scholarly journals The relationship between non-HDL cholesterol and macrophage phenotypes in human adipose tissue

2016 ◽  
Vol 57 (10) ◽  
pp. 1899-1905 ◽  
Author(s):  
Rudolf Poledne ◽  
Ivana Kralova Lesna ◽  
Anna Kralova ◽  
Jiri Fronek ◽  
Sona Cejkova
Keyword(s):  
Adipose Tissue ◽  
Hdl Cholesterol ◽  
Human Adipose Tissue ◽  
Macrophage Phenotypes ◽  
The Relationship

Importance of the Cyclic AMP Concentration for the Rate of Lipolysis in Human Adipose Tissue

1980 ◽  
Vol 59 (3) ◽  
pp. 199-201 ◽  
Author(s):  
P. Arner ◽  
J. Östman
Keyword(s):  
Adipose Tissue ◽  
Cyclic Amp ◽  
Subcutaneous Adipose Tissue ◽  
Human Adipose Tissue ◽  
Strong Positive Correlation ◽  
Glycerol Release ◽  
Two Parameters ◽  
Subcutaneous Adipose ◽  
The Relationship ◽  
Release Of Glycerol

1. The activation of lipolysis on incubation of human subcutaneous adipose tissue was examined in terms of the relationship between the release of glycerol and the concentration of tissue cyclic AMP. 2. A strong positive correlation was obtained between the maximum concentration of cyclic AMP and the rate of glycerol release in the presence of noradrenaline (r = 0.9), whereas, in the basal state, these two parameters were only weakly correlated (r = 0.45). 3. It appears that the noradrenaline-induced rate of lipolysis depends upon the maximal concentration of cyclic AMP that is present in human adipose tissue.

scholarly journals Integrative mRNA-microRNA analyses reveal novel interactions related to insulin sensitivity in human adipose tissue

2016 ◽  
Vol 48 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Tyler J. Kirby ◽  
R. Grace Walton ◽  
Brian Finlin ◽  
Beibei Zhu ◽  
Resat Unal ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Insulin Sensitivity ◽  
Human Adipose Tissue ◽  
Whole Body ◽  
Insulin Resistant ◽  
Novel Interactions ◽  
Subcutaneous Adipose ◽  
The Relationship ◽  
Microrna Microarray

Adipose tissue has profound effects on whole-body insulin sensitivity. However, the underlying biological processes are quite complex and likely multifactorial. For instance, the adipose transcriptome is posttranscriptionally modulated by microRNAs, but the relationship between microRNAs and insulin sensitivity in humans remains to be determined. To this end, we utilized an integrative mRNA-microRNA microarray approach to identify putative molecular interactions that regulate the transcriptome in subcutaneous adipose tissue of insulin-sensitive (IS) and insulin-resistant (IR) individuals. Using the NanoString nCounter Human v1 microRNA Expression Assay, we show that 17 microRNAs are differentially expressed in IR vs. IS. Of these, 16 microRNAs (94%) are downregulated in IR vs. IS, including miR-26b, miR-30b, and miR-145. Using Agilent Human Whole Genome arrays, we identified genes that were predicted targets of miR-26b, miR-30b, and miR-145 and were upregulated in IR subjects. This analysis produced ADAM22, MYO5A, LOX, and GM2A as predicted gene targets of these microRNAs. We then validated that miR-145 and miR-30b regulate these mRNAs in differentiated human adipose stem cells. We suggest that use of bioinformatic integration of mRNA and microRNA arrays yields verifiable mRNA-microRNA pairs that are associated with insulin resistance and can be validated in vitro.

scholarly journals The Relationship of a Combination of Human Adipose Tissue-Derived Stem Cells and Frozen Fat with the Survival Rate of Transplanted Fat

2015 ◽  
Vol 42 (6) ◽  
pp. 677 ◽  
Author(s):  
Ki-Young Ha ◽  
Hojin Park ◽  
Seung-Ha Park ◽  
Byung-Il Lee ◽  
Yi-Hwa Ji ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Survival Rate ◽  
Human Adipose Tissue ◽  
Relationship Of ◽  
The Relationship

scholarly journals Monocyte Chemoattractant Protein-1 Release Is Higher in Visceral than Subcutaneous Human Adipose Tissue (AT): Implication of Macrophages Resident in the AT

2005 ◽  
Vol 90 (4) ◽  
pp. 2282-2289 ◽  
Author(s):  
Jens M. Bruun ◽  
Aina S. Lihn ◽  
Steen B. Pedersen ◽  
Bjørn Richelsen
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
Low Grade ◽  
Mrna Levels ◽  
Vascular Cells ◽  
Monocyte Chemoattractant Protein 1 ◽  
Monocyte Chemoattractant ◽  
Monocyte Chemoattractant Protein ◽  
Inflammatory State ◽  
The Relationship

Abstract Human adipose tissue (AT) produces several adipokines including monocyte chemoattractant protein (MCP)-1, involved in the pathogenesis of atherosclerosis. Objective: Human AT cultures, isolated adipocytes, and stromal-vascular cells were used to investigate the relationship among AT-resident macrophages, MCP-1, and adiposity and the regulation of MCP-1. Results: mRNA levels of specific macrophage markers (CD68 and CD14) are correlated with adiposity in sc AT and visceral AT (P < 0.05). MCP-1 production is higher in stromal-vascular cells vs. adipocytes (P < 0.01) and correlates with macrophage markers in both AT compartments (P < 0.05). MCP-1 release is higher in obese subjects (P < 0.05) and in VAT (P < 0.01), but after adjusting for AT-resident macrophages, the differences disappear. MCP-1 is stimulated by IL-1β, TNF-α, IL-8, IL-4, and IL-6 + IL-6-soluble receptor and is decreased by dexamethasone, IL-10, metformin, and thiazolidinediones. Discussion: MCP-1 is correlated with specific macrophage markers, adiposity, and AT localization, but the relationship seems to be related to the number of AT-resident macrophages. Despite this, MCP-1 may be involved in obesity-related health complications, and the decrease of MCP-1 by metformin and thiazolidinediones suggests that these antidiabetic compounds have antiinflammatory properties improving the low-grade inflammatory state observed in obesity.

