Regulation of Metabolite Synthesis in Plants

2006 ◽  
pp. 101-141
Author(s):  
Peter Kaufman ◽  
Leland Cseke
Keyword(s):  
Metabolite Synthesis

Engineering and application of P450 monooxygenases in pharmaceutical and metabolite synthesis

2013 ◽  
Vol 17 (2) ◽  
pp. 271-275 ◽  
Author(s):  
Jill M Caswell ◽  
Maeve O’Neill ◽  
Steve JC Taylor ◽  
Thomas S Moody
Keyword(s):  
P450 Monooxygenases ◽  
Metabolite Synthesis

scholarly journals Effects of Short-Term Exposure to Low Temperatures on Proline, Pigments, and Phytochemicals Level in Kale (Brassica oleracea var. acephala)

Horticulturae ◽  
2021 ◽  
Vol 7 (10) ◽  
pp. 341
Author(s):  
Valentina Ljubej ◽  
Erna Karalija ◽  
Branka Salopek-Sondi ◽  
Dunja Šamec
Keyword(s):  
Brassica Oleracea ◽  
Phenolic Acids ◽  
Low Temperatures ◽  
Freezing Temperature ◽  
Total Phenolic ◽  
Total Polyphenol ◽  
Good Tolerance ◽  
Short Term Exposure ◽  
Health Related ◽  
Metabolite Synthesis

Kale (Brassica oleracea var acephala) is known as a vegetable with good tolerance of environmental stress and numerous beneficial properties for human health, which are attributed to different phytochemicals. In the present study, investigation of how low temperatures affect proline, pigments and specialized metabolites content was performed using 8-weeks old kale plants subjected to chilling (at 8 °C, for 24 h) followed by short freezing (at −8 °C, for 1 h after previous acclimation at 8 °C, for 23 h). Plants growing at 21 °C served as a control. In both groups of plants (exposed to low temperatures and exposed to short freezing) a significant increase in proline content (14% and 49%, respectively) was recorded. Low temperatures (8 °C) induced an increase of pigments (total chlorophylls 7%) and phytochemicals (phenolic acids 3%; flavonoids 5%; carotenoids 15%; glucosinolates 21%) content, while exposure to freezing showed a different trend dependent upon observed parameter. After freezing, the content of chlorophylls, carotenoids, and total phenolic acids retained similar levels as in control plants and amounted to 14.65 ± 0.36 mg dw g−1, 2.58 ± 0.05 mg dw g−1 and 13.75 ± 0.07 mg dw CEA g−1, respectively. At the freezing temperature, total polyphenol content increased 13% and total flavonoids and glucosinolates content decreased 21% and 54%, respectively. Our results suggest that acclimatization (23 h at 8 °C) of kale plants can be beneficial for the accumulation of pigments and phytochemicals, while freezing temperatures affect differently specialized metabolite synthesis. The study suggests that growing temperature during kale cultivation must be considered as an important parameter for producers that are orientated towards production of crops with an increasing content of health-related compounds.

scholarly journals Lilium Regale Wilson WRKY3 Modulates an Antimicrobial Peptide Gene, Lrdef1, During Response to Fusarium Oxysporum

2021 ◽  
Author(s):  
Zie Wang ◽  
Jie Deng ◽  
Tingting Liang ◽  
Linlin Su ◽  
Lilei Zheng ◽  
...  
Keyword(s):  
Antimicrobial Peptide ◽  
Fusarium Oxysporum ◽  
Transgenic Tobacco ◽  
Sequencing Analysis ◽  
Lilium Regale ◽  
Expression Activity ◽  
Metabolite Synthesis ◽  
Lilium Regale Wilson ◽  
Peptide Gene ◽  
Higher Sensitivity

Abstract Background: WRKY transcription factors (TFs) play vital roles in plant growth and development, secondary metabolite synthesis, and response to biotic and abiotic stresses. In a previous transcriptome sequencing analysis of Lilium regale Wilson, we identified multiple WRKY TFs that respond to exogenous methyl jasmonate treatment and lily Fusarium wilt (Fusarium oxysporum).Results: In the present study, the WRKY TF LrWRKY3 was further analyzed to reveal its function in defense response to F. oxysporum. The LrWRKY3 protein was localized in the plant cell nucleus, and LrWRKY3 transgenic tobacco lines showed higher resistance to F. oxysporum compared with wild-type (WT) tobacco. In addition, some genes related to jasmonic acid (JA) biosynthesis, salicylic acid (SA) signal transduction, and disease resistance had higher transcriptional levels in the LrWRKY3 transgenic tobacco lines than in the WT. On the contrary, L. regale scales transiently expressing LrWRKY3 RNA interference fragments showed higher sensitivity to F. oxysporum infection. Moreover, a F. oxysporum-induced defensin gene, Def1, was isolated from L. regale, and the recombinant protein LrDef1 isolated and purified from Escherichia coli possessed antifungal activity to several phytopathogens, including F. oxysporum. Furthermore, co-expression of LrWRKY3 and the LrDef1 promoter in tobacco evidently up-regulated the expression activity of the LrDef1 promoter.Conclusions: These results clearly indicate that LrWRKY3 is an important positive regulator in response to F. oxysporum infection, and one of its targets is the antimicrobial peptide gene LrDef1.

