Cell cultures of the wild sunflower Helianthus maximiliani schrader: growth and secondary metabolite synthesis

1988 ◽  
Vol 7 (3) ◽  
pp. 197-199 ◽  
Author(s):  
Ingeborg Roewer ◽  
Tom J. Mabry
Keyword(s):  
Secondary Metabolite ◽  
Cell Cultures ◽  
Wild Sunflower ◽  
Metabolite Synthesis

Ethephon Enhancement of Secondary Metabolite Synthesis in Plant Cell Cultures

1988 ◽  
Vol 4 (3) ◽  
pp. 184-188 ◽  
Author(s):  
G. H. Cho ◽  
D. I. Kim ◽  
H. Pedersen ◽  
C-K. Chin
Keyword(s):  
Secondary Metabolite ◽  
Plant Cell ◽  
Cell Cultures ◽  
Plant Cell Cultures ◽  
Metabolite Synthesis

Kinetic model of cell growth and secondary metabolite synthesis in plant cell culture ofThalictrum rugosum

1999 ◽  
Vol 4 (2) ◽  
pp. 129-137 ◽  
Author(s):  
Jeong-Woo Choi ◽  
Young-Kee Kim ◽  
Won Hong Lee ◽  
Henrik Pedersen ◽  
Chee-Kok Chin
Keyword(s):  
Cell Culture ◽  
Cell Growth ◽  
Kinetic Model ◽  
Secondary Metabolite ◽  
Plant Cell ◽  
Plant Cell Culture ◽  
Metabolite Synthesis

scholarly journals De novo transcriptome provides insights into the growth behaviour and resveratrol and trans-stilbenes biosynthesis in Dactylorhiza hatagirea - An endangered alpine terrestrial orchid of western Himalaya

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Nisha Dhiman ◽  
Nitesh Kumar Sharma ◽  
Pooja Thapa ◽  
Isha Sharma ◽  
Mohit Kumar Swarnkar ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
De Novo ◽  
Natural Habitat ◽  
Sugar Metabolism ◽  
Western Himalaya ◽  
Metabolite Profile ◽  
Clonal Plants ◽  
Phenylpropanoid Pathway ◽  
Terrestrial Orchid ◽  
Metabolite Synthesis

Abstract This is the first report on de novo transcriptome of Dactylorhiza hatagirea, a critically-endangered, terrestrial orchid of alpine Himalayas. The plant is acclaimed for medicinal properties but little is known about its secondary-metabolites profile or cues regulating their biosynthesis. De novo transcriptome analysis was therefore, undertaken to gain basic understanding on these aspects, while circumventing the acute limitation of plant material availability. 65,384 transcripts and finally, 37,371 unigenes were assembled de novo from a total of 236 million reads obtained from shoot, tuber and leaves of the plant. Dominance of differentially-expressing-genes (DEGs) related to cold-stress-response and plant-hormone-signal-transduction; and those involved in photosynthesis, sugar-metabolism and secondary-metabolite-synthesis provided insights into carbohydrate-partitioning in the plant during its preparation for freezing winter at natural habitat. DEGs of glucomannan, ascorbic acid, carotenoids, phylloquinone/naphthoquinones, indole alkaloids, resveratrol and stilbene biosynthesis revealed the secondary-metabolite profile of D. hatagirea. UHPLC results confirmed appreciable amounts of resveratrol and trans-stilbene in D. hatagirea tubers, for the first time. Expression analysis of 15 selected genes including those of phenylpropanoid pathway confirmed the validity of RNA-seq data. Opportunistic growth, temperature- and tissue-specific-differential-expression of secondary metabolite biosynthesis and stress tolerant genes were confirmed using clonal plants growing at 8, 15 and 25 °C.

Comparative iTRAQ Proteomic Analysis Provides the Molecular Basis of the Metabolites in Cultivated Glycyrrhiza uralensis and its Wild-Type

2020 ◽  
Vol 17 (5) ◽  
pp. 365-378
Author(s):  
Chengcheng Wang ◽  
Lihong Chen ◽  
Zhichen Cai ◽  
Sijing Feng ◽  
Moyi Yue ◽  
...  
Keyword(s):  
Secondary Metabolite ◽  
Molecular Mechanisms ◽  
Glycyrrhiza Uralensis ◽  
Differentially Expressed ◽  
Primary Metabolism ◽  
Differentially Expressed Proteins ◽  
Metabolite Formation ◽  
Glycyrrhiza Uralensis Fisch ◽  
Significant Difference ◽  
Metabolite Synthesis

Background: Licorice is an herbal medicine applied extensively worldwide, and most of the licorice for clinical consumption is provided by Glycyrrhiza uralensis Fisch. Evidence suggests that there is a significant difference in the metabolite composition of licorice from different ecotypes. Objective: To better understand the proteomic changes and molecular mechanisms of metabolite formation in wild and cultivated Glycyrrhiza uralensis Fisch. Methods: Firstly, we established a proteome database by annotating protein sequences according to the genomic and transcriptomic data of G. uralensis. Then, iTRAQ and LC-MS/MS were applied to detect significant protein changes between cultivated and wild G. uralensis. A total of 2751 validated proteins were obtained with high confidence, and 333 were differentially expressed. Differentially expressed proteins were identified and analysed by GO, KEGG, and STRING for network and pathway enrichment. Ultimately, we combined the iTRAQ results with our previous investigation on metabolites to understand the molecular mechanisms underlying metabolite accumulation. Results: The results showed that differentially expressed proteins were mainly involved in the anabolism of carbohydrates and important amino acids that participate in primary metabolism and secondary metabolite synthesis. Another important pathway is the synthesis of flavonoids, which are generally accepted as important bioactive constituents of G. uralensis, and the accumulation of flavonoids in different synthesis stages in two ecotypes of G. uralensis was diverse. Therefore, the differentially abundant proteins in wild and cultivated G. uralensis possibly resulted in differences in medicinal compounds. Conclusion: Our study will provide novel clues for revealing the molecular mechanism of secondary metabolite synthesis as well as quality formation in wild and cultivated G. uralensis.

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