Hypoxia Differently Modulates Gene Expression of Inositol 1,4,5-Trisphosphate Receptors in Mouse Kidney and HEK 293 Cell Line

2008 ◽  
Vol 1148 (1) ◽  
pp. 421-427 ◽  
Author(s):  
Dana Jurkovicova ◽  
Barbora Sedlakova ◽  
Lubica Lacinova ◽  
Juraj Kopacek ◽  
Zdena Sulova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Mouse Kidney ◽  
Hek 293 ◽  
Inositol 1,4,5 Trisphosphate ◽  
Hek 293 Cell Line

scholarly journals Transfection optimisation in HEK-293 cell line

2015 ◽  
Vol 1 (1) ◽  
pp. 121-129
Author(s):  
Jay Prakash Sah ◽  
M Salahuddin ◽  
M Abu Sayed ◽  
Abu Saim Al Salauddin ◽  
Md Sayfullah ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Cell Line ◽  
Plasmid Dna ◽  
Time Frame ◽  
Cell Number ◽  
Optimum Conditions ◽  
Hek 293 ◽  
Maximum Expression ◽  
Hek 293 Cell Line

Transfection is the most popular experiments among biological scientist to study the gene expression. But it is really tedious to find out the optimum conditions for protein expression for transfection while working with plasmid DNA. In our present study, the main objective was to setup suitable conditions to study the protein expression. From different conditions, protein expression was observed for 2?g of DNA. We tried different cell number and time frame and got 6.25×105cell/ well of 6 well and 24 hour incubation were the best condition for maximum expression of protein. In our experiment 1:3 ratio of DNA: Lipfectamine was the best for optimum expression of protein in mammalian cell line.Asian J. Med. Biol. Res. June 2015, 1(1): 121-129

scholarly journals Chemically mimicked hypoxia modulates gene expression and protein levels of the sodium calcium exchanger in HEK 293 cell line via HIF-1α

2011 ◽  
Vol 30 (Special Issue) ◽  
pp. 196-206 ◽  
Author(s):  
S. Hudecova ◽  
L. Lencesova ◽  
L. Csaderova ◽  
M. Sirova ◽  
D. Cholujova ◽  
...  
Keyword(s):  
Gene Expression ◽  
Cell Line ◽  
Sodium Calcium Exchanger ◽  
Hek 293 ◽  
Protein Levels ◽  
Sodium Calcium ◽  
Hek 293 Cell Line

The time course of Akt and ERK activation on XIAP expression in HEK 293 cell line

2009 ◽  
Vol 37 (4) ◽  
pp. 2037-2042 ◽  
Author(s):  
Mousa Abkhezr ◽  
Ali Reza Keramati ◽  
Seyed Nasser Ostad ◽  
Jamshid Davoodi ◽  
Mohammad H. Ghahremani
Keyword(s):  
Cell Line ◽  
Time Course ◽  
Hek 293 ◽  
Erk Activation ◽  
Xiap Expression ◽  
Hek 293 Cell Line

scholarly journals Enhancing the Butyrylcholinesterase Activity in HEK-293 Cell Line by Dual-Promoter Vector Decorated on Lipofectamine

2020 ◽  
Vol Volume 14 ◽  
pp. 3589-3599
Author(s):  
Vida Mirzaie ◽  
Touba Eslaminejad ◽  
Homayoon Babaei ◽  
Seyed Noureddin Nematollahi-Mahani
Keyword(s):  
Cell Line ◽  
Hek 293 ◽  
Dual Promoter ◽  
Butyrylcholinesterase Activity ◽  
Hek 293 Cell Line

scholarly journals Design, synthesis and biological evaluation of 1,2,3-triazole based 2-aminobenzimidazoles as novel inhibitors of LasR dependent quorum sensing in Pseudomonas aeruginosa

RSC Advances ◽  
2019 ◽  
Vol 9 (50) ◽  
pp. 29273-29292 ◽  
Author(s):  
Singireddi Srinivasarao ◽  
Adinarayana Nandikolla ◽  
Shashidhar Nizalapur ◽  
Tsz Tin Yu ◽  
Sravani Pulya ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Quorum Sensing ◽  
Cell Line ◽  
Biological Evaluation ◽  
Design Synthesis ◽  
Hek 293 ◽  
Line P ◽  
Synthesis And Biological Evaluation ◽  
Hek 293 Cell Line

Out of 40 benzimdazoles, 12 exhibited potent QSI activity against P. aeruginosa6p, most active QSI is docked to LasR and is less toxic against HEK 293 cell line.

Development of a cell-based assay for screening of phosphodiesterase 10A (PDE10A) inhibitors using a stable recombinant HEK-293 cell line expressing high levels of PDE10A

2008 ◽  
Vol 49 (2) ◽  
pp. 129 ◽  
Author(s):  
Roop Singh Bora ◽  
Dikshi Gupta ◽  
Renu Malik ◽  
Sonia Chachra ◽  
Pratibha Sharma ◽  
...  
Keyword(s):  
Cell Line ◽  
Hek 293 ◽  
A Cell ◽  
Phosphodiesterase 10A ◽  
Hek 293 Cell Line

Changes in ultrastructure and endogenous ionic channels activity during culture of HEK 293 cell line

2007 ◽  
Vol 567 (1-2) ◽  
pp. 10-18 ◽  
Author(s):  
Martina Kurejová ◽  
Branislav Uhrík ◽  
Zdenka Sulová ◽  
Barbora Sedláková ◽  
Olga Križanová ◽  
...  
Keyword(s):  
Cell Line ◽  
Ionic Channels ◽  
Hek 293 ◽  
Hek 293 Cell Line
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