scholarly journals Validation of polymerase chain reaction in experimental sepsis diagnosis beyond blood culture

Critical Care ◽  
2008 ◽  
Vol 12 (Suppl 5) ◽  
pp. P47
Author(s):  
Marcello Ruiz-Silva ◽  
Derci Sa-Filho ◽  
Marcos Caseiro ◽  
Ivan Koh
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Experimental Sepsis ◽  
Chain Reaction ◽  
Sepsis Diagnosis ◽  
Polymerase Chain

scholarly journals Polymerase Chain Reaction and blood culture for diagnosis of canine sepsis

2018 ◽  
Vol 48 (6) ◽  
Author(s):  
Marcelo Marques da Silveira ◽  
Stéfhano Luis Cândido ◽  
Karin Rinaldi dos Santos ◽  
Maerle Oliveira Maia ◽  
Roberto Lopes de Souza ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Diagnostic Value ◽  
Turnaround Time ◽  
Diagnostic Techniques ◽  
Pcr Analysis ◽  
Chain Reaction ◽  
Suspected Sepsis ◽  
Fungal Dna ◽  
Polymerase Chain

ABSTRACT: Sepsis is characterized by the presence of organ dysfunction secondary to the dysregulated systemic inflammatory response associated with an infection, and has high mortality rates. Traditional diagnostic techniques based on non-microbiological isolation are time-consuming and may delay treatment. Thus, this study aimed to compare bacterial and fungal broad-range polymerase chain reaction (PCR) and blood culture for diagnosis of sepsis in dogs. Blood samples from 88 dogs with suspected sepsis were analyzed by blood culture, and PCR to detect bacterial and fungal DNA. On blood culture, 20 (22.7%) samples tested positive for bacterial isolates; however, none tested positive for fungi. Through PCR analysis, bacterial DNA was detected in 46 (52.3%) animals, whereas fungal DNA was present in one (1.1%) sample. Our results showed that PCR-based testing has important diagnostic value for canine blood infections because it has a shorter turnaround time and higher sensitivity than traditional blood culture.

scholarly journals Comparison of results obtained by widal agglutination test & polymerase chain reaction among clinically suspected typhoid fever cases

2015 ◽  
Vol 30 (2) ◽  
pp. 46-50
Author(s):  
Shafinaz Khan ◽  
Md Ruhul Amin Miah ◽  
Shammin Haque ◽  
Chowdhury Rafia Naheen
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Typhoid Fever ◽  
False Negative ◽  
Salmonella Typhi ◽  
Widal Test ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Positive Results ◽  
Prior Antibiotic Treatment

The diagnosis of typhoid fever currently depends on isolation of Salmonella Typhi from blood. The sensitivity of blood culture is very low due to prior antibiotic treatment which is a common practice in Bangladesh. The sensitivity of blood culture also decreases at later stage of the disease. Widal test is the most utilized test in Bangladesh next to blood culture because it is inexpensive, less invasive. But the result of the test is controversial due to false negative & false positive results in some cases.  In this study, a recently introduced polymerase chain reaction-based technique (which has 100% specificity for S. Typhi) was compared with widal test among 80 clinically suspected typhoid fever cases.  Among 80 cases, the respective figures of positivity for PCR & widal test were 70% & 43.75% respectively.  It can be concluded that PCR based technique is more sensitive & much superior to widal for diagnosis of typhoid fever. DOI: http://dx.doi.org/10.3329/bjpp.v30i2.22683 Bangladesh J Physiol Pharmacol 2014; 30(2): 46-50

Diagnosis of neonatal sepsis using 16S rRNA polymerase chain reaction

2017 ◽  
Vol 47 (4) ◽  
pp. 336-339 ◽  
Author(s):  
Harish Punia ◽  
Geeta Gathwala ◽  
Dhara B Dhaulakhandi ◽  
Mohammed Aamir
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
16S Rrna ◽  
Neonatal Sepsis ◽  
Predictive Value ◽  
Tertiary Care ◽  
College Teaching ◽  
Turnaround Time ◽  
Chain Reaction ◽  
Polymerase Chain

The gold standard for detecting bacterial sepsis is blood culture. However, the sensitivity of blood culture is low and the results take 48–72 h. Molecular assays for the detection of bacterial DNA permit early detection of a bacterial cause as the turnaround time is 6–8 h. We undertook an evaluation of the performance of universal bacterial primer (16S rRNA) polymerase chain reaction (PCR) in the diagnosis of neonatal sepsis at a tertiary care medical college teaching hospital. 16S rRNA PCR was positive in all cases of blood culture proven sepsis. PCR revealed 95.6% sensitivity, 100% specificity, 100% positive predictive value and 91.2% negative predictive value and so appears to be a useful tool for the early diagnosis of bacterial neonatal sepsis.

scholarly journals Randomized Trial of Rapid Multiplex Polymerase Chain Reaction–Based Blood Culture Identification and Susceptibility Testing

2015 ◽  
Vol 61 (7) ◽  
pp. 1071-1080 ◽  
Author(s):  
Ritu Banerjee ◽  
Christine B. Teng ◽  
Scott A. Cunningham ◽  
Sherry M. Ihde ◽  
James M. Steckelberg ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Randomized Trial ◽  
Multiplex Polymerase Chain Reaction ◽  
Susceptibility Testing ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Culture Identification

scholarly journals Blood Culture–Negative Cardiovascular Infection in a Patient With Multiple Sclerosis

2019 ◽  
Vol 6 (10) ◽  
Author(s):  
Cléa Melenotte ◽  
Ahmed Loukil ◽  
Audrey Rico ◽  
Hubert Lepidi ◽  
Didier Raoult
Keyword(s):  
Multiple Sclerosis ◽  
Polymerase Chain Reaction ◽  
Blood Culture ◽  
Chain Reaction ◽  
Serological Tests ◽  
Specific Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Testing ◽  
Polymerase Chain ◽  
Culture Negative

Abstract A patient with multiple sclerosis presented with seronegative C. burnetii endocarditis diagnosed using C. burnetii–specific polymerase chain reaction and fluorescence in situ hybridization on cardiovascular biopsy. This case supports the necessity of a systematic polymerase chain reaction testing of removed cardiac valves because blood culture–negative endocarditis can be pauci-symptomatic, and serological tests can be negative in cases of immunosuppression.

Sign in / Sign up

Export Citation Format

Share Document