Kinetic model of cell growth and secondary metabolite synthesis in plant cell culture ofThalictrum rugosum

1999 ◽  
Vol 4 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Jeong-Woo Choi ◽  
Young-Kee Kim ◽  
Won Hong Lee ◽  
Henrik Pedersen ◽  
Chee-Kok Chin
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Kinetic Model ◽  
Secondary Metabolite ◽  
Plant Cell ◽  
Plant Cell Culture ◽  
Metabolite Synthesis

scholarly journals De novo transcriptome provides insights into the growth behaviour and resveratrol and trans-stilbenes biosynthesis in Dactylorhiza hatagirea - An endangered alpine terrestrial orchid of western Himalaya

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nisha Dhiman ◽  
Nitesh Kumar Sharma ◽  
Pooja Thapa ◽  
Isha Sharma ◽  
Mohit Kumar Swarnkar ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
De Novo ◽  
Natural Habitat ◽  
Sugar Metabolism ◽  
Western Himalaya ◽  
Metabolite Profile ◽  
Clonal Plants ◽  
Phenylpropanoid Pathway ◽  
Terrestrial Orchid ◽  
Metabolite Synthesis

Abstract This is the first report on de novo transcriptome of Dactylorhiza hatagirea, a critically-endangered, terrestrial orchid of alpine Himalayas. The plant is acclaimed for medicinal properties but little is known about its secondary-metabolites profile or cues regulating their biosynthesis. De novo transcriptome analysis was therefore, undertaken to gain basic understanding on these aspects, while circumventing the acute limitation of plant material availability. 65,384 transcripts and finally, 37,371 unigenes were assembled de novo from a total of 236 million reads obtained from shoot, tuber and leaves of the plant. Dominance of differentially-expressing-genes (DEGs) related to cold-stress-response and plant-hormone-signal-transduction; and those involved in photosynthesis, sugar-metabolism and secondary-metabolite-synthesis provided insights into carbohydrate-partitioning in the plant during its preparation for freezing winter at natural habitat. DEGs of glucomannan, ascorbic acid, carotenoids, phylloquinone/naphthoquinones, indole alkaloids, resveratrol and stilbene biosynthesis revealed the secondary-metabolite profile of D. hatagirea. UHPLC results confirmed appreciable amounts of resveratrol and trans-stilbene in D. hatagirea tubers, for the first time. Expression analysis of 15 selected genes including those of phenylpropanoid pathway confirmed the validity of RNA-seq data. Opportunistic growth, temperature- and tissue-specific-differential-expression of secondary metabolite biosynthesis and stress tolerant genes were confirmed using clonal plants growing at 8, 15 and 25 °C.

scholarly journals Metabolome and Transcriptome Analysis of Hexaploid Solidago canadensis Roots Reveals its Invasive Capacity Related to Polyploidy

Genes ◽  
2020 ◽  
Vol 11 (2) ◽  
pp. 187 ◽  
Author(s):  
Miao Wu ◽  
Yimeng Ge ◽  
Chanchan Xu ◽  
Jianbo Wang
Keyword(s):  
Gene Expression ◽  
Transcriptome Analysis ◽  
Gene Expression Regulation ◽  
Mapk Signaling ◽  
Vital Role ◽  
Solidago Canadensis ◽  
Metabolome Analysis ◽  
Regulation Of Expression ◽  
Invasive Capacity ◽  
Metabolite Synthesis

Polyploid plants are more often invasive species than their diploid counterparts. As the invasiveness of a species is often linked to its production of allelopathic compounds, we hypothesize that differences in invasive ability between cytotypes may be due to their different ability to synthesize allelopathic metabolites. We test this using two cytotypes of Solidago canadensis as the model and use integrated metabolome and transcriptome data to resolve the question. Metabolome analysis identified 122 metabolites about flavonoids, phenylpropanoids and terpenoids, of which 57 were differentially accumulated between the two cytotypes. Transcriptome analysis showed that many differentially expressed genes (DEGs) were enriched in ‘biosynthesis of secondary metabolites’, ‘plant hormone signal transduction’, and ‘MAPK signaling’, covering most steps of plant allelopathic metabolite synthesis. Importantly, the differentially accumulated flavonoids, phenylpropanoids and terpenoids were closely correlated with related DEGs. Furthermore, 30 miRNAs were found to be negatively associated with putative targets, and they were thought to be involved in target gene expression regulation. These miRNAs probably play a vital role in the regulation of metabolite synthesis in hexaploid S. canadensis. The two cytotypes of S. canadensis differ in the allelopathic metabolite synthesis and this difference is associated with regulation of expression of a range of genes. These results suggest that changes in gene expression may underlying the increased invasive potential of the polyploidy.

Effect of Light on Growth and Metabolite Synthesis inCandida Albicans

Mycologia ◽  
1979 ◽  
Vol 71 (4) ◽  
pp. 773-785 ◽  
Author(s):  
Cora G. Saltarelli ◽  
C. Paveljack Coppola
Keyword(s):  
Metabolite Synthesis ◽  
Effect Of Light ◽  
Incandida Albicans
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