Multiplexed Integrated Online Sample Preparation for DNA Sequencing and Genetic Typing

2002 ◽  
Author(s):  
Edward Yeung
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
Genetic Typing

scholarly journals Semi-Automated Library Preparation for High-Throughput DNA Sequencing Platforms

2010 ◽  
Vol 2010 ◽  
pp. 1-8 ◽  
Author(s):  
Eveline Farias-Hesson ◽  
Jonathan Erikson ◽  
Alexander Atkins ◽  
Peidong Shen ◽  
Ronald W. Davis ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
High Throughput ◽  
Magnetic Beads ◽  
Dna Barcode ◽  
Cost Effective ◽  
Dna Libraries ◽  
Emulsion Pcr ◽  
High Throughput Dna Sequencing ◽  
Sequencing Platforms

Next-generation sequencing platforms are powerful technologies, providing gigabases of genetic information in a single run. An important prerequisite for high-throughput DNA sequencing is the development of robust and cost-effective preprocessing protocols for DNA sample library construction. Here we report the development of a semi-automated sample preparation protocol to produce adaptor-ligated fragment libraries. Using a liquid-handling robot in conjunction with Carboxy Terminated Magnetic Beads, we labeled each library sample using a unique 6 bp DNA barcode, which allowed multiplex sample processing and sequencing of 32 libraries in a single run using Applied Biosystems' SOLiD sequencer. We applied our semi-automated pipeline to targeted medical resequencing of nuclear candidate genes in individuals affected by mitochondrial disorders. This novel method is capable of preparing as much as 32 DNA libraries in 2.01 days (8-hour workday) for emulsion PCR/high throughput DNA sequencing, increasing sample preparation production by 8-fold.

Automated Digital Microfluidic Sample Preparation for Next-Generation DNA Sequencing

2011 ◽  
Vol 16 (6) ◽  
pp. 405-414 ◽  
Author(s):  
Hanyoup Kim ◽  
Michael S. Bartsch ◽  
Ronald F. Renzi ◽  
Jim He ◽  
James L. Van de Vreugde ◽  
...  
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
Next Generation ◽  
Next Generation Dna Sequencing ◽  
Digital Microfluidic

scholarly journals An Automated Sample Preparation System for Large-Scale DNA Sequencing

1999 ◽  
Vol 9 (5) ◽  
pp. 457-462 ◽  
Author(s):  
Andre Marziali ◽  
Thomas D. Willis ◽  
Nancy A. Federspiel ◽  
Ronald W. Davis
Keyword(s):  
Sample Preparation ◽  
Dna Sequencing ◽  
High Throughput ◽  
Large Scale ◽  
Low Cost ◽  
High Quality ◽  
Dna Templates ◽  
Sequencing Technologies ◽  
Front End ◽  
Automated Sample Preparation

Recent advances in DNA sequencing technologies, both in the form of high lane-density gels and automated capillary systems, will lead to an increased requirement for sample preparation systems that operate at low cost and high throughput. As part of the development of a fully automated sequencing system, we have developed an automated subsystem capable of producing 10,000 sequence-ready ssDNA templates per day from libraries of M13 plaques at a cost of $0.29 per sample. This Front End has been in high throughput operation since June, 1997 and has produced > 400,000 high-quality DNA templates.

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