Expression of Gal4-dependent transgenes in cells of the mononuclear phagocyte system labeled with enhanced cyan fluorescent protein usingCsf1r-Gal4VP16/UAS-ECFP double-transgenic mice

2007 ◽  
Vol 83 (2) ◽  
pp. 430-433 ◽  
Author(s):  
Dmitry A. Ovchinnikov ◽  
Wendy J. M. van Zuylen ◽  
Claire E. E. DeBats ◽  
Kylie A. Alexander ◽  
Stuart Kellie ◽  
...  
Keyword(s):  
Transgenic Mice ◽  
Fluorescent Protein ◽  
Mononuclear Phagocyte ◽  
Mononuclear Phagocyte System ◽  
Cyan Fluorescent Protein ◽  
Double Transgenic Mice ◽  
Enhanced Cyan Fluorescent Protein ◽  
In Cells

scholarly journals Specific expression of an oxytocin-enhanced cyan fluorescent protein fusion transgene in the rat hypothalamus and posterior pituitary

2009 ◽  
Vol 204 (3) ◽  
pp. 275-285 ◽  
Author(s):  
Akiko Katoh ◽  
Hiroaki Fujihara ◽  
Toyoaki Ohbuchi ◽  
Tatsushi Onaka ◽  
W Scott Young ◽  
...  
Keyword(s):  
Fluorescent Protein ◽  
Fusion Gene ◽  
Plasma Osmolality ◽  
Cyan Fluorescent Protein ◽  
Posterior Pituitary ◽  
Protein Fusion ◽  
Transgenic Rats ◽  
Salt Loading ◽  
Wide Range ◽  
Enhanced Cyan Fluorescent Protein

We have generated rats bearing an oxytocin (OXT)-enhanced cyan fluorescent protein (eCFP) fusion transgene designed from a murine construct previously shown to be faithfully expressed in transgenic mice. In situ hybridisation histochemistry revealed that the Oxt–eCfp fusion gene was expressed in the supraoptic nucleus (SON) and the paraventricular nucleus (PVN) in these rats. The fluorescence emanating from eCFP was observed only in the SON, the PVN, the internal layer of the median eminence and the posterior pituitary (PP). In in vitro preparations, freshly dissociated cells from the SON and axon terminals showed clear eCFP fluorescence. Immunohistochemistry for OXT and arginine vasopressin (AVP) revealed that the eCFP fluorescence co-localises with OXT immunofluorescence, but not with AVP immunofluorescence in the SON and the PVN. Although the expression levels of the Oxt–eCfp fusion gene in the SON and the PVN showed a wide range of variations in transgenic rats, eCFP fluorescence was markedly increased in the SON and the PVN, but decreased in the PP after chronic salt loading. The expression of the Oxt gene was significantly increased in the SON and the PVN after chronic salt loading in both non-transgenic and transgenic rats. Compared with wild-type animals, euhydrated and salt-loaded male and female transgenic rats showed no significant differences in plasma osmolality, sodium concentration and OXT and AVP levels, suggesting that the fusion gene expression did not disturb any physiological processes. These results suggest that our new transgenic rats are a valuable new tool to identify OXT-producing neurones and their terminals.

scholarly journals Crystal structure of the cyan fluorescent protein Cerulean-S175G

2016 ◽  
Vol 72 (7) ◽  
pp. 516-522 ◽  
Author(s):  
Sang-wook Park ◽  
Sunghyun Kang ◽  
Tae-Sung Yoon
Keyword(s):  
Crystal Structure ◽  
Crystal Structures ◽  
Conformational Changes ◽  
Fluorescent Protein ◽  
Detailed Comparison ◽  
Cyan Fluorescent Protein ◽  
Double Mutation ◽  
Aequorea Victoria ◽  
Green Fluorescent ◽  
Enhanced Cyan Fluorescent Protein

Enhanced cyan fluorescent protein (ECFP) was derived fromAequorea victoriagreen fluorescent protein (avGFP), notably with S65T/Y66W mutations. Its chromophore consists of a tripeptide comprised of Thr65, Trp66 and Gly67 (TWG) residues, while that ofavGFP consists of a Ser65, Tyr66 and Gly67 (SYG) tripeptide. Cerulean and SCFP3A were derived from ECFP-S72A/H148D (a double mutation) with additional Y145A and S175G mutations, respectively, while Cerulean-S175G has both mutations (Y145A and S175G). The crystal structures of these ECFP variants at neutral pH were reported to adopt two distinct major conformations calledECFPandCerulean. In this study, Cerulean-S175G was revealed to adopt only theCeruleanconformation, while Cerulean has been reported to adopt both theECFPand theCeruleanconformations in its crystal structures. Sharing the same S175G mutation with SCFP3A, Cerulean-S175G showed a slightly increased quantum yield, like SCFP3A, but did not adopt theECFPconformation adopted by SCFP3A. Detailed comparison of Cerulean-S175G and other ECFP variants revealed that the notable conformational changes in ECFP variants can be understood mainly in terms of the interaction between the Trp66 residue of the chromophore and residues 145–148 of β-strand 7.

The enhanced cyan fluorescent protein: a sensitive pH sensor for fluorescence lifetime imaging

2013 ◽  
Vol 405 (12) ◽  
pp. 3983-3987 ◽  
Author(s):  
Sandrine Poëa-Guyon ◽  
Hélène Pasquier ◽  
Fabienne Mérola ◽  
Nicolas Morel ◽  
Marie Erard
Keyword(s):  
Fluorescence Lifetime ◽  
Fluorescent Protein ◽  
Protein A ◽  
Ph Sensor ◽  
Cyan Fluorescent Protein ◽  
Fluorescence Lifetime Imaging ◽  
Lifetime Imaging ◽  
Enhanced Cyan Fluorescent Protein

scholarly journals INFLUENCE OF MAGNETOMICELLS BASED ON IRON NANOPARTICLES COATED BY CARBON ON STRUCTURE OF RAT LIVER

2015 ◽  
Vol 14 (2) ◽  
pp. 5-11
Author(s):  
G. Yu. Vasyukov ◽  
I. V. Milto ◽  
I. V. Sukhodolo ◽  
I. V. Mitrofanova
Keyword(s):  
Histological Examination ◽  
Intravenous Injection ◽  
Rat Liver ◽  
Iron Nanoparticles ◽  
Mononuclear Phagocyte ◽  
Mononuclear Phagocyte System ◽  
Degenerative Changes ◽  
Histochemical Reaction ◽  
Single Intravenous Injection ◽  
In Cells

In this paper we study the effect of a single intravenous injection of suspension of magnitomitsell based on iron nanoparticles modified with carbon on the structure of the rat liver. Histological examination revealed the hemodynamic disturbances in the stroma of the organ and degenerative changes of hepatocytes from 1 to 40 days of the experiment. Perls histochemical reaction demonstrated the accumulation of modified iron nanoparticles in cells of the mononuclear phagocyte system of the liver. Number of Perlspositive macrophages decreased during the experiment from 1 to 40 per day.

Cyan fluorescent protein (CFP) expressing cells in the retina of Thy1-CFP transgenic mice before and after optic nerve injury

2010 ◽  
Vol 468 (2) ◽  
pp. 110-114 ◽  
Author(s):  
Xu Wang ◽  
Michele L. Archibald ◽  
Kelly Stevens ◽  
William H. Baldridge ◽  
Balwantray C. Chauhan
Keyword(s):  
Optic Nerve ◽  
Transgenic Mice ◽  
Nerve Injury ◽  
Fluorescent Protein ◽  
Cyan Fluorescent Protein ◽  
Optic Nerve Injury ◽  
Before And After
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