Author(s):  
Roger A. Sheldon ◽  
Dean Brady ◽  
Moira L. Bode
Keyword(s):  
Organic Synthesis ◽  
Practical Guide ◽  
Recent Advances ◽  
Enzymes In Organic Synthesis

Enzymes are excellent catalysts that are increasingly being used in industry and academia. This Perspective provides a general and practical guide to enzymes and their synthetic potential, primarily aimed at synthetic organic chemists.

Recent Advances in NMR Diffusion Techniques for Studying Drug Binding

2003 ◽  
Vol 56 (9) ◽  
pp. 855 ◽  
Author(s):  
William S. Price
Keyword(s):  
Drug Development ◽  
Study Drug ◽  
Drug Binding ◽  
Model Systems ◽  
Aqueous Samples ◽  
Recent Advances ◽  
Physiological Relevance ◽  
Nmr Diffusion

Characterizing the binding of ligands to macromolecular receptors in solution is important to many areas of chemistry, biology, and nanobiotechnology, but perhaps most notably to drug development. NMR has proven to be particularly useful for such studies, but the systems studied have generally been restricted to model systems with dubious physiological relevance. This paper reviews the use of NMR diffusion measurements to study drug binding and two recent advances that enable measurements to be conducted in more sensitive higher-field NMR spectrometers in non-deuterated aqueous samples.

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