scholarly journals The Relationships of Plasma Adiponectin with a Favorable Lipid Profile, Decreased Inflammation, and Less Ectopic Fat Accumulation Depend on Adiposity

2006 ◽  
Vol 52 (10) ◽  
pp. 1934-1942 ◽  
Author(s):  
Konstantinos Kantartzis ◽  
Killian Rittig ◽  
Bernd Balletshofer ◽  
Jürgen Machann ◽  
Fritz Schick ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Endothelial Function ◽  
Visceral Adipose Tissue ◽  
Hdl Cholesterol ◽  
Ectopic Fat ◽  
Fat Accumulation ◽  
Plasma Adiponectin ◽  
Markers Of Inflammation ◽  
Visceral Adipose ◽  
The Relationship

Abstract Background: The metabolic effects of adiponectin, including insulin sensitivity, seem to become stronger with increasing adiposity. Adiposity may also affect the relationship of adiponectin concentrations with serum lipid profile; markers of inflammation, atherosclerosis, and endothelial function; and ectopic fat accumulation. Methods: We measured plasma adiponectin concentrations, serum lipids, and serum markers of inflammation, atherosclerosis, and endothelial function in 242 Caucasians without type 2 diabetes. We also measured visceral adipose tissue with magnetic resonance tomography and liver and intramyocellular fat with 1H magnetic resonance spectroscopy. Results: We divided the study participants into 2 groups: lean [mean (SE) total body fat, 26% (0.6%); n = 119] and obese [36% (0.6%); n = 123]. In the obese group, plasma adiponectin concentrations showed a strong positive association with concentrations of HDL cholesterol (P <0.0001) and negative associations with LDL cholesterol, triglycerides, high-sensitivity C-reactive protein, interleukin 6, apolipoprotein B100, soluble E-selectin, soluble vascular cellular adhesion molecule 1, plasminogen activator inhibitor 1, leukocyte count, and liver and intramyocellular fat (all P <0.03). In the lean group, adiponectin showed a less strong association with HDL cholesterol (P = 0.005) and liver fat (P = 0.03) and no significant associations with the other variables (all P >0.10). High visceral adipose tissue was a strong predictor of low adiponectin concentrations, particularly in the obese group, and attenuated many of the significant relationships. Conclusions: High adiponectin plasma concentrations are associated with favorable lipid profiles, decreased subclinical inflammation, decreased markers of atherosclerosis and endothelial function, and low ectopic fat accumulation, particularly in obese persons. Adiponectin may also have a concentration-related effect on the relationship between visceral adipose tissue and these metabolic characteristics, especially in obese persons.

scholarly journals Adipocyte Size Evaluation Based on Photoacoustic Spectral Analysis Combined with Deep Learning Method

2018 ◽  
Vol 8 (11) ◽  
pp. 2178 ◽  
Author(s):  
Xiang Ma ◽  
Meng Cao ◽  
Qinghong Shen ◽  
Jie Yuan ◽  
Ting Feng ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Deep Learning ◽  
Disease Risk ◽  
Spectral Band ◽  
Human Adipose Tissue ◽  
Learning Method ◽  
Adipocyte Size ◽  
Spectral Bands ◽  
Tissue Specimens ◽  
The Relationship

Adipocyte size, i.e., the cell area of adipose tissue, is correlated directly with metabolic disease risk in obese humans. This study proposes an approach of processing the photoacoustic (PA) signal power spectrum using a deep learning method to evaluate adipocyte size in human adipose tissue. This approach has the potential to provide noninvasive assessment of adipose tissue dysfunction, replacing traditional invasive methods of evaluating adipose tissue via biopsy and histopathology. A deep neural network with fully connected layers was used to fit the relationship between PA spectrum and average adipocyte size. Experiments on human adipose tissue specimens were performed, and the optimal parameters of the deep learning method were applied to establish the relationship between the PA spectrum and average adipocyte size. By studying different spectral bands in the entire spectral range using the deep network, a spectral band mostly sensitive to the adipocyte size was identified. A method of combining all frequency components of PA spectrum was tested to achieve a more accurate evaluation.

The Ras-homologous GTPase Arfrp1 is involved in the regulation of triglyceride storage in mouse and human adipose tissue

2009 ◽  
Vol 4 (S 01) ◽  
Author(s):  
A Hommel ◽  
D Hesse ◽  
M Blüher ◽  
T Engel ◽  
C Zahn ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue

Global methylation level in human adipose tissue is related to fat distribution

2013 ◽  
Vol 8 (S 01) ◽  
Author(s):  
M Keller ◽  
D Schleinitz ◽  
I Müller ◽  
M Stumvoll ◽  
P Kovacs ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Methylation Level ◽  
Human Adipose Tissue ◽  
Fat Distribution ◽  
Global Methylation ◽  
Global Methylation Level

IN VITRO STUDIES ON LIPOLYSIS IN HUMAN ADIPOSE TISSUE

1969 ◽  
Vol 61 (1_Suppl) ◽  
pp. S156
Author(s):  
Suad Efendić ◽  
Peter Amer ◽  
Jan Östman
Keyword(s):  
Adipose Tissue ◽  
Human Adipose Tissue ◽  
In Vitro Studies
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