scholarly journals LipiD-QuanT: a novel method to quantify lipid accumulation in live cells

2015 ◽  
Vol 56 (11) ◽  
pp. 2206-2216 ◽  
Author(s):  
Hilal Varinli ◽  
Megan J. Osmond-McLeod ◽  
Peter L. Molloy ◽  
Pascal Vallotton
Keyword(s):  
Lipid Accumulation ◽  
Live Cells ◽  
Novel Method

scholarly journals Poly(catecholamine) Coating and Biofunctionalization of CsPbBr3 Microcrystals

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

scholarly journals Poly(catecholamine) coated CsPbBr3 perovskite microlasers: lasing in water and biofunctionalization

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

Synchrotron-FTIR Microspectroscopy Enables the Distinction of Lipid Accumulation in Thraustochytrid Strains Through Analysis of Individual Live Cells

Protist ◽  
2015 ◽  
Vol 166 (1) ◽  
pp. 106-121 ◽  
Author(s):  
Jitraporn Vongsvivut ◽  
Philip Heraud ◽  
Adarsha Gupta ◽  
Tamilselvi Thyagarajan ◽  
Munish Puri ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Live Cells ◽  
Ftir Microspectroscopy

scholarly journals Poly(catecholamine) Coating and Biofunctionalization of CsPbBr3 Microcrystals

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

scholarly journals Poly(catecholamine) Coating and Biofunctionalization of CsPbBr3 Microcrystals

2021 ◽  
Author(s):  
Sangyeon Cho ◽  
Seok-Hyun Yun
Keyword(s):  
Lipid Bilayers ◽  
Environmental Stability ◽  
Live Cells ◽  
Functional Coatings ◽  
Common Strategy ◽  
Novel Method ◽  
Halide Perovskites ◽  
The Stability ◽  
New Applications

<p>Lead halide perovskites (LHP) microcrystals are promising materials for various optoelectronic applications. Surface coating on particles is a common strategy to improve their functionality and environmental stability, but LHP is not amenable to most coating chemistries because of its intrinsic weakness against polar solvents. Here, we describe a novel method of synthesizing LHP microcrystals in a super-saturated polar solvent using sonochemistry and applying various functional coatings on individual microcrystals <i>in situ</i>. We synthesize cesium lead bromine perovskite (CsPbBr<sub>3</sub>) microparticles capped with organic poly-norepinephrine (pNE) layers. The catechol group of pNE coordinates to bromine-deficient lead atoms, forming a defect-passivating and diffusion-blocking shell. The pNE layer enhances the stability of CsPbBr<sub>3</sub> in water by 2,000-folds, enabling bright luminescence and lasing from single microcrystals in water. Furthermore, the pNE shell permits biofunctionalization with proteins, small molecules, and lipid bilayers. Luminescence from CsPbBr<sub>3</sub> microcrystals is sustained in water over 1 hour and observed in live cells. The functionalization method may enable new applications of LHP particles in water-rich environments.<b></b></p>

Optical Nanosensing of Lipid Accumulation due to Enzyme Inhibition in Live Cells

ACS Nano ◽  
2019 ◽  
Vol 13 (8) ◽  
pp. 9363-9375 ◽  
Author(s):  
Vesna Živanović ◽  
Stephan Seifert ◽  
Daniela Drescher ◽  
Petra Schrade ◽  
Stephan Werner ◽  
...  
Keyword(s):  
Enzyme Inhibition ◽  
Lipid Accumulation ◽  
Live Cells

scholarly journals A novel method for monitoring functional lesion-specific recruitment of repair proteins in live cells

2015 ◽  
Vol 775 ◽  
pp. 48-58 ◽  
Author(s):  
Jordan Woodrick ◽  
Suhani Gupta ◽  
Pooja Khatkar ◽  
Kalpana Dave ◽  
Darya Levashova ◽  
...  
Keyword(s):  
Live Cells ◽  
Novel Method

scholarly journals Nessys: a novel method for accurate nuclear segmentation in 3D

2018 ◽  
Author(s):  
Guillaume Blin ◽  
Daina Sadurska ◽  
Rosa Portero Migueles ◽  
Naiming Chen ◽  
Julia Alice Watson ◽  
...  
Keyword(s):  
Three Dimensions ◽  
Live Cells ◽  
Computer Memory ◽  
Adult Tissue ◽  
Manual Curation ◽  
Trained Classifier ◽  
History Of ◽  
Novel Method ◽  
User Friendly ◽  
Nuclear Segmentation

Methods for measuring the properties of individual cells within their native 3D environment will enable a deeper understanding of embryonic development, tissue regeneration, and tumorigenesis. However current methods for segmenting nuclei in 3D tissues are not designed for situations where nuclei are densely packed, non-spherical, heterogeneous in shape, size, or texture, all of which are true of many embryonic and adult tissue types as well as in many cases for cells differentiating in culture. Here we overcome this bottleneck by devising a novel method based on labelling the nuclear envelope (NE) and automatically distinguishing individual nuclei using a tree structured ridge tracing method followed by shape ranking according to a trained classifier. The method is fast and makes it possible to process images that are larger than computer memory. We consistently obtain accurate segmentation rates of >90% even for challenging images such as mid-gestation embryos or 3D cultures. We provide a 3D editor and inspector for the manual curation of the segmentation results as well as a program to assess the accuracy of the segmentation. We have also generated a live reporter of the NE that can be used to track live cells in three dimensions over time. We use this to monitor the history of cell interactions and occurrences of neighbour exchange within cultures of pluripotent cells during differentiation. We provide these tools in an open-access user-friendly format.

MAPLE Direct Write (DW) patterning of Bio-active Ceramic Nanocomposites

2004 ◽  
Vol 845 ◽  
Author(s):  
A. Doraiswamy ◽  
T. M. Patz ◽  
R. J. Narayan ◽  
L. Harris ◽  
R. Auyeung ◽  
...  
Keyword(s):  
Ceramic Composite ◽  
Live Cells ◽  
Direct Write ◽  
Direct Writing ◽  
Cad Cam ◽  
A Cell ◽  
Novel Method ◽  
Heterogeneous Tissue ◽  
Laser Direct Write ◽  
Hydroxyl Apatite

ABSTRACTA laser-based approach, Matrix Assisted Pulsed Laser Direct Write (MAPLE) technique is used to demonstrate two-dimensional direct writing of ceramics such as hydroxyl-apatite and zirconia, for developing inorganic scaffolds. We also demonstrate the patterning of live MG63 osteoblast cells onto various substrates. Our results show successful direct writing of ceramics and live cells concurrently, with a growth profile similar to that of as-deposited cells. After several days of growth, a live/dead assay shows live cells suggesting the biocompatibility of the ceramic and the viability of the process. This investigation demonstrates a novel method of developing heterogeneous tissue scaffolds, such as a cell-ceramic composite by CAD/CAM patterns.

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