scholarly journals Gene expression changes with differentiation of cord blood stem cells to respiratory epithelial cells: a preliminary observation

2011 ◽  
Vol 2 (2) ◽  
pp. 19 ◽  
Author(s):  
Michael J Berger ◽  
Sharon R Minnerath ◽  
Sheryl D Adams ◽  
Barbara M Tigges ◽  
Stacey L Sprague ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Epithelial Cells ◽  
Cord Blood ◽  
Preliminary Observation ◽  
Respiratory Epithelial Cells ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells ◽  
Respiratory Epithelial

scholarly journals Comparison of Signaling Pathways Gene Expression in CD34−Umbilical Cord Blood and Bone Marrow Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-13 ◽  
Author(s):  
Rafał Stojko ◽  
Monika Bojdys-Szyndlar ◽  
Agnieszka Drosdzol-Cop ◽  
Andrzej Madej ◽  
Krzysztof Wilk
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Cord Blood ◽  
Signaling Pathways ◽  
Signaling Pathway ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Biological Processes ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

The aim of the study was to compare the biological activity of the total pool of genes in CD34−umbilical cord blood and bone marrow stem cells and to search for the differences in signaling pathway gene expression responsible for the biological processes. The introductory analysis revealed a big similarity of gene expression among stem cells. When analyzing GO terms for biological processes, we observed an increased activity of JAK-STAT signaling pathway, calcium-mediated, cytokine-mediated, integrin-mediated signaling pathway, and MAPK in a cluster of upregulating genes in CD34−umbilical cord blood stem cells. At the same time, we observed a decreased activity of BMP signaling pathways, TGF-beta pathway, and VEGF receptor signaling pathway in a cluster of downregulating genes in CD34−umbilical cord blood stem cells. In accordance with KEGG classification, the cytokine-cytokine receptor interaction, toll-like receptor signaling pathway, and JAK-STAT signaling pathway are overrepresented in CD34−umbilical cord blood stem cells. A similar gene expression in both CD34−UCB and BM stem cells was characteristic for such biological processes as cell division, cell cycle gene expression, mitosis, telomere maintenance with telomerase, RNA and DNA treatment processes during cell division, and similar genes activity of Notch and Wnt signaling pathways.

Effect of Human Umblical Cord Blood Stem Cells on a Rodent Model of Parkinson's Disease

2018 ◽  
Vol 46 (2) ◽  
pp. 117-127
Author(s):  
Somia Abd-Allah ◽  
El-Sayed Abdel-Aziz ◽  
Sabry Ali ◽  
Gamal El-Din Shams ◽  
Hesham Mohammed ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Stem Cells ◽  
Parkinson's Disease ◽  
Cord Blood ◽  
Rodent Model ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Neuronal Apoptosis Is Inhibited by Cord Blood Stem Cells after Spinal Cord Injury

2009 ◽  
Vol 26 (11) ◽  
pp. 2057-2069 ◽  
Author(s):  
Venkata Ramesh Dasari ◽  
Krishna Kumar Veeravalli ◽  
Andrew J. Tsung ◽  
Christopher S. Gondi ◽  
Meena Gujrati ◽  
...  
Keyword(s):  
Spinal Cord ◽  
Stem Cells ◽  
Spinal Cord Injury ◽  
Cord Blood ◽  
Neuronal Apoptosis ◽  
Cord Injury ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

scholarly journals From cord to caudate: characterizing umbilical cord blood stem cells and their paracrine interactions with the injured brain

2017 ◽  
Vol 83 (1-2) ◽  
pp. 205-213 ◽  
Author(s):  
Priya F Maillacheruvu ◽  
Lauren M Engel ◽  
Isaiah T Crum ◽  
Devendra K Agrawal ◽  
Eric S Peeples
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Injured Brain ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

scholarly journals IN UTERO TRANSPLANTATION OF HUMAN STEM CELLS USING AN ANIMAL MODEL: A COMPARISON BETWEEN TWO TECHNIQUES

2009 ◽  
Vol 15 (1) ◽  
pp. 5-10
Author(s):  
Daria GROZA ◽  
Emoke PALL ◽  
Mihai CENARIU ◽  
Raul POP ◽  
Nicolae Nicolae ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cord Blood ◽  
Loss Rate ◽  
Fetal Loss ◽  
In Utero ◽  
Needle Puncture ◽  
Ultrasound Guided ◽  
In Utero Transplantation ◽  
Blood Stem Cells ◽  
Cord Blood Stem Cells

Objective: The purpose of this study was to assess the feasibility of in utero stem cell transplantation of human umbilical cord blood stem cells in fetal sheep and to compare two different techniques of in utero transplantation, namely ultrasound-guided in utero transplantation and in utero transplantation after midline celiotomy. Study design: Umbilical cord blood units were collected from term deliveries, after obtaining written informed consent. Human cord blood–derived, CD34+ stem cells were injected into the peritoneal cavity of 60- to 65-day-old ovine fetuses by using 2 different techniques: ultrasound-guided transabdominal percutaneous needle puncture and midline celiotomy with the exposure of the pregnant uterus. Engraftment was determined after birth by flow cytometry with use of human-specific anti-CD 34/45 antibodies. Results: We obtained a total of 3 chimeric lambs. Using the midline celiotomy technique the fetal loss rate was 75% and only 33,3% when using ultrasound-guided transabdominal percutaneous needle puncture technique. Engraftment of donor cells was found in all fetuses, with a mean level of 1.4% in fetal peripheral blood and 3.3% in fetal bone marrow. Conclusion: This preliminary study indicates that in utero stem cell transplantation of human hematopoietic cord blood stem cells in fetal lambs is feasible and effective in terms of hematopoietic engraftment. We also concluded that the ultrasound-guided transabdominal percutaneous needle puncture technique is more effective than performing a midline celiotomy in terms of fetal loss rate.

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